ZEB1及Vimentin在宫颈腺癌中的表达及临床意义

发布时间：2018-06-12 03:50

本文选题：宫颈腺癌 + ZEB1　；参考：《西南医科大学》2017年硕士论文

【摘要】：目的:观察ZEB1(zincfinger E-boxbinding homeobox1,ZEB1)和Vimentin在正常宫颈组织(NC)、宫颈原位腺癌(AIS)、宫颈腺癌(AUC)、宫颈鳞癌(SCC)及宫颈腺鳞癌(ASCC)中的表达,分析ZEB1和Vimentin在的表达及与宫颈腺癌各临床病理参数的关系,以及二者之间的相关性,探讨二者在宫颈腺癌的发生发展和侵袭转移中的作用。为宫颈腺癌的早期诊断和早期治疗提供理论依据。方法:采用Envision免疫组化法检测ZEB1和Vimentin在各研究组织中的表达情况,其中宫颈腺癌组66例,宫颈原位腺癌12例,正常宫颈组织15例;宫颈鳞癌组45例;宫颈腺鳞癌组8例。统计软件SPSS20.0用于统计描述与推断,定性资料采用频数及百分比表示,组间两两比较采用卡方检验或Fisher’s精确概率检验,线性趋势资料采用线性趋势卡方检验,ROC曲线下面积用于比较不同检查指标对宫颈癌不同病理类型的诊断价值,Spearman相关系数用于描述两个定性资料的相关性,检验水准α=0.05,当P0.05时认为差异具有统计学意义。结果:1、ZEB1在正常宫颈组中的阳性表达率为6.67%,在宫颈原位腺癌中的表达率为25.00%,在宫颈腺癌中的阳性表达率为75.76%;3组比较,阳性表达率逐步上升,差异具有统计学意义(χ2=30.147,P0.001)。其中宫颈腺癌组与正常宫颈组、宫颈原位腺癌组比较,差异显著,有统计学意义(P0.05);但正常宫颈组与宫颈原位腺癌组比较,差异无统计学意义(P0.05)。ZEB1在宫颈鳞癌中的阳性表达率为57.78%,在宫颈腺鳞癌中的阳性表达率为87.50%,与其在宫颈腺癌中的表达比较,3组差异无统计学意义(χ2=5.463,p=0.065)。2、zeb1在宫颈腺癌中的表达与患者的肿瘤浸润深度、figo分期、组织分化程度、淋巴结转移、脉管浸润及宫颈腺癌的病理亚型相关(p0.05),与肿瘤大小无关(p0.05)。3、vimentin在宫颈正常组织中无表达,在宫颈原位腺癌中的阳性表达率为16.67%,在宫颈腺癌中的阳性表达率为27.27%;3组宫颈组织病变中的表达差异具有统计学意义(χ2=8.668,p=0.013)。其中仅有原位腺癌组与正常宫颈组比较差异显著(p0.05),宫颈腺癌组与正常宫颈组、宫颈原位腺癌组比较,差异不具有统计学意义(p0.05)。vimentin在宫颈鳞癌中的阳性表达率为11.11%,在宫颈腺鳞癌中阳性表达率为75%,与宫颈腺癌的表达比较,3组差异显著,具有统计学意义(χ2=14.424,p=0.001)。宫颈腺癌组与宫颈鳞癌组、宫颈腺鳞癌组比较,差异具有统计学意义(p0.05)。4、vimentin在宫颈腺癌中的表达与患者的肿瘤figo分期、组织分化程度、淋巴结转移情况、脉管浸润有关(p0.05),与肿瘤大小、浸润深度、宫颈腺癌的病理亚型无关(p0.05)。5、在宫颈腺癌中,zeb1与vimentin的表达呈正相关且具有统计学意义(r=0.325,p0.001)。结论:1、宫颈腺癌形成过程中,伴随有zeb1、vimentin阳性表达率逐渐升高,提示zeb1、vimentin的表达可能与宫颈腺癌发生发展有关。2、在宫颈腺癌组织中zeb1和vimentin的表达随figo分期升高而增加,随组织分化级别的降低而增加;提示zeb1、vimentin可能与宫颈腺癌的恶性程度有关。在宫颈腺癌中zeb1和vimentin在有淋巴结转移组和有脉管浸润组中呈高表达,提示ZEB1、Vimentin可能与宫颈腺癌的侵袭转移有关。3、宫颈腺癌组中ZEB1的阳性表达与Vimentin的阳性表达呈正相关,提示ZEB1与Vimentin蛋白在功能上可能相关,且可能存在协同作用。
[Abstract]:Objective: To observe the expression of ZEB1 (zincfinger E-boxbinding homeobox1, ZEB1) and Vimentin in normal cervical tissue (NC), cervical adenocarcinoma in situ adenocarcinoma (AIS), cervical adenocarcinoma (AUC), cervical squamous cell carcinoma (SCC) and cervical adenosscale carcinoma (ASCC). The relationship between the expression of ZEB1 and cervical adenocarcinoma and the clinicopathological parameters of cervical adenocarcinoma and the correlation between the two were observed. To explore the role of the two in the development and invasion of cervical adenocarcinoma. To provide a theoretical basis for the early diagnosis and early treatment of cervical adenocarcinoma. Methods: Envision immunohistochemical method was used to detect the expression of ZEB1 and Vimentin in various research tissues, including 66 cases of cervical adenocarcinoma, 12 cases of cervical carcinoma in situ adenocarcinoma, and normal cervix group. 15 cases, 45 cases of cervical squamous cell carcinoma group and 8 cases of cervical adenosscale cancer group. Statistical software SPSS20.0 was used for statistical description and inference. Qualitative data were expressed in frequency and percentage, 22 were compared with chi square test or Fisher 's accurate probability test, linear trend data were tested by linear trend Chi square test, and the area under ROC curve was used for comparison. The diagnostic value for different pathological types of cervical cancer was examined. The Spearman correlation coefficient was used to describe the correlation of two qualitative data, and the level of alpha =0.05 was tested. When P0.05, the difference was statistically significant. Results: 1, the positive expression rate of ZEB1 in the normal cervical group was 6.67%, and the expression rate in the cervical carcinoma in situ was 25% in the uterine cervix. The positive expression rate