miR-551b在人胃癌组织中的表达及对胃癌细胞凋亡的影响

发布时间：2018-04-01 10:18

  本文选题：微小RNA　切入点：胃癌　出处：《华中科技大学学报(医学版)》2017年04期

【摘要】：目的探讨miR-551b在人胃癌组织中的表达及对胃癌细胞凋亡的影响。方法采用Real-time PCR检测胃癌组织、正常胃组织miR-551b表达水平,分别将miRNA无义序列、miR-551bmimics、miR-551binhibitors转染至人胃癌细胞株SGC-7901,Real-time PCR检测各组细胞miR-551b表达,MTT法检测各组肿瘤细胞增殖活性,Transwell法检测各组细胞侵袭能力,流式细胞术检测各组细胞凋亡情况,Hoechst33342荧光染色观察各组细胞自噬、凋亡的发生,Western blot检测各组细胞NF-κB、LC3Ⅱ、Beclin 1蛋白表达。结果 miR-551b在胃癌组织表达明显下调,胃癌组织miR-551b相对表达量(1.75±0.13)显著低于正常胃组织(2.47±0.38)(P0.05)。与NC组和miR-551binhibitors组比较,miR-551bmimics组miR-551b表达水平显著上升,细胞增殖率和侵袭率显著降低,细胞凋亡率明显上升,各指标比较差异均具有统计学意义(均P0.05)。Hoechst33342荧光染色显示miR-551bmimics组出现大量自噬泡,NC组可见部分自噬泡,而miR-551binhibitors组仅有少量自噬泡。Western blot检测结果显示,与NC组和miR-551binhibitors组比较,miR-551bmimics组NF-κB、LC3Ⅱ、Beclin 1蛋白表达水平显著升高(均P0.05)。结论 miR-551b能够抑制胃癌细胞的增殖和侵袭等细胞生物功能,其作用机制可能是诱导了胃癌细胞发生自噬性凋亡。
[Abstract]:Objective to investigate the expression of miR-551b in human gastric carcinoma and its effect on the apoptosis of gastric cancer cells. Methods Real-time PCR was used to detect the expression of miR-551b in gastric cancer and normal gastric tissues. MiRNA nonsense sequence was transfected into human gastric cancer cell line SGC-7901Real-time PCR. MiR-551b expression in each group was detected. The proliferation activity of tumor cells in each group was detected by miRNA method and the invasiveness of tumor cells in each group was detected by Transwell method. Apoptosis was detected by flow cytometry and autophagy was observed by Hoechst33342 fluorescence staining. Apoptosis was detected by Western blot. The expression of NF- 魏 BnLC3 鈪，

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