G蛋白偶联受体激酶2调控M2巨噬细胞β2肾上腺素受体信号在肝细胞癌中的作用
发布时间：2020-12-19 06:20
原发性肝细胞癌（hepatocellular carcinoma,HCC）约占原发性肝癌的90%,是一种高度恶性肿瘤,全球肿瘤相关死因第二位。由于HCC的病因尚未完全明确,并且缺乏特效的药物与治疗手段,HCC患者复发率高,预后较差。因此,关于HCC的新治疗方法迫切需要。肿瘤微环境是指肿瘤发生发展过程中所处的局部生物环境,主要包括免疫细胞、新生血管细胞、肿瘤相关成纤维细胞以及一些细胞外基质等。巨噬细胞为单核细胞谱系的单核吞噬白细胞,根据功能不同分为两型,即M1和M2型巨噬细胞。肿瘤微环境中浸润的巨噬细胞称为肿瘤相关巨噬细胞（tumor associated macrophages,TAM）,以M2型为主。研究表明,TAM与肿瘤进展相关。肝脏内分布大量的自主神经,包括交感神经和副交感神经。交感神经系统激活并释放去甲肾上腺素和肾上腺素,能够激活肿瘤细胞或宿主基质细胞的β肾上腺素能受体（β-adrenergic receptor,β-AR）,促进多种实体肿瘤的生长和转移。β2-AR是β-AR的亚型,属于G蛋白偶联受体（G protein-coupled receptor,GPCR）,具有七个跨...
【文章来源】：安徽医科大学安徽省
【文章页数】：113 页
【学位级别】：博士
【部分图文】：
不同分期肝癌患者石蜡切片CD163和CD206的共表达Fig1.Theco-expressionofCD163andCD206inparafinsectionofHCCpatientswithdifferentstages(controlgroupn=10,Ⅰ+Ⅱgroupn=34,Ⅲ+Ⅳgroupn=46)
图 2. CD163 和 CD206 在人 HCC 新鲜组织中的表达Fig 2. The expression of CD163 and CD206 in HCC tissues with different TNM stageA: The expression of CD163 was elevated in HCC tissues with Ⅳstage. The histogramrepresents the relative expression of CD163 in HCC tissues of different groups. B: The expressionof CD206 was elevated in HCC tissues with Ⅳ stage. The histogram represents the relativeexpression of CD206 in HCC tissues of different groups ( x±s, n=3).*P<0.05,**P<0.01 comparedwith control group;##P<0.01 compared with Ⅰ+Ⅱ stage group.4.3 肝癌患者肝组织切片 GRK2 和 CD163 的共表达减少，β2-AR 和 CD163 的共表达增多免疫荧光双染结果显示，在对照组中，只有很少的 CD163+细胞表达 β2-AR，而在肝癌患者肝组织切片中存在大量共表达 β2-AR 和 CD163 的细胞。GRK2 在
安徽医科大学博士学位论文半定量并分析 β2-AR 与 CD163、GRK2 与 CD163 的共表达在Ⅲ+Ⅳ期肝石蜡切片、Ⅰ+Ⅱ期肝癌石蜡切片及对照组织的表达发现，Ⅰ+Ⅱ期肝癌患者组织切片中 GRK2 和 CD163 的共表达较对照组减少，而 β2-AR 和 CD163 的表达增多；Ⅲ+Ⅳ期肝癌患者肝组织切片中 GRK2 和 CD163 的共表达较Ⅰ+ⅡHCC 组进一步减少，β2-AR 和 CD163 的共表达进一步增多（图 4）。
【参考文献】：
期刊论文
[1]Role of intrahepatic innervation in regulating the activity of liver cells[J]. Letitia Adela Maria Streba,Cristin Constantin Vere,Alin Gabriel Ionescu,Costin Teodor Streba,Ion Rogoveanu. World Journal of Hepatology. 2014(03)
[2]Hepatocellular carcinoma and macrophage interaction induced tumor immunosuppressionvia Treg requires TLR4 signaling[J]. Jing Yang, Jin-Xiang Zhang, Hui Wang, Guo-Liang Wang, Qing-Gang Hu, Qi-Chang Zheng, Department of Hepatobiliary Surgery, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China , Department of Emergency Surgery, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China , Department of Medical Genetics, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China. World Journal of Gastroenterology. 2012(23)
[3]Significance and relationship between infiltrating inflammatory cell and tumor angiogenesis in hepatocellular carcinoma tissues[J]. Shao-Hua Peng, Hong Deng, Jian-Feng Yang, Ping-Ping Xie, Cheng Li, Hao Li, Department of Pathology, Medical College, Hunan Normal University, Changsha 410006, Hunan Province, China De-Yun Feng, Department of Pathology, Xiangya School of Medicine, Central South University, Changsha 410078, Hunan Province, China. World Journal of Gastroenterology. 2005(41)
