胃腺癌血清非炎症性标志物的筛
发布时间：2021-03-06 16:56
研究背景胃腺癌是胃癌的一种,源于胃腺体细胞恶化,占全部胃癌发病率的95%。早期诊断可明显改善癌症治疗效果,但现实却是由于缺乏早期诊断手段,往往就诊时多数患者已经处于局部晚期或发生了远处转移从而延误了病情,错失最佳的治疗时机。血清肿瘤标志物以其简便、快捷、无痛苦等优点,成为胃癌患者的理想检查指标,目前临床常见的胃癌辅助诊断中有癌胚抗原（CEA）、糖类抗原125（CA125）及糖类抗原199（CA19-9）。尽管多种肿瘤标志物联合检测能够在一定程度上提高早期胃癌诊断的敏感度,可用于预测患者的病情进展和预后,但检测重复性不佳。组合应用表面增强激光解析电离飞行时间质谱（SELDI-TOF-MS）、高效液相色谱（HPLC）及基质辅助激光解析附电离飞行时间质谱（MALDI-TOF-MS）技术,课题组前期曾成功在乳头状甲状腺癌及乳腺癌中寻找特异性标志物,对相关肿瘤早期诊断及预后检测有参考意义,但前期实验存在以下问题:a)血清特异性标志物未与临床在使用的肿瘤标志物做对比;b)特异性标志物未排除炎症干扰;c)血清分析测试前各种影响因素存在偏差,且相互不独立,影响检验效能。尽管近年来分子生物标志物的发现急...
【文章来源】：郑州大学河南省 211工程院校
【文章页数】：133 页
【学位级别】：博士
【部分图文】：
图１探索组及测试组患者的生存资料及探索?
图２探索组不同血清及胃癌不同分期中ｍ／Ｚ?６４４９?Ｄａ蛋白质谱图、模拟电泳图及表达强度??图。注：Ｃｏｎ－ｈ：正常对照，Ｃｏｎ－ｇ：胃炎对照，ＧＣ－ｐｒｅ：胃癌术前，ＧＣ－ｐｏｓ：胃癌术后，??ＧＣ－ｒｅｌ：胃癌复发；ＧＣ－丨：丨期胃癌，ＧＣ－丨丨：１丨期胃癌，ＧＣ－丨丨丨：丨丨１期胃癌，ＧＣ－ＩＶ：?ＩＶ期??胃癌。＊ｐ＜０．０５。??３．?２判别模型盲法验证??为验证上述得到的判别模型的准确性和可行性，本研宄用测试组［６０例胃癌??１９??
?第一部分：胃腺癌非炎症性血清标志物的筛选、鉴定及验证???同时检测６４４９?Ｄａ在胃癌血清及组织中的表达情况，结果发现：血清ＷＢ：与对??照组相比，ｍ／ｚ６４４９Ｄａ蛋白在术前组高表达，术后纟且低表达，复发组又表达上??调（对照，０．３０６±０．１３８?ｖｓ?术前，１．０７５±０．１２３?ｖｓ?术后，０．５１２士０．１４８?ｖｓ?复发，??０．７７８±０．１０６；ｐ＜０．０５；图?３Ｆ，?Ｇ）；血清?ＥＬＩＳＡ?结果与血清?ＷＢ?结果相一致，ｍ／ｚ??６４４９?Ｄａ蛋白在术前组高表达，术后组低表达，复发组又表达上调（对照，??０．５１６±〇．１８２ｖｓ?术前，２．０４３±０．９４０?ｖｓ?术后，１．１６５±０．４４２?ｖｓ?复发，１．５１８±０．３０９；??ｐ＜０．０５；图３Ｈ）；组织ＷＢ：?ｍ／ｚ?６４４９?Ｄａ蛋白表达在胃癌上皮与对照上皮组织??之间表达无统计学差异（对照，０．１７５?±?０．０２７?ｖｓ胃癌，０．１８２?±?０．０７８；?ｔ＝??０．０８９，ｐ＝０．９３４；图?４Ａ，Ｂ）；组织?ＥＬＩＳＡ?结果与组织?ＷＢ?结果相一致，ｍ／ｚ６４４９Ｄａ??蛋白表达在胃癌上皮与对照上皮组织之间表达无统计学差异（对照，０．２６７±０．０３１??ｖｓ?胃癌，０．３０８?±０．１９６；?ｔ＝ｌ．７８９，ｐ＝０．０７６；图?４Ｃ）。??￣Ｋ?［Ｂ?ｒｃ－??－－
【参考文献】：
期刊论文
[1]Prognostic value of systemic immune-inflammation index in patients with gastric cancer[J]. Kang Wang,Feiyu Diao,Zhijun Ye,Xinhua Zhang,Ertao Zhai,Hui Ren,Tong Li,Hui Wu,Yulong He,Shirong Cai,Jianhui Chen. Chinese Journal of Cancer. 2017(09)
[2]Clinical significance of expression of proliferating cell nuclear antigen and E-cadherin in gastric carcinoma[J]. lin Hu,Hong-lang li,Wei-feng li,Jun-min Chen,Jian-tao Yang,Jun-Jing Gu,lin Xin. World Journal of Gastroenterology. 2017(20)
[3]Hes1,an important gene for activation of hepatic stellate cells,is regulated by Notch1 and TGF-β/BMP signaling[J]. Kai Zhang,Yan-Qiong Zhang,Wen-Bing Ai,Qing-Ting Hu,Qiao-Juan Zhang,Lin-Yan Wan,Xiao-Lian Wang,Chang-Bai Liu,Jiang-Feng Wu. World Journal of Gastroenterology. 2015(03)
