TRIM28在结直肠癌组织中的表达及其对癌细胞增殖、迁移能力的影响

发布时间：2019-01-17 07:19

【摘要】：目的观察三结构域蛋白家族(TRIM)28在结直肠癌组织中的表达情况及其对癌细胞增殖、迁移能力的影响。方法采用免疫组化法检测145例结直肠癌及其相应癌旁组织中的TRIM28,分析其与结直肠癌患者临床病理特征及生存预后的关系。将结直肠癌HT29细胞随机分为观察组和对照组,分别转染sh TRIM28降表达质粒、空载对照质粒48 h,采用Western blot法检测HT29细胞中的TRIM28,分别采用MTT法和细胞划痕实验观察HT29细胞的增殖及迁移能力。结果结直肠癌组织中TRIM28高表达97例,高于癌旁组织中的4例(P0.01);并且,存在淋巴结转移、远处转移及Dukes分期高者TRIM28表达水平高于无淋巴结转移和远处转移及Dukes分期低者(P均0.05)。TRIM28高表达者3年无病生存率为68.0%,低于TRIM28低表达者的83.3%(P0.01);COX比例风险模型多因素分析结果显示,TRIM28表达是结直肠癌患者独立的预后因素(HR=3.26,P=0.01)。观察组HT29细胞中TRIM28相对表达量低于对照组(P0.01),且观察组细胞增殖OD值及迁移距离均小于对照组(P均0.01)。结论 TRIM28在结直肠癌组织中高表达,降低其表达可抑制结直肠癌细胞的增殖及迁移;其在结直肠癌的发生发展中起重要作用,可作为结直肠癌患者的预后指标和潜在治疗靶点。
[Abstract]:Objective to investigate the expression of (TRIM) 28 in colorectal carcinoma and its effect on the proliferation and migration of cancer cells. Methods Immunohistochemical method was used to detect the relationship between TRIM28, and clinicopathological features and survival prognosis in 145 cases of colorectal cancer and its adjacent tissues. HT29 cells of colorectal cancer were randomly divided into observation group and control group, respectively, transfection of sh TRIM28 down-expression plasmid and no-load control plasmid for 48 h. Western blot assay was used to detect TRIM28, in HT29 cells. The proliferation and migration of HT29 cells were observed by MTT assay and scratch assay. Results the expression of TRIM28 was higher in 97 cases of colorectal cancer than in 4 cases of adjacent tissues (P0.01). Moreover, the expression of TRIM28 in patients with lymph node metastasis, distant metastasis and high Dukes stage was higher than that without lymph node metastasis, distant metastasis and low Dukes stage (P 0.05). The 3-year disease-free survival rate of patients with high TRIM28 expression was 68.0%. 83.3% of the patients with low expression of TRIM28 were lower than those with low expression of TRIM28 (P0.01). Multivariate analysis of COX proportional risk model showed that TRIM28 expression was an independent prognostic factor (HR=3.26,P=0.01) in colorectal cancer patients. The relative expression of TRIM28 in HT29 cells in the observation group was lower than that in the control group (P0.01), and the OD value of proliferation and the migration distance of the cells in the observation group were lower than those in the control group (P0.01). Conclusion the high expression of TRIM28 in colorectal cancer can inhibit the proliferation and migration of colorectal cancer cells, and it may play an important role in the development of colorectal cancer, and it can be used as a prognostic index and a potential therapeutic target in colorectal cancer patients.
【作者单位】：河南中医药大学;
【基金】：河南省高等学校重点科研项目(16A310021)
【分类号】：R735.34

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