本文编号：2925418
【文章来源】：安徽医科大学安徽省
【文章页数】：113 页
【学位级别】：博士
【部分图文】：
不同分期肝癌患者石蜡切片CD163和CD206的共表达Fig1.Theco-expressionofCD163andCD206inparafinsectionofHCCpatientswithdifferentstages(controlgroupn=10,Ⅰ+Ⅱgroupn=34,Ⅲ+Ⅳgroupn=46)
图 2. CD163 和 CD206 在人 HCC 新鲜组织中的表达Fig 2. The expression of CD163 and CD206 in HCC tissues with different TNM stageA: The expression of CD163 was elevated in HCC tissues with Ⅳstage. The histogramrepresents the relative expression of CD163 in HCC tissues of different groups. B: The expressionof CD206 was elevated in HCC tissues with Ⅳ stage. The histogram represents the relativeexpression of CD206 in HCC tissues of different groups ( x±s, n=3).*P<0.05,**P<0.01 comparedwith control group;##P<0.01 compared with Ⅰ+Ⅱ stage group.4.3 肝癌患者肝组织切片 GRK2 和 CD163 的共表达减少，β2-AR 和 CD163 的共表达增多免疫荧光双染结果显示，在对照组中，只有很少的 CD163+细胞表达 β2-AR，而在肝癌患者肝组织切片中存在大量共表达 β2-AR 和 CD163 的细胞。GRK2 在
安徽医科大学博士学位论文半定量并分析 β2-AR 与 CD163、GRK2 与 CD163 的共表达在Ⅲ+Ⅳ期肝石蜡切片、Ⅰ+Ⅱ期肝癌石蜡切片及对照组织的表达发现，Ⅰ+Ⅱ期肝癌患者组织切片中 GRK2 和 CD163 的共表达较对照组减少，而 β2-AR 和 CD163 的表达增多；Ⅲ+Ⅳ期肝癌患者肝组织切片中 GRK2 和 CD163 的共表达较Ⅰ+ⅡHCC 组进一步减少，β2-AR 和 CD163 的共表达进一步增多（图 4）。
【参考文献】：
期刊论文
[1]Role of intrahepatic innervation in regulating the activity of liver cells[J]. Letitia Adela Maria Streba,Cristin Constantin Vere,Alin Gabriel Ionescu,Costin Teodor Streba,Ion Rogoveanu. World Journal of Hepatology. 2014(03)
[2]Hepatocellular carcinoma and macrophage interaction induced tumor immunosuppressionvia Treg requires TLR4 signaling[J]. Jing Yang, Jin-Xiang Zhang, Hui Wang, Guo-Liang Wang, Qing-Gang Hu, Qi-Chang Zheng, Department of Hepatobiliary Surgery, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China , Department of Emergency Surgery, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China , Department of Medical Genetics, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China. World Journal of Gastroenterology. 2012(23)
[3]Significance and relationship between infiltrating inflammatory cell and tumor angiogenesis in hepatocellular carcinoma tissues[J]. Shao-Hua Peng, Hong Deng, Jian-Feng Yang, Ping-Ping Xie, Cheng Li, Hao Li, Department of Pathology, Medical College, Hunan Normal University, Changsha 410006, Hunan Province, China De-Yun Feng, Department of Pathology, Xiangya School of Medicine, Central South University, Changsha 410078, Hunan Province, China. World Journal of Gastroenterology. 2005(41)
本文编号：2925418
本文链接：https://www.wllwen.com/yixuelunwen/zlx/2925418.html