[4]Xiaotan Sanjie decoction attenuates tumor angiogenesis by manipulating Notch-1-regulated proliferation of gastric cancer stem-like cells[J]. Bing Yan,Long Liu,Ying Zhao,Li-Juan Xiu,Da-Zhi Sun,Xuan Liu,Ye Lu,Jun Shi,Yin-Cheng Zhang,Yong-Jin Li,Xiao-Wei Wang,Yu-Qi Zhou,Shou-Han Feng,Can Lv,Pin-Kang Wei,Zhi-Feng Qin. World Journal of Gastroenterology. 2014(36)
[5]Surgical resection of advanced gastric cancer following trastuzumab/oxaliplatin/capecitabine combination therapy[J]. Cai-Xia Dong,Jian-Fei Fu,Xian-Yun Ye,Xiao-Fen Li,Xian Zhong,Ying Yuan. World Journal of Gastroenterology. 2014(34)
[6]DNA and histone methylation in gastric carcinogenesis[J]. Danielle Queiroz Calcagno,Carolina Oliveira Gigek,Elizabeth Suchi Chen,Rommel Rodriguez Burbano,Marília de Arruda Cardoso Smith. World Journal of Gastroenterology. 2013(08)
[7]中国恶性肿瘤发病趋势分析和预测[J]. 陈万青,郑荣寿,曾红梅,张思维,李霓,邹小农,赫捷. 中华预防医学杂志. 2012 (07)
[8]Notch1和PTEN在胃癌组织中的表达及其意义[J]. 李大卫,吴晴,彭志海,杨兆瑞,王一. 癌症. 2007(11)
[9]Protein profile of human hepatocarcinoma cell line SMMC-7721:Identification and functional analysis[J]. Yi Feng, Zhong-Min Tian, Ming-Xi Wan, Zhao-Bin Zheng The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Department of Biomedical Engineering, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710049, Shaanxi Province, China. World Journal of Gastroenterology. 2007(18)
[10]Identification of serum proteins discriminating colorectal cancer patients and healthy controls using surface-enhanced laser desorption ionisation-time of flight mass spectrometry[J]. Judith YMN Engwegen,Helgi H Helgason,Annemieke Cats,Nathan Harris,Johannes MG Bonfrer,Jan HM Schellens,Jos H Beijnen. World Journal of Gastroenterology. 2006(10)
本文编号：3067464
【文章来源】：郑州大学河南省 211工程院校
【文章页数】：133 页
【学位级别】：博士
【部分图文】：
图１探索组及测试组患者的生存资料及探索?
图２探索组不同血清及胃癌不同分期中ｍ／Ｚ?６４４９?Ｄａ蛋白质谱图、模拟电泳图及表达强度??图。注：Ｃｏｎ－ｈ：正常对照，Ｃｏｎ－ｇ：胃炎对照，ＧＣ－ｐｒｅ：胃癌术前，ＧＣ－ｐｏｓ：胃癌术后，??ＧＣ－ｒｅｌ：胃癌复发；ＧＣ－丨：丨期胃癌，ＧＣ－丨丨：１丨期胃癌，ＧＣ－丨丨丨：丨丨１期胃癌，ＧＣ－ＩＶ：?ＩＶ期??胃癌。＊ｐ＜０．０５。??３．?２判别模型盲法验证??为验证上述得到的判别模型的准确性和可行性，本研宄用测试组［６０例胃癌??１９??
