RNAi沉默Notch1基因对多发性骨髓瘤细胞致瘤性的影响

发布时间：2018-03-18 17:21

  本文选题：多发性骨髓瘤　切入点：Notch基因　出处：《中国实验血液学杂志》2017年06期 　论文类型：期刊论文

【摘要】：目的:探讨RNAi沉默Notch1基因对多发性骨髓瘤细胞在NOD/SCID小鼠体内致瘤性的影响。方法:使用shRNA方法靶向沉默骨髓瘤RPMI8226细胞Notch1基因,建立NOD/SCID小鼠多发性骨髓瘤模型,观察Notch1基因沉默后骨髓瘤的成瘤速度、大小的变化;用ELISA法检测荷瘤小鼠血清中IL-6和VEGF水平变化。结果:Notch1-shRNA沉默Notch1基因后肿瘤细胞成瘤速度明显减慢,肿瘤体积缩小,与对照组比较差异显著。实验组小鼠血清中IL-6和VEGF水平与对照组比较明显降低(P0.05)。结论:靶向沉默Notch1基因可显著抑制骨髓瘤细胞的致瘤能力,其机制可能与Notch1基因沉默后瘤细胞分泌IL-6和VEGF的能力降低有关,Notch1基因沉默有可能成为治疗多发性骨髓瘤的新策略。
[Abstract]:Objective: to investigate the effect of RNAi silencing Notch1 gene on the tumorigenicity of multiple myeloma cells in NOD/SCID mice. Methods: the NOD/SCID mouse model of multiple myeloma was established by targeting the Notch1 gene of RPMI8226 cells with shRNA method. The tumorigenesis speed and size of myeloma were observed after Notch1 gene silencing, and the changes of IL-6 and VEGF levels in serum of mice bearing tumor were detected by ELISA method. Results the tumorigenesis rate and tumor volume of tumor cells decreased after Notch1 gene silencing with Notch1 gene silencing. Compared with the control group, the serum levels of IL-6 and VEGF in the experimental group were significantly lower than those in the control group. Conclusion: the target silencing Notch1 gene can significantly inhibit the tumorigenicity of myeloma cells. The mechanism may be related to the decrease in the ability of tumor cells to secrete IL-6 and VEGF after Notch1 gene silencing, which may be a new strategy for the treatment of multiple myeloma.
【作者单位】： 厦门大学附属中山医院血液科;贵州医科大学第三附属医院血液科;
【基金】：国家自然科学基金(81172246) 贵州医科大学-省科技合作项目(黔科合LH[2016]7391)
【分类号】：R733.3

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