Lrig1基因在胃癌组织和外周血中的表达及其与肿瘤标志物的联合检测
发布时间：2018-11-26 09:02
【摘要】：目的探讨Lrig1、EGFR在人体胃癌组织和外周血中的表达情况和临床意义,以及Lrig1与肿瘤标记物CEA、CA724的联合检测对胃癌的诊断价值分析。方法采用免疫组化二步法检测Lrig1和EGFR蛋白在57例胃癌组织和相应癌旁正常胃组织中的表达情况,分析其与临床病理参数的相关性;通过RT-PCR检测Lri g1和EGFR m RNA在57例胃癌组织中和相应癌旁正常胃组织的相对表达量,以及L rig1 m RNA在57例胃癌患者和健康人外周血中的相对表达量;通过电化学发光法检测57例胃癌患者及健康人血清中CEA、CA724的表达水平,并联合Lrig1 m RNA在外周血中的表达,对结果进行分析。结果1、Lrig1在胃癌组织中的阳性表达率(38.6%)低于正常胃组织(82.5%),而E GFR在胃癌组织中的阳性表达率(63.2%)显高于正常胃组织(15.8%),差异具有统计学意义(P0.05)。Lrig1和EGFR蛋白在胃癌组织中的表达水平呈显著负相关(r=-0.515,P0.01)。Lrig1蛋白的表达与胃癌患者的性别、年龄、肿瘤大小、分化程度、TNM分期和有无淋巴结转移无关(P0.05),EGFR蛋白表达与患者年龄、性别、肿瘤大小无关(P0.05),而与肿瘤分化程度、TNM分期和有无淋巴结转移密切相关(P0.01)。2、胃癌组织中Lrig1 m RNA表达水平(0.43±0.16)较相应癌旁正常组织(1.06±0.21)降低,而EGFR m RNA表达水平(3.27±0.67)高于相应癌旁正常组织(1.33±0.28),P0.05。Lrig1和EGFR m RNA在胃癌组织中的表达水平呈显著负相关(r=-0.874,P0.01)。3、胃癌患者静脉血中Lrig1 m RNA表达水平(0.73±0.21)较健康对照组(1.28±0.27)降低(P0.05)。4、胃癌患者血清CEA(39.6±16.5)、CA724(58.8±19.6)水平明显高于对照组CEA(3.3±1.2)、CA724(4.5±1.7),P0.05,且胃癌患者血清中CE A(54.4%)、CA724(73.7%)阳性率明显高于对照组CEA(14.0%)、CA724(8.8%),P0.05,胃癌患者静脉血中Lrig1 m RNA的表达阳性率(49.1%)明显低于对照组(87.7%),P0.05。Lrig1和CEA、CA724在胃癌患者静脉血中和健康对照组静脉血中的表达水平呈负相关(r=-0.509,P0.01;r=-0.369,P0.01)。Lrig1和CEA、CA724三者联合检验的敏感性和特异性高于单一检测的结果。结论1、Lrig1在胃癌组织中存在低表达,提示Lrig1在参与胃癌的发生发展过程中可能起着抑癌基因的作用,其表达并不能决定胃癌的病理分型;EGFR在胃癌组织中的高表达提示EGFR可能在胃癌的发病机制中和生长增殖中发挥关键作用。2、胃癌组织中Lrig1与EGFR的表达呈负相关,提示Lrig1可能存在对EGFR的负反馈调节,二者在正常情况下保持一定的平衡。3、肿瘤标志物CEA和CA724对胃癌均有一定的敏感性和特异性,Lrig1 m R NA与其联合检测提高了对胃癌诊断的敏感性和特异性,这对胃癌的早期防治和跟踪治疗有很大的参考价值。
[Abstract]:Objective to investigate the expression and clinical significance of Lrig1,EGFR in human gastric cancer tissues and peripheral blood, and to analyze the diagnostic value of Lrig1 combined with tumor marker CEA,CA724 in gastric cancer. Methods Immunohistochemical two-step method was used to detect the expression of Lrig1 and EGFR protein in 57 cases of gastric cancer and adjacent normal gastric tissues, and the correlation between the expression and clinicopathological parameters was analyzed. The relative expression of Lri G1 and EGFR m RNA in 57 cases of gastric cancer and the corresponding normal gastric tissues were detected by RT-PCR, and the relative expression of L rig1 m RNA in peripheral blood of 57 cases of gastric cancer and healthy persons was also detected. The expression of CEA,CA724 in serum of 57 patients with gastric cancer and healthy persons was detected by electrochemiluminescence, and the expression of Lrig1 m RNA in peripheral blood was analyzed. Results 1the positive expression rate of Lrig1 in gastric cancer tissues (38.6%) was lower than that in normal gastric tissues (82.5%), while the positive expression rate of E GFR in gastric cancer tissues (63.2%) was significantly higher than that in normal gastric tissues (15.8%). The difference was statistically significant (P0.05). There was a significant negative correlation between the expression of Lrig1 and EGFR in gastric cancer tissues (r-0.515, P0.01). The expression of Lrig1 protein was correlated with the sex, age, tumor size, differentiation degree of gastric cancer patients. TNM stage was not related to lymph node metastasis (P0.05), EGFR protein expression was not related to age, sex and tumor size (P0.05), but was closely related to tumor differentiation, TNM stage and lymph node metastasis (P0.01). The expression of Lrig1 m RNA in gastric cancer (0.43 卤0.16) was significantly lower than that in adjacent normal tissues (1.06 卤0.21), while the expression of EGFR m RNA was (3.27 卤0.67) higher than that in adjacent normal tissues (1.33 卤0.28). The expression of P0.05.Lrig1 and EGFR m RNA in gastric carcinoma was negatively correlated (r = -0.874, P 0.01). The expression of Lrig1 m RNA in the venous blood of gastric cancer patients (0.73 卤0.21) was significantly lower than that in the healthy controls (1.28 卤0.27) (P0.05), and the serum CEA level in gastric cancer patients was (39.6 卤16.5). The levels of CA724 (58.8 卤19.6) were significantly higher than those of control group (3.3 卤1.2), CA724 (4.5 卤1.7), P0.05, and the serum CE A (of gastric cancer patients was 54.4%. The positive rate of CA724 (73.7%) was significantly higher than that of control group (14.0%), CA724 (8.8%), P0.05. The positive rate of Lrig1 m RNA expression in venous blood of gastric cancer patients (49.1%) was significantly lower than that of control group (87.7%). There was a negative correlation between the expression of P0.05.Lrig1 and CEA,CA724 in the venous blood of gastric cancer patients and the healthy control group (r = -0.509, P 0.01). The sensitivity and specificity of Lrig1 and CEA,CA724 combined detection were higher than that of single detection. Conclusion 1 the low expression of Lrig1 in gastric carcinoma suggests that Lrig1 may play an important role in the carcinogenesis and development of gastric cancer, and its expression can not determine the pathological type of gastric cancer. The high expression of EGFR in gastric cancer suggests that EGFR may play a key role in the pathogenesis and growth of gastric cancer. 2There is a negative correlation between Lrig1 and EGFR expression in gastric cancer, suggesting that Lrig1 may have negative feedback regulation on EGFR. The tumor markers CEA and CA724 have a certain sensitivity and specificity for gastric cancer. The combined detection of Lrig1 m R NA and CA724 improves the sensitivity and specificity of the diagnosis of gastric cancer. It has great reference value for early prevention and treatment of gastric cancer.
