THBS2在宫颈癌中的表达及功能研究

发布时间：2018-04-03 18:08

本文选题：宫颈癌　切入点：Thrombospondin-　出处：《现代妇产科进展》2017年12期

【摘要】：目的:探讨THBS2在宫颈癌发生发展中的表达水平变化以及THBS2对细胞增殖、迁移和侵袭能力的作用。方法:免疫组化和Western blot法检测THBS2在宫颈病变组织及细胞系中的表达水平。选取THBS2表达水平较低的宫颈癌细胞系Si Ha和ME180,通过THBS2过表达质粒上调THBS2水平。选取宫颈上皮永生化细胞株H8和ECT-1,通过转染THBS2的干扰片段siRNA下调THBS2表达。Western blot法检测THBS2表达,CCK8细胞增殖试验、划痕试验和Transwell侵袭试验观察THBS2对细胞的增殖、迁移和侵袭能力的影响。结果:THBS2在宫颈癌发生发展过程中表达水平下降(P0.001);宫颈癌细胞系Si Ha和ME180中THBS2表达水平较HPV永生化宫颈上皮细胞H8和ECT-1明显降低(P0.05)。上调宫颈癌细胞THBS2表达,Si Ha和ME180细胞的增殖(P0.05)、迁移(P0.005)和侵袭能力(P0.001)明显被抑制;下调宫颈上皮永生化细胞THBS2表达,H8和ECT-1细胞的增殖(P0.05)、迁移(P0.005)和侵袭能力(P0.001)显著增强。结论:THBS2在宫颈癌发生发展过程中可能充当"抑癌因子",是潜在的治疗靶点
[Abstract]:Aim: to investigate the expression of THBS2 in cervical carcinoma and the role of THBS2 in cell proliferation, migration and invasion.Methods: immunohistochemical and Western blot methods were used to detect the expression of THBS2 in cervical lesions and cell lines.Cervical cancer cell lines SIHA and ME180 with low THBS2 expression were selected and the THBS2 levels were up-regulated by THBS2 overexpression plasmids.Cervical epithelial immortalized cell lines H8 and ECT-1 were selected to detect the proliferation of THBS2 expressing CCK8 cells by down-regulation of THBS2 expression. Western blot, scratch test and Transwell invasion test were used to observe the proliferation of THBS2.The effect of migration and invasiveness.Results the expression of THBS2 in the cervical cancer cell line SIHA and ME180 was significantly lower than that in the HPV immortalized cervical epithelial cells H8 and ECT-1.Conclusion THBS2 may act as a "tumor suppressor" in the development of cervical cancer, and may be a potential therapeutic target.
【作者单位】：南方医科大学南方医院妇产科;南方医科大学南方医院病理科;广州医科大学附属第一医院妇产科;
【基金】：国家自然科学基金面上项目(No:81072132;81372781;81672589) 深圳市科技计划项目(No:JCYJ20160429161218745)
【分类号】：R737.33

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