人胎盘部位滋养细胞肿瘤细胞系的建立及其鉴定

发布时间：2018-06-04 02:46

本文选题：PSTT + 细胞系　；参考：《中国癌症杂志》2017年07期

【摘要】：背景与目的:胎盘部位滋养细胞肿瘤(placental site trophoblastic tumor,PSTT)是一种特殊类型的妊娠滋养细胞肿瘤。该研究旨在建立人PSTT细胞系,鉴定其基本特性。方法:通过手术切除的PSTT组织标本原代培养,纯化并传代,建立人PSTT细胞系。鉴定方法包括:观察细胞形态,CCK-8法绘制细胞生长曲线,进行染色体核型分析,细胞与肿瘤组织短串重复序列(short tandem repeat,STR)分析,以及免疫组织化学染色。结果:建立稳定传代的人PSTT细胞系PSTT-1。PSTT-1细胞单层贴壁生长,失去接触抑制性,呈多突起的纺锤形结构。体外培养生长良好,每5 d传代1次,目前已传至30余代。染色体核型分析结果为正常女性核型:46,XX。PSTT-1与肿瘤组织STR分析结果高度匹配。PSTT-1细胞表达β-catenin、CD146、人绒毛膜促性腺激素(human chorionic gonadotrophin,hCG)、人胎盘生乳素(human placental lactogen,hPL)和Muc4。结论:该研究成功建立人PSTT细胞系PSTT-1,为PSTT的研究提供了体外实验模型。
[Abstract]:Background & AIM: placental site trophoblastic tumor is a special type of gestational trophoblastic tumor. The aim of this study was to establish a human PSTT cell line and identify its basic characteristics. Methods: human PSTT cell line was established by primary culture, purification and passage of PSTT tissue specimen. The identification methods include: cell growth curve was drawn by observing cell morphology and CCK-8 method, karyotype analysis of chromosome, short tandem repeat analysis of cell and tumor tissue, and immunohistochemical staining. Results: the monolayer adherent growth of human PSTT cell line PSTT-1.PSTT-1 with stable passage lost contact inhibition and presented a multi-protuberant fusiform structure. The culture in vitro grew well and was subcultured once every 5 days, and has been passed to more than 30 generations. The results of chromosome karyotype analysis were as follows: 1. The karyotype of normal female was: 46-XX.PSTT-1 was highly matched with STR analysis of tumor tissue. PSTT-1 cells expressed 尾 -catenin CD146, human chorionic gonadotrophin, human placental lactogenhPLand Muc4. Conclusion: this study successfully established human PSTT cell line PSTT-1 and provided an in vitro model for the study of PSTT.
【作者单位】：复旦大学附属妇产科医院中西医结合科;上海市女性生殖内分泌相关疾病重点实验室;复旦大学附属妇产科医院病理科;复旦大学生物医学影像研究中心;
【基金】：国家自然科学基金(81472423) 上海市科委国际合作项目(14430723000)
【分类号】：R737.33

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