SIRT1对肺癌A549细胞增殖和迁移的影响

发布时间：2019-07-30 14:46

【摘要】：目的通过调节肺癌A549细胞中沉默信息调节因子1(SIRT1)的表达,探讨SIRT1对肺癌细胞增殖和转移的影响及其分子机制。方法培养肺癌A549细胞,用重组腺病毒感染A549细胞上调/下调SIRT1的表达;通过MTT实验、克隆形成实验、划痕实验和Transwell迁移实验检测上调/下调SIRT1表达对肺癌A549细胞增殖和转移的影响;干扰SIRT1表达后用Western blot检测SIRT1对肺癌A549细胞中上皮细胞向间质细胞转化(EMT)相关蛋白标记物ZO-1、β-连环蛋白(β-catenin)、Snail和ZEB1表达的影响。结果上调/下调SIRT1表达对肺癌A549细胞增殖无明显影响,但是可以促进/抑制肺癌A549细胞迁移;下调SIRT1表达后EMT相关上皮性标记蛋白ZO-1表达增多,间质性标记蛋白β-catenin、Snail和ZEB1表达减少。结论 SIRT1可能通过影响EMT相关标记蛋白的表达促进肺癌A549细胞迁移。
[Abstract]:Objective to investigate the effect of SIRT1 on proliferation and metastasis of lung cancer cells and its molecular mechanism by regulating the expression of silencing information regulator 1 (SIRT1) in lung cancer cell line A549. Methods Lung cancer A549 cells were cultured and infected with recombinant adenoviruses to up-regulate / down-regulate the expression of SIRT1, and the effects of up-regulation / down-regulation of SIRT1 expression on the proliferation and metastasis of lung cancer A549 cells were detected by MTT assay, clone formation test, scratch test and Transwell migration assay. After interfering with SIRT1 expression, the effect of SIRT1 on the expression of (EMT) related protein markers ZO-1, 尾-catenin), Snail and ZEB1 from epithelial cells to stroma cells in lung cancer A549 cells was detected by Western blot. Results the up-regulation / down-regulation of SIRT1 expression had no significant effect on the proliferation of lung cancer cell line A549, but it could promote / inhibit the migration of lung cancer cell line A549. After down-regulating the expression of SIRT1, the expression of EMT-related epithelial marker protein ZO-1 increased and the expression of interstitial marker protein 尾-catenin,Snail and ZEB1 decreased. Conclusion SIRT1 may promote the migration of lung cancer cell line A549 by affecting the expression of EMT-related marker protein.
【作者单位】：广东医科大学呼吸疾病研究所;广东医科大学生物化学与分子生物学教研室;
【基金】：国家自然科学基金青年基金项目(编号:81400023) 广东医科大学优秀硕士论文培育项目(编号:4CX14091G)
【分类号】：R734.2

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