上皮间质转化相关标志物在结肠癌及结肠癌肝转移组织表达差异研究
发布时间：2019-02-13 20:23
【摘要】：目的:检测上皮间质转化(epithelial-mesenchymal transition,EMT)相关标志物E钙黏素、波形蛋白在无远处转移的结肠癌组织、伴有肝转移的结肠癌组织及结肠癌肝转移组织的表达情况,同时分析结肠癌组织中E钙黏素表达在不同临床病理特征之间表达差异性,进一步探讨EMT在结肠癌转移中的意义。方法:收集2011年1月至2014年12月山西医科大学第一医院及山西省肿瘤医院收治的未发生远处转移的结肠癌患者(15例)及结肠癌伴肝转移且同期行手术切除患者(20例)的结肠癌组织、结肠癌肝转移组织病理蜡块(石蜡包埋标本)。采用免疫组化方法(SP法)检测相应癌组织的E钙黏素、波形蛋白的表达情况。随机选择10个高倍视野(40×),每个视野计数100个细胞,以不表达或阳性细胞数25%判为(-),当细胞染色较强或阳性染细胞数75%判为(+),其余阳性细胞数25%~75%判为(±),其中(-)、(±)均视为阴性,并测量组织中蛋白表达平均灰度值。利用SPSS软件进行数据分析,定量资料之间比较采用t检验,计数资料之间比较采用χ2检验,均以双侧检验P0.05表示差异具有统计学意义。结果:(1)E钙黏素表达在无远处转移及有远处转移的结肠癌组织表达阳性率分别为86.7%(13/15)、35.7%(5/14),差异具有统计学意义(P0.05);(2)E钙黏素主要表达于细胞膜,在结肠癌组织及同其期肝转移组织中阳性表达为分别为25%(5/20)、40%(8/20),平均灰度值为173.075±29.770、175.540±26.5738,差异不具有统计学意义(P0.05);波形蛋白在结肠癌组织、结肠癌肝转移组织中间质细胞表达明显,并偶见肿瘤细胞胞浆表达,在两组内阳性表达率分别为80%(16/20)、65%(13/20),平均灰度值分别为110.079±22.434、100.187±13.891,差异不具有统计学意义(P0.05);(3)E钙黏素在结肠癌组织表达与性别、年龄、肿瘤大小、分化程度、浸润深度等临床病理特征之间表达差异不具有统计学意义(P0.05)。结论:(1)E钙黏素在未发生远处转移和已发生远处转移结肠癌组织中的表达差异对评估结肠癌转移风险可能具有重要的参考价值。(2)EMT标志物(E钙黏素、波形蛋白)在结肠癌组织及同期肝转移组织中表达水平相似,提示早期结肠癌细胞发生上皮间质转化,有利于肿瘤的发生发展,当肿瘤转移至新的部位,可能又发生间质上皮转化,有利于肿瘤在新的环境(如肝脏)中生长。(3)肿瘤组织的异质性造成不同区域组织生物学特性的不完全一致可能是分子标记物表达不均匀的原因。
[Abstract]:Objective: to detect the expression of E-cadherin and vimentin in colon cancer tissues without distant metastasis, colon cancer tissues with liver metastasis and colon cancer liver metastases. At the same time, we analyzed the difference of E-cadherin expression among different clinicopathological features in colon cancer tissues, and further discussed the significance of EMT in colon cancer metastasis. Methods: from January 2011 to December 2014, 15 patients with colon cancer without distant metastasis and 15 patients with colon cancer accompanied with liver metastasis who were admitted to the first Hospital of Shanxi Medical University and Shanxi Cancer Hospital were collected. (20 cases) of colon cancer, Pathological wax masses (paraffin embedded specimens) of liver metastases from colon cancer. The expression of E-cadherin and vimentin was detected by immunohistochemical method (SP). Ten high-power visual fields (40 脳) were randomly selected. 100 cells were counted in each field, and 25% of the cells without expression or positive were judged as (-), and 75% of the cells with strong staining or positive staining were judged as (),. 75% of the other positive cells were found to be (卤), of which (-), (卤) were considered negative, and the average gray value of protein expression was measured. The data were analyzed by SPSS software. T test was used to compare the quantitative data and 蠂 2 test was used to compare the count data. Results: (1) the positive rates of E-cadherin expression were 86.7% (13 / 15) and 35.7% (5 / 14) in colon cancer tissues with no distant metastasis and distant metastasis respectively (P0.05). (2) E-cadherin was mainly expressed on cell membrane. The positive expression of E-cadherin was 25% (5 / 20) and 40% (8 / 20) in colon cancer and liver metastasis respectively, and the average gray value was 173.075 卤29.770175.540 卤26.5738. The difference was not statistically significant (P0.05). The expression of vimentin in the stromal cells of colon cancer and liver metastases was obvious, and the expression of vimentin in the cytoplasm of tumor cells was occasionally observed. The positive expression rates of vimentin in the two groups were 80% (16 / 20) and 65% (13 / 20), respectively. The average gray value was 110.079 卤22.434100.187 卤13.891respectively, the difference was not statistically significant (P0.05). (3) there was no significant difference between the expression of E-cadherin and clinicopathological features such as sex, age, tumor size, differentiation, depth of invasion and so on (P0.05). Conclusion: (1) the difference of E-cadherin expression in colorectal cancer tissues without or without distant metastasis may be of important value in assessing the risk of colon cancer metastasis. (2) the EMT marker (E-cadherin) may be useful in evaluating the risk of colon cancer metastasis. The expression level of vimentin in colon cancer tissues and liver metastasis tissues is similar, suggesting that the epithelial interstitial transformation of colon cancer cells occurs in the early stage, which is beneficial to the development of tumor, and when the tumor metastases to a new site, the expression of vimentin is similar. Another transition of the interstitial epithelium may occur. (3) the heterogeneity of tumor tissues may cause the heterogeneity of tissue biological characteristics in different regions, which may be the reason for the uneven expression of molecular markers.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R735.35
