电针对鱼藤酮诱导的帕金森病模型大鼠黑质内自噬相关蛋白表达的影响

发布时间：2018-01-13 13:18

本文关键词：电针对鱼藤酮诱导的帕金森病模型大鼠黑质内自噬相关蛋白表达的影响　出处：《辽宁中医杂志》2017年09期 　论文类型：期刊论文

更多相关文章： 帕金森病 电针 TH LC-Ⅱ P α-syn

【摘要】：目的:探讨自噬—溶酶体途径在针刺防治帕金森病(Parkinson's disease,PD)中的作用。方法:雄性SD大鼠随机分为正常组、假手术组、模型组、电针预处理组、电针治疗7 d组、电针治疗14 d组、电针治疗21 d组、电针治疗28 d组,每组15只。采取颈背部皮下注射鱼藤酮法制备PD模型。电针治疗组取穴"太冲""风府",每次治疗20 min,每天1次,疗程分别为7 d、14 d、21 d、28 d。电针预处理组电针治疗7 d后再造模。观察各组大鼠行为学改变并进行评分。造模后除去死亡与体质量过轻过重及外观、行为学未达标的老鼠,每组均为10只。运用Western blot检测黑质酪氨酸羟化酶(TH)、微管相关膜蛋白轻链3(LC3-II)、自噬底物蛋白P62和α-突触核蛋白(α-syn)的表达。结果:模型组大鼠出现了毛发变黄变粗、弓背明显、运动缓慢迟钝、拒捕减少等异常行为学表现,而治疗组和预处理组以上表现程度明显减轻。与正常组和假手术组对比,模型组LC3-II、P62、α-syn的表达均明显增加(P0.01),TH表达明显下降(P0.01)。与模型组对比,电针治疗组和预处理组LC3-II的表达水平增加明显(P0.01);TH表达增强(P0.01或P0.05),P62、α-syn蛋白表达均明显下降(P0.01)。结论:电针防治帕金森病的机制可能与针刺能够改善自噬功能障碍,促进PD模型大鼠体内具有细胞毒性作用α-syn的降解有关。
[Abstract]:Objective: to investigate the role of autophagy lysosome pathway in the prevention and treatment of Parkinson's disease (PD) by acupuncture. Methods: male Sprague-Dawley rats were randomly divided into normal group. Sham operation group, model group, electroacupuncture pretreatment group, electroacupuncture treatment group, electroacupuncture treatment group, electroacupuncture treatment group, electroacupuncture treatment group, electroacupuncture treatment group, electroacupuncture treatment group for 28 days. The PD model was established by subcutaneous injection of rotenone into the neck and back of 15 rats in each group. In the electroacupuncture group, the acupoints "Taochong" and "Fengfu" were taken for 20 minutes, once a day, for 7 days. The rats in the electroacupuncture preconditioning group were treated with electroacupuncture for 7 days and then reconstructed the model. The behavioral changes in each group were observed and scored. After the establishment of the model, the death and body weight were removed and the appearance and appearance of the rats were removed. Western blot was used to detect tyrosine hydroxylase in substantia nigra and microtubule-associated membrane protein light chain (3LC-III). Expression of autophagy protein P62 and 伪 -synsyn.Results: in the model group, the hair turned yellow and thickened, the back of the bow was obvious, and the movement was slow and slow. The abnormal behavioral manifestations such as resisting arrest and reducing arrest were significantly reduced in treatment group and preconditioning group. Compared with normal group and sham operation group, LC3-IIP 62 in model group. The expression of 伪 -syn significantly increased the expression of P0.01TH decreased significantly, compared with the model group. The expression level of LC3-II in electroacupuncture treatment group and pretreatment group was significantly increased (P 0.01). Th expression was enhanced by P0.01 or P0.05 P62. Conclusion: the mechanism of electroacupuncture in the prevention and treatment of Parkinson's disease may be related to the improvement of autophagy dysfunction by acupuncture. It is related to promoting the degradation of 伪-syn in PD model rats.
【作者单位】：湖北中医药大学针灸骨伤学院/针灸治未病湖北省协同创新中心;
【基金】：国家自然科学基金项目(81403456,81473788) 武汉市科学技术局高新技术产业科技创新团队计划项目(2014072704021262) 针灸治未病湖北省协同创新中心项目(HBPCIC-2016-003)
【分类号】：R245.97;R-332
【正文快照】： 帕金森病(Parkinson's disease,PD),又称为震颤麻痹,与小脑萎缩症、肌肉萎缩性侧索硬化症、多发性硬化症等疾病同属神经退行性疾病范畴。其主要临床表现为静止性震颤、肌张力增高、运动迟钝缓慢、姿势不稳。PD最具典型的病理学特征为黑质多巴胺(Do-pamine,DA)能神经元变性坏死

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