NOX通路在氧化应激诱导的宫颈癌细胞凋亡中的作用

发布时间：2018-10-05 08:55

【摘要】：目的探讨烟酰胺腺嘌呤二核苷酸磷酸氧化酶(NOX)通路在氧化应激诱导的宫颈癌细胞凋亡中的作用。方法流式细胞术检测不同类型宫颈癌细胞内在的总活性氧(ROS)水平及NOX途径抑制剂DPI处理前后不同类型宫颈癌细胞内线粒体途径和NOX途径ROS水平;DPI预处理、H_2O_2作用后采用流式细胞术检测不同类型宫颈癌细胞凋亡率。结果人乳头瘤病毒(HPV)阳性宫颈癌细胞内在ROS水平明显高于HPV阴性宫颈癌细胞(P0.05),两类宫颈癌细胞线粒体途径来源ROS水平比较差异无统计学意义(P0.05)。与预处理前比较,DPI预处理后HPV阳性宫颈癌细胞内在ROS水平均明显降低(P均0.05)。与诱导前及HPV阴性宫颈癌细胞比较,H_2O_2诱导后HPV阳性宫颈癌细胞凋亡率均明显升高,且高于DPI预处理及DPI预处理+H_2O_2诱导细胞(P均0.05)。结论 NOX通路在HPV感染导致宫颈癌的过程中具有重要作用,促氧化治疗可能更好地杀伤HPV阳性宫颈癌细胞。
[Abstract]:Objective to investigate the role of nicotinamide adenine dinucleotide phosphate oxidase (NOX) pathway in oxidative stress induced apoptosis of cervical cancer cells. Methods flow cytometry was used to detect the intrinsic levels of total reactive oxygen species (ROS) in different types of cervical cancer cells and NOX pathway inhibitor (DPI) before and after treatment with different types of cervical cancer cells. The mitochondrial pathway and NOX pathway ROS level of different types of cervical cancer cells were pretreated with H _ 2O _ 2. Apoptosis rate of different types of cervical cancer cells was detected by flow cytometry. Results the ROS level of (HPV) positive cervical cancer cells was significantly higher than that of HPV negative cervical cancer cells (P0.05). There was no significant difference in ROS level between the two types of cervical cancer cells derived from mitochondrial pathway (P0.05). Compared with that before preconditioning, the ROS level of HPV positive cervical cancer cells was significantly lower than that of pretreated cells (P < 0. 05). The apoptosis rate of HPV positive cervical cancer cells was significantly higher than that of DPI pretreatment and DPI pretreatment (P < 0. 05). Conclusion NOX pathway plays an important role in the process of cervix cancer caused by HPV infection, and it may be better to kill HPV positive cervical cancer cells by the treatment of promoting oxidation.
【作者单位】：安徽医科大学附属省立医院;蚌埠医学院;
【基金】：安徽高校自然科学基金重点项目(KJ2016A472)
【分类号】：R737.33

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