应用基于色谱质谱联用技术的蛋白质组学鉴定筛选胃癌相关蛋白质

发布时间：2019-02-13 16:18

【摘要】：目的应用基于色谱质谱联用技术(LC-MS)的蛋白质组学鉴定筛选胃癌相关蛋白质,并初步探讨其功能。方法选取2011年4月—2012年9月于河北医科大学第四医院确诊为胃腺癌并进行手术切除者16例为研究对象。术中取患者胃癌组织及癌旁正常组织,采用基于LC-MS的蛋白质组学鉴定筛选胃癌组织及癌旁正常组织中的差异蛋白质,并应用免疫组化染色法验证筛选出的差异蛋白质。结果基于LC-MS的蛋白质组学鉴定结果显示,胃癌组织与癌旁正常组织均鉴定了3 000~4 000种蛋白质,其中12种蛋白质差异显著,将其与GO数据库进行对比,去除二级节点中归类为"细胞组分"的结构蛋白质,在"分子功能"和"代谢过程"这两个节点中找到了4种可能与胃癌有关的蛋白质,即谷胱甘肽巯基转移酶-π-1(GST-π-1)、铜锌超氧化物歧化酶(Cu/Zn-SOD)、丙酮酸激酶M2(PK-M2)、P120连环蛋白(P120-ctn)。免疫组化染色结果显示,胃癌组织中GST-π-1、PK-M2、P120-ctn阳性率大于癌旁正常组织,Cu/Zn-SOD阳性率小于癌旁正常组织(P0.001),与基于LC-MS的蛋白质组学鉴定结果相符。结论基于LC-MS的蛋白质组学鉴定可筛选出胃癌相关蛋白质(GST-π-1、Cu/Zn-SOD、PK-M2、P120-ctn),初步分析认为其与胃癌的多种细胞生物学活动有关,证实基于LC-MS的蛋白质组学鉴定在胃癌相关蛋白质筛选方面具有明显的应用价值。
[Abstract]:Objective to screen gastric cancer related proteins by proteomics based on LC-MS and to explore their functions. Methods from April 2011 to September 2012, 16 cases of gastric adenocarcinoma were diagnosed and surgically resected from the fourth Hospital of Hebei Medical University. The differentially expressed proteins in gastric cancer tissues and adjacent normal tissues were screened by proteomics identification based on LC-MS, and the differentially identified proteins were verified by immunohistochemical staining. Results the results of proteomics analysis based on LC-MS showed that 3 000 ~ 4 000 proteins were identified in gastric cancer tissues and adjacent normal tissues, among which 12 proteins had significant differences. The results were compared with GO database. Removing structural proteins classified as "cellular components" in secondary nodes, four proteins that might be associated with gastric cancer were found in the "molecular function" and "metabolic processes". They are glutathione mercaptotransferase-蟺 -1 (GST- 蟺 -1), copper zinc superoxide dismutase (Cu/Zn-SOD), pyruvate kinase M2 (PK-M2) and P120 catenin (P120-ctn). The results of immunohistochemical staining showed that the positive rate of GST- 蟺 -1 PK-M2P P120-ctn was higher than that of adjacent normal tissues, and the positive rate of Cu/Zn-SOD was lower than that of adjacent normal tissues (P0.001). The results were consistent with the results of proteomics identification based on LC-MS. Conclusion the proteome identification based on LC-MS can screen out GST- 蟺 -1CU / Zn-SODN-PK-M2P120-ctn, which is related to many cell biological activities of gastric cancer. It is confirmed that proteomics based on LC-MS is of great value in the screening of gastric cancer related proteins.
【作者单位】：河北医科大学第四医院外三科;河北医科大学第四医院肝胆外科;河北医科大学第四医院内镜室;
【基金】：国家自然科学基金资助项目(81372580) 河北省科技支撑项目(11276103D-26) 河北省医学科学研究重点课题计划项目(20160168)
【分类号】：R735.2

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