IKKα及AR在前列腺癌中的表达及其临床意义
发布时间：2018-08-29 18:52
【摘要】：目的:通过观察IκB激酶α(inhibitor kappa B kinaseα,IKKα)及雄激素受体(androgen receptor,AR)在前列腺癌与前列腺增生中的表达变化,为进一步探讨其在前列腺癌发生、发展中的作用提供基础。方法:1采用免疫组化SP法检测30例良性前列腺增生组织与30例前列腺癌组织中IKKα及AR的表达。分析两者在不同组织中的表达情况、两者表达的相关性及其与前列腺癌分级、分期的关系。2采用SPSS 17.0软件对结果进行统计分析,采用卡方检验,并进行秩相关分析。检验标准以P0.05为有统计学意义。结果:1免疫组织化学结果:IKKα主要定位于腺上皮细胞的细胞浆,AR主要定位于腺上皮细胞的细胞核。IKKα在前列腺癌组中的阳性表达率为73.33%,明显高于前列腺增生组中的46.67%,差异有统计学意义(P0.05)。AR在前列腺癌组中的阳性表达率为83.33%,明显高于其在前列腺增生组中的56.67%,差异有统计学意义(P0.05)。2 IKKα及AR表达水平的相关性:在前列腺癌组中IKKα和AR的IHS评分二者的相关系数r=-0.533,有统计学意义(P0.05),说明前列腺癌组织中IKKα与AR的表达存在中等程度的负相关。3 IKKα的表达与前列腺癌分级、分期的关系:低分化前列腺癌组中IKKα的阳性表达率为93.33%,明显高于高分化组的53.33%,差异有统计学意义(P0.05)。晚期前列腺癌组中IKKα的阳性表达率为93.75%,明显高于早期组的50.00%,差异有统计学意义(P0.05)。4 AR的表达与前列腺癌分级、分期的关系:高分化前列腺癌组中AR的阳性表达率为100%,明显高于低分化组的66.67%,差异有统计学意义(P0.05)。早期前列腺癌组中AR的阳性表达率为92.86%,晚期前列腺癌组AR的阳性表达率75.00%,差异无统计学意义(P0.05)。结论:1 IKKα及AR在前列腺癌组织中的表达阳性率及强度明显高于前列腺增生组织,说明IKKα、AR与前列腺癌有密切关系,检测IKKα、AR有助于前列腺癌的早期诊断。2随着前列腺癌恶性程度增加IKKα及AR的表达分别表现出上调和下降趋势,IKKα、AR可以作为判断前列腺癌预后的指标。3 IKKα与AR的表达均与前列腺癌的生物学行为、病理密切相关,深入研究其作用机制,可能为前列腺癌的治疗提供新的思路或方法。
[Abstract]:Aim: to investigate the expression of I 魏 B kinase 伪 (inhibitor kappa B kinase 伪 Ike 伪 and androgen receptor (androgen receptor,AR) in prostate cancer and prostatic hyperplasia, and to provide a basis for further study on the role of I 魏 B kinase 伪 (inhibitor kappa B kinase 伪 in the pathogenesis and development of prostate cancer. Methods the expression of IKK 伪 and AR in 30 cases of benign prostatic hyperplasia and 30 cases of prostate cancer were detected by SP immunohistochemical method. To analyze the expression of the two in different tissues, the correlation between them and the grade and stage of prostate cancer. 2. The results were statistically analyzed by SPSS 17.0 software, chi-square test was used and rank correlation analysis was carried out. The test standard to P0.05 for statistical significance. Results the results of immunohistochemistry showed that the positive expression rate of the cytoplasmic AR of the cell nuclear of the glandular epithelial cells was 73.33%, which was significantly higher than that of 46.67% in the prostatic hyperplasia group, and the positive expression rate of K 伪 was 73.33% in the prostate cancer group, which was significantly higher than that in the prostatic hyperplasia group (46.67%). The positive expression rate of AR in prostate cancer group was 83.33, which was significantly higher than that in prostate hyperplasia group (56.67%). The difference was statistically significant (P0.05). The correlation of IKK 伪 and AR expression in prostate cancer group was significant (P0.05). The correlation coefficient between IKK 伪 and AR was significant (P 0.05), indicating that there was a moderate negative correlation between the expression of IKK 伪 and AR and the grade of prostate cancer. The positive expression rate of IKK 伪 in poorly differentiated prostate cancer group was 93.33, which was significantly higher than that in well-differentiated prostate cancer group (P 0.05). The positive expression rate of IKK 伪 in advanced prostate cancer group was 93.75, which was significantly higher than that in early prostate cancer group (P 0.05). The expression of IKK 伪 was significantly higher than that in early prostate cancer group (P0.05). The positive expression rate of AR in highly differentiated prostate cancer group was 100, which was significantly higher than that in poorly differentiated group (P 0.05). The positive expression rate of AR was 92.86 in the early prostate cancer group and 75.00 in the advanced prostate cancer group (P0.05). Conclusion the positive rate and intensity of the expression of IKK 伪 and AR in prostate cancer are significantly higher than those in benign prostatic hyperplasia, indicating that IKK 伪 AR is closely related to prostate cancer. Detection of IKK 伪 AR contributes to the early diagnosis of prostate cancer. 2 with the increase of malignant degree of prostate cancer, the expression of IKK 伪 and AR show an upward and downward trend respectively. IKK 伪 AR can be used as a prognostic indicator of prostate cancer. 3 IKK 伪 and AR expression. And the biological behavior of prostate cancer, Pathology is closely related, and further study of its mechanism may provide new ideas or methods for the treatment of prostate cancer.
