趋化因子CXCL7在结直肠癌临床诊断、增殖、迁移及侵袭的作用研究

发布时间：2021-06-25 09:23

　　目的:探究趋化因子CXCL7作为结直肠癌（colorectal cancer,CRC）生物标志物的诊断价值;比较CRC患者癌组织和癌旁组织的表达差异,并研究趋化因子CXCL7对CRC细胞生物学功能的影响。方法:ELISA法定量测量280名CRC患者和280名正常人群血清CXCL7的浓度,比较两组人群血清浓度的差异性,分析CRC患者血清CXCL7浓度与临床资料的相关性;通过测试队列、验证队列和交叉队列分析CXCL7的诊断效能;建立Logistic多元回归模型进行联合诊断,计算联合诊断因子,绘制ROC工作曲线,并计算AUC曲线下面积、灵敏度（Sensitivity）、特异度（Specificity）等指标;TNM、I-II、性别亚组分析进一步评价CXCL7的诊断效能;重新从不同医院选择CRC患者进行诊断试验,进一步检验CXCL7作为生物标记物的稳定性和外延推广价值。使用免疫组化法检测20名CRC患者癌组织和癌旁组织中CXCL7的表达情况,同时借助qPCR法测定患者癌组织和癌旁组织中CXCL7的表达水平,分析CXCL7在癌组织和癌旁组织的表达差异性。慢病毒转染构建LoVo过表达和Caco-2... 

【文章来源】：江南大学江苏省 211工程院校 教育部直属院校

【文章页数】：52 页

【学位级别】：硕士

【部分图文】：

CRC流行病学“三间分布”数据

第二章血清趋化因子CXCL7在CRC临床诊断中的价值探究13图2-1CRC患者和对照组血清CXCL7和肿瘤相关抗原浓度散点图Figure2-1.ScatterplotanalysisofserumconcentrationsofCXCL7andtumor-associatedantigensinpatientswithCRCandcontrols.(A)ComparisonofserumCXCL7concentrationsbetweenthecontrolandCRCgroups.(B)ComparisonofserumCXCL7concentrationsamongdifferentTNMsubgroups.(C–H)ComparisonsofserumCEA,CA125,andCA19-9levelsbetweenthecontrolandCRCgroupsandcomparisonsamongdifferentTNMsubgroups.TheMann-WhitneyU-testwasusedforcomparisonsbetweentwogroupsandtheKruskal-Wallistestwasappliedforanalysisofthreeormoregroups.*P<0.05,**P<0.01,***P<0.001,NS:Nostatisticalsignificance.表2-2CRC患者血清CXCL7与CRC患者临床病理参数的相关性分析Table2-2.CorrelationbetweenCXCL7levelandclinicalcharacteristicsinpatientswithCRCVariableNumberMedianIQRrPvalueAge(years)<601031.781.46-2.160.0750.211≥601771.891.51-2.27SexMale1661.921.52-2.29-0.1430.016Female1141.761.46-2.15Tumersize<4cm1711.821.50-2.28-0.0370.542≥4cm1091.821.48-2.19HistologicalgradeWell141.691.48-2.13-0.0120.836Moderately1991.841.53-2.22

第二章血清趋化因子CXCL7在CRC临床诊断中的价值探究1511514表2-4测试队列与验证队列诊断试验结果Table2-4.DiagnosticefficiencyofCXCL7determinedbyROCcurveanalysisintwosetsVariableMedianIQRCutoffAUC(95%CI)Sensitivity(%)Specificity(%)PvalueAsetAp1.841.51-2.211.430.872(0.826-0.910)81.9584.96<0.001Ac1.020.78-1.28BsetBp1.821.48-2.261.280.853(0.843-0.925)86.3980.95<0.001Bc1.010.82-1.23Cross-validationAp/Bc1.300.869(0.823-0.906)85.7181.63<0.001Bp/Ac1.300.859(0.812-0.897)84.3979.70<0.001图2-2测试队列与验证队列诊断试验结果Figure2-2.CXCL7diagnostictestintrainingandvalidationsets.(A)AUCinthetrainingset.(B)SensitivityandspecificityinrelationtoserumCXCL7concentrationinthetrainingset.(C)Youdenindexinthetrainingset.(D)AUCinthevalidationset.(E)Sensitivityandspecificityinthevalidationset.(F)Youdenindexinthevalidationset.(G)AUCintheAp/Bcset.(H)SensitivityandspecificityinrelationtoserumCXCL7concentrationintheAp/Bcset.(I)YoudenindexintheAp/Bcset.(J)AUCintheBp/Acset.(K)SensitivityandspecificityinrelationtoserumCXCL7concentrationintheBp/Acset.(L)YoudenindexintheBp/Acset.2.5.4不同TNM分期CRC患者血清CXCL7的诊断结果

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