in cervical adenocarcinoma was 75.76%, and the positive expression rate increased gradually in the 3 groups, and the difference was statistically significant (x 2=30.147, P0.001). The difference between the cervical adenocarcinoma group and the normal cervix group and the cervical carcinoma in situ adenocarcinoma group was significant (P0.05), but there was no statistical difference between the normal cervix group and the cervical in situ adenocarcinoma group. The positive rate of P0.05.ZEB1 in cervical squamous cell carcinoma was 57.78%, and the positive rate in cervical adenocarcinoma was 87.50%. Compared with the expression in cervical adenocarcinoma, the 3 groups had no statistical significance (x 2=5.463, p=0.065).2, ZEB1 in cervical adenocarcinoma and the depth of tumor infiltration in the patients, FIGO staging, and the degree of tissue differentiation. Lymph node metastasis, vascular infiltration and pathological subtype of cervical adenocarcinoma (P0.05) were not related to tumor size (P0.05).3, vimentin was not expressed in normal cervical tissues, the positive expression rate in cervical carcinoma in situ was 16.67%, the positive expression rate in cervical adenocarcinoma was 27.27%, and the difference in the expression of cervical tissue lesions in the 3 groups was statistically significant Significance (x 2=8.668, p=0.013). Only in situ adenocarcinoma group and normal cervical group were significantly different (P0.05), cervical adenocarcinoma group and normal cervix group, cervical in situ adenocarcinoma group, the difference was not statistically significant (P0.05) the positive rate of.Vimentin in cervical squamous cell carcinoma was 11.11%, and the positive rate in cervical adenocarcinoma was 75%, The difference between the 3 groups was significant (x 2=14.424, p=0.001). The difference between the cervical adenocarcinoma group and the cervical squamous cell carcinoma group and the cervical adenocarcinoma group was statistically significant (P0.05).4, the expression of vimentin in the cervical adenocarcinoma and the FIGO staging of the tumor, the degree of tissue differentiation, the lymph node metastasis, and the pulse tube immersion. P0.05, not related to tumor size, infiltration depth, and pathological subtype of cervical adenocarcinoma (P0.05).5. In cervical adenocarcinoma, the expression of ZEB1 and vimentin is positively correlated and has statistical significance (r=0.325, p0.001). Conclusion: 1, in the process of cervical adenocarcinoma, with ZEB1, the positive rate of vimentin increases gradually, suggesting ZEB1, vimentin table. .2 may be associated with the development of cervical adenocarcinoma. The expression of ZEB1 and vimentin in cervical adenocarcinoma increases with the increase of FIGO staging, and increases with the decrease in the grade of tissue differentiation. It suggests that ZEB1, vimentin may be associated with the malignancy of cervical adenocarcinoma. In cervical adenocarcinoma, ZEB1 and vimentin have lymph node metastasis and vascular infiltration in cervical adenocarcinoma. The high expression in the group suggests that ZEB1, Vimentin may be associated with the invasion and metastasis of cervical adenocarcinoma, and the positive expression of ZEB1 in the cervical adenocarcinoma group is positively correlated with the positive expression of Vimentin, suggesting that ZEB1 and Vimentin may be related to function and may have synergistic effect.
【学位授予单位】：西南医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R737.33

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