【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R737.25
本文编号：2212092
[Abstract]:Aim: to investigate the expression of I 魏 B kinase 伪 (inhibitor kappa B kinase 伪 Ike 伪 and androgen receptor (androgen receptor,AR) in prostate cancer and prostatic hyperplasia, and to provide a basis for further study on the role of I 魏 B kinase 伪 (inhibitor kappa B kinase 伪 in the pathogenesis and development of prostate cancer. Methods the expression of IKK 伪 and AR in 30 cases of benign prostatic hyperplasia and 30 cases of prostate cancer were detected by SP immunohistochemical method. To analyze the expression of the two in different tissues, the correlation between them and the grade and stage of prostate cancer. 2. The results were statistically analyzed by SPSS 17.0 software, chi-square test was used and rank correlation analysis was carried out. The test standard to P0.05 for statistical significance. Results the results of immunohistochemistry showed that the positive expression rate of the cytoplasmic AR of the cell nuclear of the glandular epithelial cells was 73.33%, which was significantly higher than that of 46.67% in the prostatic hyperplasia group, and the positive expression rate of K 伪 was 73.33% in the prostate cancer group, which was significantly higher than that in the prostatic hyperplasia group (46.67%). The positive expression rate of AR in prostate cancer group was 83.33, which was significantly higher than that in prostate hyperplasia group (56.67%). The difference was statistically significant (P0.05). The correlation of IKK 伪 and AR expression in prostate cancer group was significant (P0.05). The correlation coefficient between IKK 伪 and AR was significant (P 0.05), indicating that there was a moderate negative correlation between the expression of IKK 伪 and AR and the grade of prostate cancer. The positive expression rate of IKK 伪 in poorly differentiated prostate cancer group was 93.33, which was significantly higher than that in well-differentiated prostate cancer group (P 0.05). The positive expression rate of IKK 伪 in advanced prostate cancer group was 93.75, which was significantly higher than that in early prostate cancer group (P 0.05). The expression of IKK 伪 was significantly higher than that in early prostate cancer group (P0.05). The positive expression rate of AR in highly differentiated prostate cancer group was 100, which was significantly higher than that in poorly differentiated group (P 0.05). The positive expression rate of AR was 92.86 in the early prostate cancer group and 75.00 in the advanced prostate cancer group (P0.05). Conclusion the positive rate and intensity of the expression of IKK 伪 and AR in prostate cancer are significantly higher than those in benign prostatic hyperplasia, indicating that IKK 伪 AR is closely related to prostate cancer. Detection of IKK 伪 AR contributes to the early diagnosis of prostate cancer. 2 with the increase of malignant degree of prostate cancer, the expression of IKK 伪 and AR show an upward and downward trend respectively. IKK 伪 AR can be used as a prognostic indicator of prostate cancer. 3 IKK 伪 and AR expression. And the biological behavior of prostate cancer, Pathology is closely related, and further study of its mechanism may provide new ideas or methods for the treatment of prostate cancer.
【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R737.25
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