K-ras对girdin蛋白表达的调控机制及二者在结直肠癌中的表达

发布时间：2018-04-10 01:18

本文选题：K-ras　切入点：Girdin　出处：《中国病理生理杂志》2017年02期

【摘要】：目的:研究K-ras对COS7细胞girdin蛋白的调控机制,并检测结直肠癌组织中k-ras和girdin的表达。方法:通过病毒载体构建稳定高表达K-ras的COS7细胞,采用Western blot方法检测稳定细胞株和结直肠癌组织中K-ras、girdin及其相关蛋白的表达。结果:高表达K-ras的COS7细胞形态不规则。Western blot实验结果显示在高表达K-ras时其下游的信号蛋白p-ERK1/2和p-Stat3的蛋白水平都明显增加,同时girdin的表达量也明显增加;当加入K-ras siRNA后,p-ERK1/2、p-Stat3和girdin的表达量也随之减少。对7例结直肠癌组织和相应癌旁组织用Western blot检测结果显示,有5例组织在K-ras高表达的同时girdin的表达量也随之增加。结论:通过细胞和组织实验结果推测K-ras蛋白通过Ras-ERK1/2-Stat3信号通路实现对girdin蛋白的调控,并为临床上与K-ras相关的肿瘤治疗提供一定的参考。
[Abstract]:Aim: to investigate the regulatory mechanism of K-ras on girdin protein in COS7 cells and to detect the expression of k-ras and girdin in colorectal carcinoma.Methods: COS7 cells with stable and high expression of K-ras were constructed by virus vector, and the expression of K-rasgirdin and its related proteins in stable cell lines and colorectal cancer tissues were detected by Western blot method.Results: the morphological changes of COS7 cells with high expression of K-ras were irregular. Western blot assay showed that the protein levels of p-ERK1/2 and p-Stat3 in the downstream of COS7 cells with high expression of K-ras were significantly increased, and the expression of girdin was also significantly increased.When K-ras siRNA was added, the expression of p-ERK 1 / 2 P -Stat3 and girdin decreased.Western blot was used to detect the expression of K-ras in 7 cases of colorectal cancer and adjacent tissues. The expression of girdin in 5 cases was increased with the high expression of K-ras.Conclusion: the results of cell and tissue experiments suggest that K-ras protein regulates girdin protein through Ras-ERK1/2-Stat3 signaling pathway and provides some reference for clinical treatment of K-ras related tumors.
【作者单位】：温州医科大学附属第二医院普外科;
【分类号】：R735.34

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