【学位授予单位】：辽宁医学院
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R735.2
本文编号：2358073
[Abstract]:Objective to investigate the expression and clinical significance of Lrig1,EGFR in human gastric cancer tissues and peripheral blood, and to analyze the diagnostic value of Lrig1 combined with tumor marker CEA,CA724 in gastric cancer. Methods Immunohistochemical two-step method was used to detect the expression of Lrig1 and EGFR protein in 57 cases of gastric cancer and adjacent normal gastric tissues, and the correlation between the expression and clinicopathological parameters was analyzed. The relative expression of Lri G1 and EGFR m RNA in 57 cases of gastric cancer and the corresponding normal gastric tissues were detected by RT-PCR, and the relative expression of L rig1 m RNA in peripheral blood of 57 cases of gastric cancer and healthy persons was also detected. The expression of CEA,CA724 in serum of 57 patients with gastric cancer and healthy persons was detected by electrochemiluminescence, and the expression of Lrig1 m RNA in peripheral blood was analyzed. Results 1the positive expression rate of Lrig1 in gastric cancer tissues (38.6%) was lower than that in normal gastric tissues (82.5%), while the positive expression rate of E GFR in gastric cancer tissues (63.2%) was significantly higher than that in normal gastric tissues (15.8%). The difference was statistically significant (P0.05). There was a significant negative correlation between the expression of Lrig1 and EGFR in gastric cancer tissues (r-0.515, P0.01). The expression of Lrig1 protein was correlated with the sex, age, tumor size, differentiation degree of gastric cancer patients. TNM stage was not related to lymph node metastasis (P0.05), EGFR protein expression was not related to age, sex and tumor size (P0.05), but was closely related to tumor differentiation, TNM stage and lymph node metastasis (P0.01). The expression of Lrig1 m RNA in gastric cancer (0.43 卤0.16) was significantly lower than that in adjacent normal tissues (1.06 卤0.21), while the expression of EGFR m RNA was (3.27 卤0.67) higher than that in adjacent normal tissues (1.33 卤0.28). The expression of P0.05.Lrig1 and EGFR m RNA in gastric carcinoma was negatively correlated (r = -0.874, P 0.01). The expression of Lrig1 m RNA in the venous blood of gastric cancer patients (0.73 卤0.21) was significantly lower than that in the healthy controls (1.28 卤0.27) (P0.05), and the serum CEA level in gastric cancer patients was (39.6 卤16.5). The levels of CA724 (58.8 卤19.6) were significantly higher than those of control group (3.3 卤1.2), CA724 (4.5 卤1.7), P0.05, and the serum CE A (of gastric cancer patients was 54.4%. The positive rate of CA724 (73.7%) was significantly higher than that of control group (14.0%), CA724 (8.8%), P0.05. The positive rate of Lrig1 m RNA expression in venous blood of gastric cancer patients (49.1%) was significantly lower than that of control group (87.7%). There was a negative correlation between the expression of P0.05.Lrig1 and CEA,CA724 in the venous blood of gastric cancer patients and the healthy control group (r = -0.509, P 0.01). The sensitivity and specificity of Lrig1 and CEA,CA724 combined detection were higher than that of single detection. Conclusion 1 the low expression of Lrig1 in gastric carcinoma suggests that Lrig1 may play an important role in the carcinogenesis and development of gastric cancer, and its expression can not determine the pathological type of gastric cancer. The high expression of EGFR in gastric cancer suggests that EGFR may play a key role in the pathogenesis and growth of gastric cancer. 2There is a negative correlation between Lrig1 and EGFR expression in gastric cancer, suggesting that Lrig1 may have negative feedback regulation on EGFR. The tumor markers CEA and CA724 have a certain sensitivity and specificity for gastric cancer. The combined detection of Lrig1 m R NA and CA724 improves the sensitivity and specificity of the diagnosis of gastric cancer. It has great reference value for early prevention and treatment of gastric cancer.
【学位授予单位】：辽宁医学院
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R735.2
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