IGF-1R和VEGFR2蛋白在结直肠癌组织中的表达及其临床意义

发布时间：2018-04-01 22:15

本文选题：结直肠癌　切入点：胰岛素样生长因子受体　出处：《中国药房》2017年35期

【摘要】：目的:观察胰岛素样生长因子1受体(IGF-1R)和血管内皮细胞生长因子受体2(VEGFR2)蛋白在结直肠癌组织中的表达,并探讨其临床意义。方法:选择2011年1月-2012年12月在我院行手术治疗的结直肠癌患者98例,收集其肿瘤组织和癌旁组织,采用免疫组化链霉菌抗生物素蛋白-过氧化物酶连接法检测其IGF-1R和VEGFR2蛋白的表达水平,采用逆转录聚合酶链反应法检测两者m RNA的表达水平;考察IGF-1R和VEGFR2蛋白表达与结直肠癌患者临床病理特征的相关性,以及其对术后辅助化疗患者4年无疾病生存率的影响。结果:结直肠癌患者肿瘤组织中IGF-1R和VEGFR2蛋白的阳性表达率分别为66.3%和60.2%,而癌旁组织中均未见其表达,组间比较差异均有统计学意义(P0.05)。肿瘤组织中IGF-1R m RNA和VEGFR2 m RNA的相对表达量均显著高于癌旁组织,组间比较差异均有统计学意义(P0.05),且两者m RNA在肿瘤组织中的表达水平呈正相关(r=0.729,P0.05)。IGR-1R蛋白的表达与患者的性别、年龄、肿瘤部位、有无脉管癌栓形成无关(P0.05),而与肿瘤组织学分级、浸润深度和有无淋巴结转移有关(P0.05);VEGFR2蛋白的表达与患者的性别、年龄无关(P0.05),而与肿瘤部位、组织学分级、浸润深度、有无淋巴结转移和脉管癌栓形成有关(P0.05)。98例结直肠癌患者中,14例患者术后未行化疗;其余84例患者4年无疾病生存率为76.2%(64/84),其中IGF-1R蛋白阴性表达、VEGFR2蛋白阴性表达患者的4年无疾病生存率(82.8%、78.8%)均显著高于其阳性表达者(72.7%、74.5%),差异均有统计学意义(P0.05)。采用多因素Logistic回归分析对性别、年龄、组织学分级、肿瘤部位、浸润深度、有无淋巴结转移和脉管癌栓形成等因素进行校正后,IGF-1R和VEGFR2蛋白的表达仍与患者4年无疾病生存率相关[校正比值比(OR)分别为2.31、2.15,95%置信区间分别为(0.57,4.15)、(0.45,4.03),P0.05]。结论:结直肠癌患者肿瘤组织中IGF-1R和VEGFR2蛋白及m RNA的表达明显增强,且蛋白的表达与肿瘤组织学分级、浸润深度、有无淋巴结转移等因素以及术后辅助化疗患者的预后有关。两者的联合检测可为结直肠癌患者的靶向治疗提供参考。
[Abstract]:Objective: to investigate the expression of insulin-like growth factor-1 receptor (IGF-1R) and vascular endothelial growth factor receptor (VEGFR2) protein in colorectal carcinoma. Methods: 98 patients with colorectal cancer who underwent surgical treatment in our hospital from January 2011 to December 2012 were selected and their tumor tissues and adjacent tissues were collected. The expression levels of IGF-1R and VEGFR2 proteins were detected by immunohistochemical streptomyces biotin-peroxidase linkage method and m RNA expression levels were detected by reverse transcriptase polymerase chain reaction (RT-PCR). To investigate the correlation between the expression of IGF-1R and VEGFR2 protein and the clinicopathological features of colorectal cancer patients. Results: the positive expression rates of IGF-1R and VEGFR2 protein were 66.3% and 60.2%, respectively, in the tumor tissues of patients with colorectal cancer, but they were not found in the adjacent tissues. The relative expressions of IGF-1R m RNA and VEGFR2 m RNA in tumor tissues were significantly higher than those in paracancerous tissues. The expression level of m RNA in tumor tissues was positively correlated with sex, age, tumor site, tumor site and tumor grade, but was not correlated with tumor histology grade, the expression level of m RNA in tumor tissues was positively correlated with the expression of IGR-1R protein, which was not related to the patient's sex, age, tumor site, tumor site, or not, but not related to the histological grade of the tumor, and there was no correlation between the expression of m RNA and the tumor tissue grade, and there was no significant difference between the two groups. The expression of VEGFR2 protein related to the depth of invasion and lymph node metastasis was not related to sex and age, but to tumor location, histological grade and depth of invasion. Lymph node metastasis and vascular tumor thrombus formation were related to P0.05. 98 patients with colorectal cancer. 14 of 98 patients with colorectal cancer were not treated with chemotherapy after operation. The 4-year disease-free survival rate of the remaining 84 patients was 76. 2% / 84%. The disease free survival rate of the patients with negative expression of IGF-1R protein and VEGFR2 protein was significantly higher than that of the patients with positive expression of VEGFR2 protein. The disease free survival rate of the remaining 84 patients was significantly higher than that of the patients with positive expression of 72.7% and 74. 55.The difference was statistically significant (P 0. 05). Prime Logistic regression analysis was used to analyze the sex, Age, histological grade, tumor location, depth of invasion, The expression of IGF-1R and VEGFR2 protein was still correlated with the disease free survival rate of 4 years after adjustment for lymph node metastasis and vascular tumor thrombus formation. The corrected ratio was 2.31 ~ 2.15 ~ 95% confidence interval respectively. Conclusion: colorectal cancer is 0.45% ~ 4.03. 0. 05 respectively. Conclusion: the expression of IGF-1R and VEGFR2 protein is still related to the disease free survival rate of patients with colorectal cancer. Conclusion: the expression of IGF-1R and VEGFR2 protein is still correlated with the disease free survival rate of patients with colorectal cancer. The expression of IGF-1R and VEGFR2 protein and m RNA in tumor tissues were significantly increased. The expression of protein was related to histological grade, depth of invasion, lymph node metastasis and prognosis of postoperative adjuvant chemotherapy patients.
【作者单位】：连云港市第二人民医院肿瘤化疗科;连云港市第二人民医院病理科;
【基金】：江苏大学医学临床科技发展基金项目(No.JLY20140133)
【分类号】：R735.34

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