泛素C端水解酶37通过去泛素化巨噬细胞迁移抑制因子促进结肠癌细胞增殖
发布时间：2019-01-05 15:12
【摘要】：目的检测UCH37在结肠癌中的表达,并分析其与患者的临床病理特征的相关性,及其对结肠癌细胞增殖和凋亡的影响及分子机制。方法利用免疫组化检测结肠癌组织中UCH37蛋白的表达情况,分析UCH37与结肠癌患者临床病理特征的相关性,qPCR、CCK-8、流式细胞术和免疫共沉淀检测UCH37对结肠癌细胞增殖、凋亡及MIF的去泛素化水平的影响。结果 UCH37蛋白在结肠癌组织中的阳性率高于癌旁组织,其表达水平与患者TNM分期及核分化水平呈正相关;结肠癌细胞中UCH37的表达水平显著高于正常结肠内皮细胞;转染UCH37 siRNA组细胞增殖能力低于转染NC组,而凋亡细胞比例高于转染NC组;感染UCH37慢病毒组增殖能力高于感染空载体组,而凋亡细胞比例低于感染空载体组。其机制为UCH37在结肠癌细胞中去泛素化MIF。结论 UCH37在结肠癌中表达升高,并通过去泛素化MIF促进结肠癌细胞增殖。
[Abstract]:Objective to detect the expression of UCH37 in colon cancer and analyze its correlation with the clinicopathological features of colon cancer and its effect on the proliferation and apoptosis of colon cancer cells and its molecular mechanism. Methods the expression of UCH37 protein in colon cancer tissues was detected by immunohistochemistry, and the correlation between UCH37 and clinicopathological features of colon cancer patients was analyzed. QPCR,CCK-8, flow cytometry and co-immunoprecipitation were used to detect the proliferation of colon cancer cells by UCH37. Apoptosis and the effect of MIF deoxyuridine level. Results the positive rate of UCH37 protein in colon cancer tissue was higher than that in paracancerous tissue, and its expression level was positively correlated with TNM stage and nuclear differentiation level, and the expression level of UCH37 in colon cancer cell was significantly higher than that in normal colon endothelial cell. The proliferative ability of transfected UCH37 siRNA group was lower than that of transfected NC group, while the proportion of apoptotic cells was higher in infected UCH37 lentivirus group than that in infected empty vector group, while the proportion of apoptotic cells in infected UCH37 lentivirus group was lower than that in empty vector group. The mechanism is that UCH37 deubiquitin MIF. in colon cancer cells. Conclusion the expression of UCH37 in colon cancer is increased and the proliferation of colon cancer cells is promoted by MIF.
【作者单位】： 西南医科大学附属医院消化内科;绵阳市中心医院消化内科;
【分类号】：R735.35
[Abstract]:Objective to detect the expression of UCH37 in colon cancer and analyze its correlation with the clinicopathological features of colon cancer and its effect on the proliferation and apoptosis of colon cancer cells and its molecular mechanism. Methods the expression of UCH37 protein in colon cancer tissues was detected by immunohistochemistry, and the correlation between UCH37 and clinicopathological features of colon cancer patients was analyzed. QPCR,CCK-8, flow cytometry and co-immunoprecipitation were used to detect the proliferation of colon cancer cells by UCH37. Apoptosis and the effect of MIF deoxyuridine level. Results the positive rate of UCH37 protein in colon cancer tissue was higher than that in paracancerous tissue, and its expression level was positively correlated with TNM stage and nuclear differentiation level, and the expression level of UCH37 in colon cancer cell was significantly higher than that in normal colon endothelial cell. The proliferative ability of transfected UCH37 siRNA group was lower than that of transfected NC group, while the proportion of apoptotic cells was higher in infected UCH37 lentivirus group than that in infected empty vector group, while the proportion of apoptotic cells in infected UCH37 lentivirus group was lower than that in empty vector group. The mechanism is that UCH37 deubiquitin MIF. in colon cancer cells. Conclusion the expression of UCH37 in colon cancer is increased and the proliferation of colon cancer cells is promoted by MIF.
【作者单位】： 西南医科大学附属医院消化内科;绵阳市中心医院消化内科;
【分类号】：R735.35
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