?第一部分：胃腺癌非炎症性血清标志物的筛选、鉴定及验证???同时检测６４４９?Ｄａ在胃癌血清及组织中的表达情况，结果发现：血清ＷＢ：与对??照组相比，ｍ／ｚ６４４９Ｄａ蛋白在术前组高表达，术后纟且低表达，复发组又表达上??调（对照，０．３０６±０．１３８?ｖｓ?术前，１．０７５±０．１２３?ｖｓ?术后，０．５１２士０．１４８?ｖｓ?复发，??０．７７８±０．１０６；ｐ＜０．０５；图?３Ｆ，?Ｇ）；血清?ＥＬＩＳＡ?结果与血清?ＷＢ?结果相一致，ｍ／ｚ??６４４９?Ｄａ蛋白在术前组高表达，术后组低表达，复发组又表达上调（对照，??０．５１６±〇．１８２ｖｓ?术前，２．０４３±０．９４０?ｖｓ?术后，１．１６５±０．４４２?ｖｓ?复发，１．５１８±０．３０９；??ｐ＜０．０５；图３Ｈ）；组织ＷＢ：?ｍ／ｚ?６４４９?Ｄａ蛋白表达在胃癌上皮与对照上皮组织??之间表达无统计学差异（对照，０．１７５?±?０．０２７?ｖｓ胃癌，０．１８２?±?０．０７８；?ｔ＝??０．０８９，ｐ＝０．９３４；图?４Ａ，Ｂ）；组织?ＥＬＩＳＡ?结果与组织?ＷＢ?结果相一致，ｍ／ｚ６４４９Ｄａ??蛋白表达在胃癌上皮与对照上皮组织之间表达无统计学差异（对照，０．２６７±０．０３１??ｖｓ?胃癌，０．３０８?±０．１９６；?ｔ＝ｌ．７８９，ｐ＝０．０７６；图?４Ｃ）。??￣Ｋ?［Ｂ?ｒｃ－??－－
【参考文献】：
期刊论文
[1]Prognostic value of systemic immune-inflammation index in patients with gastric cancer[J]. Kang Wang,Feiyu Diao,Zhijun Ye,Xinhua Zhang,Ertao Zhai,Hui Ren,Tong Li,Hui Wu,Yulong He,Shirong Cai,Jianhui Chen. Chinese Journal of Cancer. 2017(09)
[2]Clinical significance of expression of proliferating cell nuclear antigen and E-cadherin in gastric carcinoma[J]. lin Hu,Hong-lang li,Wei-feng li,Jun-min Chen,Jian-tao Yang,Jun-Jing Gu,lin Xin. World Journal of Gastroenterology. 2017(20)
[3]Hes1,an important gene for activation of hepatic stellate cells,is regulated by Notch1 and TGF-β/BMP signaling[J]. Kai Zhang,Yan-Qiong Zhang,Wen-Bing Ai,Qing-Ting Hu,Qiao-Juan Zhang,Lin-Yan Wan,Xiao-Lian Wang,Chang-Bai Liu,Jiang-Feng Wu. World Journal of Gastroenterology. 2015(03)
[4]Xiaotan Sanjie decoction attenuates tumor angiogenesis by manipulating Notch-1-regulated proliferation of gastric cancer stem-like cells[J]. Bing Yan,Long Liu,Ying Zhao,Li-Juan Xiu,Da-Zhi Sun,Xuan Liu,Ye Lu,Jun Shi,Yin-Cheng Zhang,Yong-Jin Li,Xiao-Wei Wang,Yu-Qi Zhou,Shou-Han Feng,Can Lv,Pin-Kang Wei,Zhi-Feng Qin. World Journal of Gastroenterology. 2014(36)
[5]Surgical resection of advanced gastric cancer following trastuzumab/oxaliplatin/capecitabine combination therapy[J]. Cai-Xia Dong,Jian-Fei Fu,Xian-Yun Ye,Xiao-Fen Li,Xian Zhong,Ying Yuan. World Journal of Gastroenterology. 2014(34)
[6]DNA and histone methylation in gastric carcinogenesis[J]. Danielle Queiroz Calcagno,Carolina Oliveira Gigek,Elizabeth Suchi Chen,Rommel Rodriguez Burbano,Marília de Arruda Cardoso Smith. World Journal of Gastroenterology. 2013(08)
[7]中国恶性肿瘤发病趋势分析和预测[J]. 陈万青,郑荣寿,曾红梅,张思维,李霓,邹小农,赫捷. 中华预防医学杂志. 2012 (07)
[8]Notch1和PTEN在胃癌组织中的表达及其意义[J]. 李大卫,吴晴,彭志海,杨兆瑞,王一. 癌症. 2007(11)
[9]Protein profile of human hepatocarcinoma cell line SMMC-7721:Identification and functional analysis[J]. Yi Feng, Zhong-Min Tian, Ming-Xi Wan, Zhao-Bin Zheng The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Department of Biomedical Engineering, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710049, Shaanxi Province, China. World Journal of Gastroenterology. 2007(18)
[10]Identification of serum proteins discriminating colorectal cancer patients and healthy controls using surface-enhanced laser desorption ionisation-time of flight mass spectrometry[J]. Judith YMN Engwegen,Helgi H Helgason,Annemieke Cats,Nathan Harris,Johannes MG Bonfrer,Jan HM Schellens,Jos H Beijnen. World Journal of Gastroenterology. 2006(10)
本文编号：3067464
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