胆管细胞癌中CtBP1、Zeb1、Zeb2与E-cadherin蛋白的表达及临床意义

发布时间：2018-11-07 12:31

【摘要】：目的探讨转录抑制因子Zeb1、Zeb2、转录辅抑制因子CtBP1及其靶基因E-cadherin在胆管细胞癌中的表达及CtBP1、Ecadherin的临床意义。方法采用免疫组化法检测胆管细胞癌及癌旁组织芯片中CtBP1、Zeb1、Zeb2和E-cadherin的表达。结果CtBP1在胆管细胞癌及癌旁组织中的阳性率分别为44.44%和17.86%,Zeb2分别为34.92%和10.71%,E-cadherin分别为50.79%和100%,组间差异均有统计学意义(P均0.05)。Zeb1在胆管细胞癌仅1例表达,而在癌旁组织中不表达。CtBP1与胆管细胞癌的分化程度相关(P0.05),E-cadherin与胆管细胞癌的分化程度及远处转移有关(P均0.05)。E-cadherin和CtBP1及Zeb2表达呈负相关(r=-0.034、-0.029,P均0.001),CtBP1和Zeb2表达呈正相关(r=0.228,P=0.005)。结论胆管细胞癌中CtBP1、Zeb2与E-cadherin均表达异常,CtBP1、Zeb2可能共同参与E-cadherin表达调控。联合检测CtBP1和E-cadherin有望成为评估胆管细胞癌恶性生物学行为的参考指标。
[Abstract]:Objective to investigate the expression of Zeb1,Zeb2, transcription coinhibitor CtBP1 and its target gene E-cadherin in cholangiocarcinoma and the clinical significance of CtBP1,Ecadherin. Methods Immunohistochemical method was used to detect the expression of CtBP1,Zeb1,Zeb2 and E-cadherin in cholangiocarcinoma and adjacent tissue microarray. Results the positive rates of CtBP1 in cholangiocarcinoma and adjacent tissues were 44.44% and 17.86%, respectively. The positive rates of Zeb2 and E-cadherin were 34.92% and 10.71%, 50.79% and 100%, respectively. Zeb1 was expressed in only 1 case of cholangiocarcinoma, but not in adjacent tissues. CtBP1 was correlated with the degree of differentiation of cholangiocarcinoma (P0.05). E-cadherin was correlated with the differentiation and distant metastasis of cholangiocarcinoma (P0. 05). The expression of E-cadherin, CtBP1 and Zeb2 were negatively correlated (r = 0. 034-0. 029 P = 0.001), and the expression of CtBP1 and Zeb2 were positively correlated (r = 0. 228, P < 0. 05). P0. 005). Conclusion both CtBP1,Zeb2 and E-cadherin are abnormal in cholangiocarcinoma, and CtBP1,Zeb2 may be involved in the regulation of E-cadherin expression. Combined detection of CtBP1 and E-cadherin is expected to be a reference index for evaluating malignant biological behavior of cholangiocarcinoma.
【作者单位】：桂林医学院附属医院病理科;桂林医学院研究生院;桂林医学院基础医学院生理学教研室;桂林医学院第二附属医院病理科;
【基金】：国家自然科学基金(81660485) 广西壮族自治区和计划生育委员会资助课题(Z2015404)
【分类号】：R735.8

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