胃癌病理特征与HIF-1α和VEGF mRNA表达水平的关系

发布时间：2019-01-25 21:24

【摘要】：目的：探讨胃癌病理特征与HIF-la和VEGF mRNA表达水平的关系方法：采集67例胃癌病人术前的胃镜标本,采用半定量RT-PCR的方法检测胃癌组织及周围正常组织中的HIF-la和VEGF mRNA的表达水平。HIF-1α和VEGF mRNA的表达水平的评价采用癌组织(tumor)和正常组织(normal)的增值比例来表示。同时对所有胃癌患者临床病理特征进行回顾性分析。结果：HIF-1α mRNA的表达中,癌组织高于正常组织,并其T/N值与胃癌的淋巴转移、远处转移、分期呈正相关(P0.05),与胃癌分化程度呈无统计学相关性(p0.05)。VEGF mRNA的表达同样是癌组织高于正常组织,其T/N值与淋巴转移和远处转移呈正相关(P0.05),与胃癌分化程度呈无统计学相关性(p0.05)。结论：HIF-1α和VEGF mRNA表达水平越高的胃癌侵袭性越强。通过对胃癌的胃镜活检标本行RT-PCR检测HIF-1α和VEGF mRNA表达水平可以对胃癌的侵袭性进行术前评估。
[Abstract]:Objective: to investigate the relationship between pathological features of gastric cancer and the expression of HIF-la and VEGF mRNA. The expression of HIF-la and VEGF mRNA in gastric carcinoma and surrounding normal tissues was detected by semi-quantitative RT-PCR, and the expression of HIF-1 伪 and VEGF mRNA was evaluated by the ratio of (tumor) and (normal). The clinicopathological features of all patients with gastric cancer were analyzed retrospectively. Results: the expression of HIF-1 伪 mRNA in cancer tissue was higher than that in normal tissue, and the T / N value was positively correlated with lymph node metastasis, distant metastasis and staging of gastric cancer (P0.05). There was no significant correlation between the expression of p0. 05). VEGF mRNA and the degree of differentiation of gastric cancer (p0. 05). VEGF mRNA expression was higher than that of normal tissue, and the T / N value was positively correlated with lymphatic metastasis and distant metastasis (P0.05). There was no statistical correlation with the differentiation of gastric cancer (p0.05). Conclusion: the higher the expression of HIF-1 伪 and VEGF mRNA, the stronger the invasion of gastric cancer. The expression of HIF-1 伪 and VEGF mRNA in gastric cancer specimens were detected by RT-PCR, and the invasiveness of gastric cancer could be evaluated before operation.
【学位授予单位】：山东大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R735.2

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