DIAPH3蛋白对三阴性乳腺癌细胞增殖和迁移侵袭能力的影响

发布时间：2018-10-08 14:33

【摘要】：目的:探讨DIAPH3蛋白对三阴性乳腺癌细胞迁移侵袭能力的影响;方法:本研究通过q PCR和western blot探讨DIAPH3蛋白在MCF-10A、SUM159、MCF-7和MDA-MB-231细胞中的表达,然后通过构建过表达载体过表达DIAPH3,通过Transwell分析过表达DIAPH3蛋白对三阴性乳腺癌细胞迁移和侵袭能力的影响,最后探讨DIAPH3蛋白影响三阴性乳腺癌细胞迁移和侵袭能力的分子机制。结果:MCF-10A、SUM159、MCF-7和MDA-MB-231中的相对表达量依次为1.00±0.000、0.47±0.068、0.64±0.039和0.17±0.008。和正常乳腺上皮细胞MCF-10A相比,乳腺癌细胞SUM159、MCF-7和MDA-MB-231中DIAPH3蛋白的相对表达量显著降低,差异具有统计学意义(P0.05)。和MCF-7相比,SUM159和MDA-MB-231中DIAPH3蛋白的相对表达量显著降低,差异具有统计学意义(P0.05)。Cell group、NC group和DIAPH3 group中MDA-MB-231细胞的迁移数目分别为305.5±6.36、329±16.97和221±24.04;Cell group、NC group和DIAPH3 group中MDA-MB-231细胞的侵袭数目为96±8.25、103±18.59和24.5±5.68;和Cell group、NC group相比,过表达DIAPH3能够显著抑制MDA-MB-231细胞的迁移和侵袭;进一步研究表明过表达DIAPH3蛋白能够抑制Rho A、MMP-2和MMP-9蛋白的表达。结论:DIAPH-3在乳腺癌细胞中表达显著降低,在三阴性乳腺癌细胞中表达进一步降低;过表达DIAPH-3能够抑制Rho A、MMP-2和MMP-9蛋白的表达,从而抑制TNBC细胞的迁移和侵袭。
[Abstract]:Objective: to investigate the effect of DIAPH3 protein on the migration and invasion of triple-negative breast cancer cells. Methods: to investigate the expression of DIAPH3 protein in MCF-10A,SUM159,MCF-7 and MDA-MB-231 cells by Q PCR and western blot. Then the overexpression vector DIAPH3, was constructed to analyze the effect of overexpression of DIAPH3 protein on the migration and invasion of triple-negative breast cancer cells by Transwell. Finally, the molecular mechanism of DIAPH3 protein affecting the migration and invasion of triple-negative breast cancer cells was discussed. Results the relative expression levels in MCF-7 and MDA-MB-231 were 1.00 卤0.000,0.47 卤0.068,0.64 卤0.039 and 0.17 卤0.008, respectively. Compared with normal breast epithelial cell MCF-10A, the relative expression of DIAPH3 protein in SUM159,MCF-7 and MDA-MB-231 of breast cancer cells was significantly lower than that of normal breast epithelial cells (P0.05). The relative expression of DIAPH3 protein in SUM159 and MDA-MB-231 was significantly lower than that in MCF-7 (P0.05). The number of migration of MDA-MB-231 cells in group,NC group and DIAPH3 group was 305.5 卤6.36329 卤16.97 and 221 卤24.04Cell group,NC group, respectively, and the number of invasion of MDA-MB-231 cells in DIAPH3 group was 96 卤8.25103 卤18.59 and 24.5 卤5.68, respectively, compared with Cell group,NC group. Overexpression of DIAPH3 could significantly inhibit the migration and invasion of MDA-MB-231 cells, and further studies showed that overexpression of DIAPH3 protein could inhibit the expression of Rho Agna MMP-2 and MMP-9 proteins. Conclusion the expression of DIAPH-3 in breast cancer cells was significantly lower than that in triple-negative breast cancer cells, and the overexpression of DIAPH-3 could inhibit the expression of MMP-2 and MMP-9 proteins in TNBC cells, thus inhibiting the migration and invasion of TNBC cells.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R737.9

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