新型雌激素受体GPR30在非小细胞肺癌增殖中的作用研究

发布时间：2018-04-16 13:35

  本文选题：癌 + 非小细胞肺　； 参考：《重庆医学》2017年05期

【摘要】：目的 研究G蛋白耦联受体30(GPR30)在非小细胞肺癌(NSCLC)中的表达及其与临床主要病理特征的关系,并分析GPR30和Ki-67表达的相关性,探讨雌激素通过激活GPR30受体信号途径调节NSCLC增殖的分子机制。方法 采用免疫组织化学方法检测80例术后非小细胞肺癌组织样本中GPR30和Ki-67的表达。加入17-β-雌二醇或G-1后,计数H1299细胞,流式细胞术检测细胞周期分布,最后通过Western blot方法检测G-1作用后ERK1/2的激活状态以及cyclin D1和P16蛋白的表达。结果 GPR30更多表达在腺癌、低分化、Ⅲ期NSCLC肿瘤组织,差异有统计学意义(P0.05);GPR30表达和Ki-67呈中度相关性(r=0.502,P=0.000)。E2(或G-1)促进H1299细胞增殖,并且更多的细胞进入S期;加入G-1后,磷酸化ERK1/2以及cyclin D1表达增加,而p16蛋白表达减少,以上效应能被G-15或U0126预处理2h阻断。结论 雌激素通过激活GPR30-EGFRMAPKs信号转导途径促进H1299增殖。阻断GPR30信号途径可能成为NSCLC治疗的新靶点。
[Abstract]:Objective to study the expression of G-protein coupled receptor 30 (GPR30) in NSCLC and its relationship with the clinicopathological features, and to analyze the correlation between the expression of GPR30 and Ki-67.To explore the molecular mechanism of estrogen regulating NSCLC proliferation by activating GPR30 receptor signaling pathway.Methods Immunohistochemical method was used to detect the expression of GPR30 and Ki-67 in 80 cases of non-small cell lung cancer after operation.After adding 17- 尾 -estradiol or G-1, H1299 cells were counted, cell cycle distribution was detected by flow cytometry, and the activation state of ERK1/2 and the expression of cyclin D1 and P16 protein were detected by Western blot method.Results the expression of GPR30 in adenocarcinoma, low differentiation and stage 鈪，

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