EBNA-1、LMP-1基因分型与维吾尔族霍奇金淋巴瘤相关性研究

发布时间：2018-03-09 06:29

本文选题：维吾尔族　切入点：霍奇金淋巴瘤　出处：《新疆医科大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:探索EB病毒核心抗原-1(EBV nuclear antigen 1,EBNA-1)、潜伏膜蛋白-1(Latent membrane protein-1,LMP-1)基因分型与维吾尔族霍奇金淋巴瘤(Hodgkin's lymphoma,HL)发病的相关性。方法:采用原位杂交技术检测新疆肿瘤医院50例维吾尔族霍奇金淋巴瘤(HL)及60例淋巴结反应性增生(LRH)活检组织中EBV编码的小RNA 1(EBV-encoded small RNA 1,EBER1)以筛选EBV阳性淋巴瘤标本,采用PCR结合DNA测序技术对EBV阳性淋巴瘤标本进行EBV 1/2、C/D、F/f分型,并检测EBV阳性淋巴瘤标本中EBNA-1、LMP-1编码基因分型。结果:(1)50例HL病理标本中共有27例检测到EBER1阳性信号,阳性率为54.0%。60例LRH病理标本中共有8例检测到EBER1阳性信号,阳性率为13.3%。(2)EBV 1/2、C/D、F/f分型在HL与LRH组中分布无统计学差异(P0.05)。(3)两组共检测到3种亚型,包括V-val型,P-thr型以及P-ala型,3种EBNA-1亚型在HL与LRH组中分布无统计学差异(P=1.00);3种EBNA-1亚型在HL亚型分布无统计学差异(P=0.731)。(4)LMP-1包括4种亚型(China 1、China2、Med-、Med+)及重组病毒株型的分布在HL与LRH分布无显著差异(P=0.256);LMP-1亚型在各亚型HL分布差异无统计学意义(P=0.854)。结论:(1)EB病毒阳性维吾尔HL患者中,EB病毒感染以1型、F型感染为主。(2)EBV 1/2、C/D、F/f分型及EBNA-1、LMP-1亚型在病例组及对照组中分布均无显著性差异,表明EBV 1/2、C/D、F/f亚型及EBNA-1、LMP-1亚型可能不具有肿瘤特异性。(3)V-val是维吾尔族HL患者EBNA-1中最为常见亚型,也是维吾尔族LRH中最常见的类型。(4)China-1是维吾尔族HL患者LMP-1中最为常见亚型。
[Abstract]:Objective: to investigate the relationship between EBV core antigen -1EBV nuclear antigen 1EBNA-1, latent membrane protein-1LMP-1) and the pathogenesis of Hodgkin's lymphoma in Uygur nationality. Methods: in situ hybridization technique was used to detect the incidence of Hodgkinia lymphoma in 50 cases of tumor hospital in Xinjiang. Methods: to investigate the relationship between the gene typing of EBV core antigen -1EBV nuclear antigen 1, latent membrane protein-1 (LMP-1) and the pathogenesis of Hodgkinine lymphoma in Uygur nationality. EBV encoding small RNA 1 EBV-encoded small RNA 1 EBER1) was used to screen EBV positive lymphoma specimens from our own Hodgkin's lymphoma and 60 cases of lymphaden-reactive hyperplasia (LRH) biopsy tissues. PCR and DNA sequencing techniques were used to detect the EBV 1 / 2 C / DU F / f typing of EBV positive lymphoma samples, and EBNA-1 LMP-1 coding genotyping was detected in EBV positive lymphoma samples. Results the EBER1 positive signals were detected in 27 out of 50 HL specimens from 50 cases of HL. The positive rate was 54.0.The positive rate of EBER1 was detected in 8 out of 60 pathological specimens of LRH, and the positive rate was 13.32.The positive rate was 13.32.The positive rate was 13.32.There was no statistical difference between HL and LRH in the distribution of 1 / 2C / D / F / F / f typing and there was no significant difference between the two groups in the distribution of EBER1 subtypes. There was no significant difference in the distribution of three EBNA-1 subtypes between HL and LRH. There was no significant difference in the distribution of three EBNA-1 subtypes between HL and LRH. There was no significant difference in the distribution of three EBNA-1 subtypes in HL subtype P0. 731, P 0. 731. The LMP-1 including 4 subtypes, China 1, China 2 Med-Med) and the distribution of recombinant virus strains in HL and LRH. There was no significant difference in the distribution of LMP-1 subtypes in all subtypes of HL. Conclusion the infection of Epstein-Barr virus in Uygur HL patients with 1: 1 EB virus positive is mainly caused by type 1 serotype F infection and EBV 1 / 2% C / D / D / f typing and EBNA-1 LMP-1 subtype in the case group and the control group. There was no significant difference in middle distribution, The results showed that the subtypes of EBV 1 / 2 C / D / F / f and EBNA-1G LMP-1 may not be tumor-specific. The most common subtypes of EBNA-1 in Uygur patients with HL are the most common subtypes of EBNA-1, and the most common subtypes of LMP-1 in Uygur patients with HL.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R733.1

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