Cyclin Y在直肠癌中的表达及其临床意义

发布时间：2018-06-25 09:00

本文选题：直肠肿瘤 + 细胞周期蛋白Y　；参考：《青岛大学》2016年硕士论文

【摘要】：目的检测细胞周期蛋白Cyclin Y在直肠癌和正常直肠组织中的表达情况,并将其与直肠癌及多个临床病理因素进行分析,探讨Cyclin Y在直肠癌组织中的异常表达及其临床意义。方法收集烟台毓璜顶医院2014年7月~2015年6月住院行直肠癌根治术的患者共72例,所有入组患者术前均未经过手术,放化疗及生物治疗等。均行直肠癌根治术,并由同一手术团队完成。并在这72例直肠癌手术切除标本中选取30例距肿瘤5cm以上的直肠黏膜组织作为正常直肠黏膜进行对照。采用免疫组织化学法检测Cyclin Y在30例正常直肠组织、72例直肠癌组织中的表达情况。并用卡方检验、Kruskal-Wallis检验等统计学方法进行分析。结果1 Cyclin Y蛋白在直肠癌细胞中的表达定位以细胞膜和细胞质为主,细胞核中表达阴性。2直肠癌组织中Cyclin Y表达阳性率明显高于正常黏膜组织,差异具有统计学意义(χ~2=13.86,p0.05)。3不同病理分期的直肠癌患者的直肠癌组织中,Cyclin Y蛋白在的表达水平不同,差异具有统计学意义。(χ~2=7.955,p0.05)4直肠癌中Cyclin Y蛋白达阳性率在有淋巴结转移患者中明显高于无转移患者,差异有统计学意义(p0.05);不同性别、年龄、家族史、是否侵及浆膜层及肿瘤大小不同的患者直肠癌组织中的表达水平差异没有统计学意义(p0.05)。结论Cyclin Y在直肠癌中存在过表达现象,直肠癌中Cyclin Y蛋白达阳性率在有淋巴结转移患者中明显高于无转移患者,不同病理分期的直肠癌患者的直肠癌组织中Cyclin Y蛋白在的表达水平不同,可能在直肠癌发生发展过程中起重要的调节作用。
[Abstract]:Objective to detect the expression of cyclin Y in rectal cancer and normal rectal tissues, and to analyze the expression of cyclin Y in rectal cancer and other clinicopathological factors, and to explore the clinical significance of cyclin Y in rectal cancer. Methods from July 2014 to June 2015, 72 patients with rectal cancer underwent radical resection in Yantai Yuzhouding Hospital. All the patients were not operated, chemoradiotherapy and biotherapy before operation. All patients underwent radical resection of rectal cancer, which was performed by the same surgical team. 30 cases of rectal mucosa above 5cm were selected as normal rectal mucosa. Immunohistochemical method was used to detect the expression of cyclin Y in 30 normal rectal tissues (n = 72) and rectal carcinoma (n = 72). Statistical methods such as chi-square test and Kruskal-Wallis test were used. Results 1Cyclin Y protein was mainly expressed in cell membrane and cytoplasm in rectal cancer cells. The positive rate of Cyclin Y expression in nuclear negative .2 rectal cancer tissues was significantly higher than that in normal mucosal tissues. The positive rate of Cyclin Y protein in patients with lymph node metastasis was significantly higher than that in patients without lymph node metastasis (p0.05). There was no significant difference in expression levels in rectal cancer with or without invasion of serous layer and tumor size (p0.05). Conclusion there is overexpression of Cyclin Y in rectal cancer, and the positive rate of Cyclin Y protein in rectal cancer is significantly higher than that in patients with lymph node metastasis. The expression level of Cyclin Y protein in rectal cancer tissues with different pathological stages is different, which may play an important role in the progression of rectal cancer.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R735.37

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