局部高碳酸与高脂肪酸对HCC827移植瘤钙化和生长的影响

发布时间：2018-07-29 19:48

【摘要】：目的探讨局部高脂肪酸和高碳酸对HCC827人肺腺癌移植瘤钙化及生长的影响。方法选取40只直径在10~15 mm之间的HCC827人肺腺癌移植瘤裸鼠模型,随机分为4组,对照组,油酸组,碳酸氢钠(NaHCO_3)组,油酸+NaHCO_3组,每组10只,分别瘤内注射0.9%NaCl 0.2 ml,油酸0.2 ml,NaHCO_3(595 mmol/L)0.2 ml,油酸0.1 ml+NaHCO_3(595 mmol/L)0.1 ml,观察肿瘤生长的体积大小及肿瘤组织钙盐沉积情况,结果用SPSS 17.0统计软件对肿瘤体积变化进行分析,肿瘤体积大小用均数±标准差表示,多组间比较采用方差分析,各组间两两比较采用LSD-t检验。结果各组移植瘤注药后体积从小到大依次是油酸组、油酸+NaHCO_3组、NaHCO_3组和对照组,组间差异有统计学意义(F=5.773,P=0.0020.01);注药后体积差值组间差异有统计学意义(F=7.242,P=0.0010.01)。由于分组误差,所以各组在注药前体积大小不一,但差异无统计学意义(P0.05)。各组间两两比较的LSD-t检验结果显示,与对照组比较,油酸组、NaHCO_3组、油酸+NaHCO_3组注药后体积及体积差值差异均有统计学意义(注药后体积:P分别为0.001、0.006、0.002,体积差值:P分别为0、0.047、0.002);与油酸组比较,NaHCO_3组、油酸+NaHCO_3组注药后体积及体积差值差异均有统计学意义(注药后体积:P分别为0.420、0.742,体积差值:P分别为0.023、0.275);与NaHCO_3组比较,油酸+NaHCO_3组注药后体积及体积差值差异均有统计学意义(注药后体积:P为0.631,体积差值:P为0.212)。HE染色结果显示:油酸组和油酸+NaHCO_3组明显有钙盐沉积,NaHCO_3组和对照组未见明显钙盐沉积。结论局部高油酸可促进肿瘤钙盐沉积,油酸可抑制肿瘤的生长;局部高NaHCO_3可抑制肿瘤生长,但停用NaHCO_3后肿瘤体积又会继续增长,可能与NaHCO_3改变了肿瘤的偏酸性环境有关,并且油酸和NaHCO_3有协同抑制肿瘤生长的趋势。
[Abstract]:Objective to investigate the effects of local high fatty acid and high carbonic acid on calcification and growth of transplanted lung adenocarcinoma in HCC827. Methods Forty nude mice with a diameter of 10 ~ 15 mm were randomly divided into 4 groups: control group, oleic acid group, sodium bicarbonate (NaHCO_3) group and oleic acid NaHCO_3 group. 0.9%NaCl 0.2 ml, oleic acid 0.2 ml (595 mmol/L) 0.2 ml and oleic acid 0.1 ml NaHCO_3 (595 mmol/L) 0.1 ml were injected into the tumor respectively. The volume of tumor growth and calcium deposition in tumor tissue were observed. Results the changes of tumor volume were analyzed by SPSS 17.0 statistical software. The volume of tumor was expressed by mean 卤standard deviation. The analysis of variance was used to compare the tumor volume among groups and the LSD-t test was used to compare the tumor volume between two groups. Results the volume of transplanted tumor after injection was oleic acid group, oleic acid NaHCO_3 group and control group, the difference between groups was statistically significant (F5. 773, P < 0. 0020.01), and the difference of volume after injection was statistically significant (FG. 7. 242, P 0. 0010. 01). Because of the grouping error, the volume of each group was different before injection, but the difference was not statistically significant (P0.05). The results of LSD-t test showed that compared with the control group, oleic acid group was treated with NaHCO _ 3 group. There were significant differences in volume and volume difference between oleic acid NaHCO_3 group and oleic acid NaHCO_3 group. There were significant differences in volume and volume difference between NaHCO_3 group and NaHCO_3 group (volume: P was 0.420 卤0.742and volume difference was 0.023 / 0.275, respectively), compared with NaHCO_3 group, the difference of volume and volume of oleic acid NaHCO_3 group was statistically significant. There were significant differences in volume and volume difference between oleic acid NaHCO_3 group and oleic acid NaHCO_3 group (volume: P = 0.631, volume difference: 0.212). The results of HE staining showed that oleic acid group and oleic acid NaHCO_3 group obviously had calcium deposition. See obvious calcium deposit. Conclusion Local hyperoleic acid can promote tumor calcium deposition, oleic acid can inhibit tumor growth, local high NaHCO_3 can inhibit tumor growth, but tumor volume will continue to increase after discontinuation of NaHCO_3, which may be related to NaHCO_3 changing the acidic environment of tumor. Oleic acid and NaHCO_3 have the tendency of synergistic inhibition of tumor growth.
【作者单位】：山西医科大学第二医院
【分类号】：R73-3

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