龟板联合阿伦磷酸钠对激素性骨质疏松大鼠腰椎Runx2、CTSK表达的影响

发布时间：2017-12-31 15:17

  本文关键词：龟板联合阿伦磷酸钠对激素性骨质疏松大鼠腰椎Runx2、CTSK表达的影响　出处：《中华中医药杂志》2017年05期 　论文类型：期刊论文

  更多相关文章： 激素性骨质疏松 龟板 阿伦磷酸钠 协同作用 CTSK Runx

【摘要】：目的:观察龟板联合阿伦磷酸钠(ALN)对激素性骨质疏松(GIOP)大鼠腰椎Runx2、CTSK表达的影响。方法:40只3月龄雌性SD大鼠随机分为4组:空白组、模型组、ALN组、龟板联合ALN组(联合组)。模型组、ALN组及联合组皮下注射地塞米松造模,成功后分别用0.9%氯化钠溶液、ALN和龟板联合ALN进行灌胃。12周后取大鼠腰椎进行:micro-CT检测腰椎骨微细结构、HE染色观察骨组织形态学,qPCR及Western blot分别检测腰椎Runx2、CTSK mRNA和蛋白表达。结果:与空白组比较,模型组Tb.N、Tb.Th明显下降(P0.01),Tb.Sp、CTSK mRNA和蛋白表达明显升高(P0.01),Runx2 mRNA和蛋白表达呈下调趋势;与模型组比较,ALN组和联合组BS/TV、Tb.N、Tb.Th、Runx2蛋白表达明显升高(P0.01),Tb.Sp、CTSK mRNA和蛋白表达明显下调(P0.01)。结论:龟板联合阿伦磷酸钠抗大鼠腰椎GIOP的协同机制可能与调节Runx2、CTSK的表达密切相关。
[Abstract]:Objective: To observe the effect of Plastrum Testudinis combined with Allen sodium phosphate (ALN) on glucocorticoid induced osteoporosis (GIOP) rats lumbar Runx2, CTSK expression. Methods: 40 March old female SD rats were randomly divided into 4 groups: control group, model group, ALN group, combined group ALN (combined group). The model of turtle shell group, ALN group and combined group subcutaneous injection of dexamethasone model, after the success of Sodium Chloride Solution ALN and 0.9% respectively, combined with ALN.12 by gavage Testudinis weeks after rat lumbar micro-CT detection of lumbar bone micro structure of bone tissue morphology, HE staining, qPCR and Western blot respectively to detect expression of CTSK mRNA Runx2 of the lumbar spine. And protein. Results: compared with the control group, Tb.N model group, Tb.Th decreased significantly (P0.01), Tb.Sp, mRNA and expression of CTSK protein increased significantly (P0.01), the expression of Runx2 mRNA and protein decreased; compared with the model group, ALN group and combined group BS/TV, Tb.N, Tb.Th, Runx2 protein expression in Ming Dynasty Xian Shenggao (P0.01), Tb.Sp, mRNA and protein expression of CTSK was significantly reduced (P0.01). Conclusion: the coordination mechanism of sodium phosphate in rats with Allen turtle lumbar GIOP may be related to the regulation of Runx2, the expression of CTSK is closely related.

【作者单位】： 广州中医药大学;广州中医药大学第一附属医院;
【基金】：国家自然科学基金项目(No.81503591,No.81674000) 广东省科技厅项目(No.2014 A020221021,No.2016A020226006) 广东省自然科学基金项目(No.2014A030310082,No.2016A030313645) 广州中医药大学及第一临床医学院优秀博士论文培育项目(No.Y B201501,No.Y B201602);广州中医药大学优秀青年学者科研基金项目(No.KAB110133K04) 广州中医药大学第一附属医院创新强院项目(No.2015Q N03) 广东省教育厅学科建设专项基金(育苗工程)(No.2013LYM-0012)~~
【分类号】：R245
【正文快照】： 糖皮质激素性骨质疏松症(glucocor ticoid-inducedosteoporosis,GIOP)是最常见继发性骨质疏松症,是药用糖皮质激素(glucocorticoid,GC)常见不良反应之一,其防治和机制研究引起国内外学者普遍重视[1]。二磷酸盐可抑制骨重建、提高骨强度、降低骨折率,是GIOP一线用药[2],但其具，

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