MicroRNA-223表达与肝癌根治性切除术预后的相关性

发布时间：2018-11-25 10:33

【摘要】：目的识别可以作为肝癌根治性切除术患者预后标志物的microRNA(miRNA)分子。方法利用miRNA微阵列方法对2例原发性肝癌及对应的肺转移性肝癌进行miRNA表达分析。将原发性和转移性肝癌中表达量有2倍差异的miRNAs作为候选miRNAs分子。在miRNA验证过程中,运用实时荧光定量聚合酶链反应(qRT-PCR)分析37例肝癌及相应癌旁非肿瘤组织中候选miRNAs分子的表达,并将其与患者的临床病理特征及生存情况进行相关性分析。结果最终选取8种miRNA(smiR-27b、122、142-5p、196a、223、590-5p、630及944)作为候选miRNA。只有miR-223表达水平与肿瘤分期、有丝分裂指数相关。将肝癌患者分为miR-223高表达组(25例)和miR-223低表达组(12例)后发现,miR-223高表达与较高T分期、淋巴结转移、较高丝分裂指数及较高Ki-67阳性指数相关。此外,miR-223高表达还与总体生存率和无病存活率下降相关(P0.05),中位随访时间37.9个月[疾病复发危险比为16.267(95%CI:1.732,153.789)P=0.015]。结论肝癌组织中miR-223异常表达与肝癌患者治疗结束后发生复发转移,以及无病生存时间和总生存率有关,通过检测肿瘤组织中miR-223 qRT-PCR反应有可能预测患者预后。
[Abstract]:Objective to identify the microRNA (miRNA) molecules that can be used as prognostic markers in patients with hepatocellular carcinoma after radical resection. Methods miRNA microarray method was used to analyze the expression of miRNA in 2 cases of primary liver cancer and corresponding metastatic lung cancer. MiRNAs, which is twice different in expression between primary and metastatic hepatocellular carcinoma, was used as candidate miRNAs molecule. In the process of miRNA validation, the expression of candidate miRNAs molecules in 37 cases of HCC and its adjacent non-tumor tissues was analyzed by real-time fluorescence quantitative polymerase chain reaction (qRT-PCR). The correlation analysis was made with the clinicopathological features and survival conditions of the patients. Results eight kinds of miRNA (smiR-27b,122142-5p,196a,223590-5p,630 and 944) were selected as candidate miRNA.. Only the level of miR-223 expression was correlated with tumor stage and mitotic index. The high expression of miR-223 was found to be associated with higher T stage, lymph node metastasis, higher mitotic index and higher Ki-67 positive index after being divided into miR-223 high expression group (25 cases) and low miR-223 expression group (12 cases). In addition, the high expression of miR-223 was also associated with the decrease of overall survival rate and disease-free survival rate (P0.05), with a median follow-up time of 37.9 months [disease recurrence risk ratio was 16.267 (95%CI:1.732153.789) 0.015]. Conclusion the abnormal expression of miR-223 in HCC tissues is related to recurrence and metastasis, disease-free survival time and overall survival rate of HCC patients. Detection of miR-223 qRT-PCR reaction in HCC tissues may predict the prognosis of HCC patients.
【作者单位】：武汉理工大学校医院;南华大学附属第二医院;
【分类号】：R735.7

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