[Abstract]:Objective: to detect the expression of E-cadherin and vimentin in colon cancer tissues without distant metastasis, colon cancer tissues with liver metastasis and colon cancer liver metastases. At the same time, we analyzed the difference of E-cadherin expression among different clinicopathological features in colon cancer tissues, and further discussed the significance of EMT in colon cancer metastasis. Methods: from January 2011 to December 2014, 15 patients with colon cancer without distant metastasis and 15 patients with colon cancer accompanied with liver metastasis who were admitted to the first Hospital of Shanxi Medical University and Shanxi Cancer Hospital were collected. (20 cases) of colon cancer, Pathological wax masses (paraffin embedded specimens) of liver metastases from colon cancer. The expression of E-cadherin and vimentin was detected by immunohistochemical method (SP). Ten high-power visual fields (40 脳) were randomly selected. 100 cells were counted in each field, and 25% of the cells without expression or positive were judged as (-), and 75% of the cells with strong staining or positive staining were judged as (),. 75% of the other positive cells were found to be (卤), of which (-), (卤) were considered negative, and the average gray value of protein expression was measured. The data were analyzed by SPSS software. T test was used to compare the quantitative data and 蠂 2 test was used to compare the count data. Results: (1) the positive rates of E-cadherin expression were 86.7% (13 / 15) and 35.7% (5 / 14) in colon cancer tissues with no distant metastasis and distant metastasis respectively (P0.05). (2) E-cadherin was mainly expressed on cell membrane. The positive expression of E-cadherin was 25% (5 / 20) and 40% (8 / 20) in colon cancer and liver metastasis respectively, and the average gray value was 173.075 卤29.770175.540 卤26.5738. The difference was not statistically significant (P0.05). The expression of vimentin in the stromal cells of colon cancer and liver metastases was obvious, and the expression of vimentin in the cytoplasm of tumor cells was occasionally observed. The positive expression rates of vimentin in the two groups were 80% (16 / 20) and 65% (13 / 20), respectively. The average gray value was 110.079 卤22.434100.187 卤13.891respectively, the difference was not statistically significant (P0.05). (3) there was no significant difference between the expression of E-cadherin and clinicopathological features such as sex, age, tumor size, differentiation, depth of invasion and so on (P0.05). Conclusion: (1) the difference of E-cadherin expression in colorectal cancer tissues without or without distant metastasis may be of important value in assessing the risk of colon cancer metastasis. (2) the EMT marker (E-cadherin) may be useful in evaluating the risk of colon cancer metastasis. The expression level of vimentin in colon cancer tissues and liver metastasis tissues is similar, suggesting that the epithelial interstitial transformation of colon cancer cells occurs in the early stage, which is beneficial to the development of tumor, and when the tumor metastases to a new site, the expression of vimentin is similar. Another transition of the interstitial epithelium may occur. (3) the heterogeneity of tumor tissues may cause the heterogeneity of tissue biological characteristics in different regions, which may be the reason for the uneven expression of molecular markers.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R735.35
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