IL-10与IL-17水平在乙型肝炎病毒相关肝癌中的表达及临床价值研究

发布时间：2018-04-01 10:17

本文选题：肝癌　切入点：乙型肝炎病毒　出处：《中华医院感染学杂志》2017年16期

【摘要】：目的探讨白细胞介素-10(IL-10)、白细胞介素-17(IL-17)水平在乙型肝炎病毒相关性肝癌中的表达及其临床价值。方法选取2013年8月-2016年9月医院乙型肝炎病毒相关性肝癌患者104例作为研究组及同期健康体检者104名为对照组,比较乙型肝炎病毒相关性肝癌患者肝癌组织和癌旁组织及健康人群正常肝组织IL-10、IL-17表达及甲胎蛋白含量等。结果 IL-10在肝癌组织、癌旁组织中mRNA水平表达分别为(0.472±0.050)Gapdh、(0.022±0.004)Gapdh,高于健康人群的(0.010±0.002)Gapdh,IL-17在肝癌组织、癌旁组织中mRNA水平表达分别为(0.620±.0.087)Gapdh、(0.024±0.005)Gapdh,高于健康人群的(0.011±0.004)Gapdh,肝癌组织高于癌旁组织,差异均有统计学意义(P0.05),肝癌组织、癌旁组织中蛋白水平表达高于健康人群,肝癌组织高于癌旁组织,差异均有统计学意义(P0.05);在肝癌细胞株BEL-7402、HepG2中的水平高于正常肝细胞HL-7702,BEL-7402高于HepG2,差异均有统计学意义(P0.05);IL-10、IL-17在mRNA和蛋白水平表达与甲胎蛋白含量呈正相关性(P0.05)。结论 IL-10、IL-17高表达与乙型肝炎病毒相关性肝癌发生发展具有密切相关性,加强二者检测与干预,有助于乙型肝炎病毒相关性肝癌的诊断与疾病治疗,改善预后。
[Abstract]:Objective to investigate the expression and clinical value of interleukin-10 (IL-10) and interleukin-17 (IL-17) in patients with hepatitis B virus associated liver cancer. Methods 104 patients with hospital hepatitis B virus associated liver cancer from August 2013 to September 2016 were selected. As the study group and the same period of health examination 104 as the control group, To compare the expression of IL-10 IL-17 and alpha-fetoprotein in liver tissues of patients with Hepatitis B virus associated hepatocellular carcinoma (HCC), adjacent tissues and healthy people. Results the expression of IL-10 IL-17 and the content of alpha-fetoprotein were detected in HCC tissues. The expression of mRNA in paracancerous tissues was 0.472 卤0.050 and 0.022 卤0.004 respectively, which was higher than that in healthy controls (0.010 卤0.002). The expression of mRNA in adjacent tissues was 0.620 卤.0.087 Gapdh0.024 卤0.005, higher than that in healthy controls (0.011 卤0.004), and the difference was statistically significant (P 0.05). The expression of protein in paracancerous tissues was higher than that in healthy people, and that in HCC tissues was higher than that in paracancerous tissues. The level of IL-10 IL-17 in the hepatoma cell line BEL-7402 was higher than that in the normal hepatocyte HL-7702BEL-7402, and there was a positive correlation between the expression of IL-10 IL-17 in mRNA and protein levels and the content of alpha-fetoprotein (P0.055.Conclusion IL-10IL-17 is higher than that of normal hepatocyte HL-7702BEL-7402). It is closely related to the occurrence and development of Hepatitis B virus related liver cancer. Strengthening the detection and intervention is helpful for the diagnosis and treatment of hepatitis B virus associated liver cancer and the improvement of prognosis.
【作者单位】：平顶山市第二人民医院感染性疾病科;平顶山市第二人民医院呼吸科;许昌市襄城县人民医院手术室;
【分类号】：R512.62;R735.7

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