芒柄花黄素对人胃癌细胞株MKN-45增殖、凋亡的影响及其机制

发布时间：2018-11-08 19:31

【摘要】：目的观察芒柄花黄素对人胃癌MKN-45细胞株增殖、凋亡的影响,并探讨其可能作用机制。方法取对数生长期人胃癌MKN-45细胞分为A、B、C、对照组,A、B、C组分别加入20、40、80μg/m L芒柄花黄素培养,对照组加入不含芒柄花黄素的完全培养基。采用MTT法观察给药24、48、72 h各组细胞增殖情况,计算细胞增殖抑制率。采用DAPI染色法评价其对MKN-45细胞形态学的影响。采用Western blotting法检测给药48 h时各组细胞磷酸化IκB(p-IκB)及NF-κB p65。结果随芒柄花黄素浓度升高,细胞增殖抑制率升高,且随干预时间增加,细胞增殖抑制率升高(P均0.05)。给药72 h时A、B、C、对照组的凋亡率分别为25.62%±2.57%、48.27%±3.18%、72.51%±4.35%、1.07%±0.54%,A、B、C组与对照组相比,P均0.05。给药48 h时A、B、C组细胞p-IκB、NF-κB p65蛋白相对表达量均高于对照组,且随芒柄花黄素浓度升高,细胞p-IκB及NF-κB p65蛋白相对表达量增加,P均0.05。结论芒柄花黄素可抑制人胃癌MKN-45细胞株的增殖、促进细胞凋亡,其机制可能为芒柄花黄素激活NF-κB信号通路。
[Abstract]:Objective to observe the effect of Flavatin on proliferation and apoptosis of human gastric cancer MKN-45 cell line and to explore its possible mechanism. Methods Human gastric cancer MKN-45 cells at logarithmic growth stage were divided into two groups: control group and control group. The control group was cultured with 20 ~ 4080 渭 g / m ~ (-1) amaranthin, and the control group was cultured on a complete medium without Acanthoxanthin. MTT assay was used to observe the proliferation of the cells in each group for 72 h. The effect of DAPI staining on the morphology of MKN-45 cells was evaluated. Phosphorylated I 魏 B (p-I 魏 B) and NF- 魏 B p65 were detected by Western blotting assay at 48 h after administration. Results the inhibition rate of cell proliferation was increased with the increase of the concentration of anthocyanin, and the inhibition rate of cell proliferation was increased with the increase of intervention time (P 0.05). At 72 h after administration, the apoptotic rates in the control group were 25.62% 卤2.57% 卤48.27% 卤3.18%, respectively, and 72.551% 卤4.3551% 卤0.54% in the control group, respectively, compared with those in the control group (P 0.05). The relative expression of p-I 魏 B and NF- 魏 B p65 protein in the cells of Agni BU C group was higher than that in the control group at 48 h, and the relative expression of p-I 魏 B protein and NF- 魏 B p65 protein increased with the increase of Flavatin concentration (P 0.05). Conclusion Acanthopanthin can inhibit the proliferation of human gastric cancer MKN-45 cell line and promote apoptosis. The mechanism may be the activation of NF- 魏 B signaling pathway by Acanthopanthin.
【作者单位】：广西壮族自治区人民医院;桂林医学院附属医院;
【基金】：广西高校科学技术研究项目(LX2014270)
【分类号】：R735.2

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