中药复方藤菔降压片治疗高血压病肝阳上亢证临床疗效及其组分对血管内皮细胞自噬的影响

发布时间：2018-01-16 09:23

本文关键词：中药复方藤菔降压片治疗高血压病肝阳上亢证临床疗效及其组分对血管内皮细胞自噬的影响　出处：《山东中医药大学》2016年硕士论文　论文类型：学位论文

【摘要】：目的:通过临床研究,评价藤菔降压片治疗高血压病肝阳上亢证的有效性和安全性,并通过体外实验探究藤菔降压片有效组分对血管内皮细胞自噬水平的影响,从而揭示其保护血管内皮细胞的作用机理。方法:临床研究:将60例符合纳入要求的高血压病肝阳上亢证患者,随机分为试验组和对照组,试验组给予藤菔降压片口服,对照组给予牛黄降压丸口服,治疗疗程为4周,观察治疗前后血压及症状改善情况,评价其安全性。体外实验:采用组织贴块法,原代培养血管内皮细胞,通过氧化低密度脂蛋白的氧化应激反应,诱导对数生长期的血管内皮细胞发生自噬,并进行药物干预,观察其对自噬水平的影响及对细胞的保护作用。结果:临床研究:患者血压较治疗前下降明显,中医证候积分明显改善,均具有统计学意义(p0.05)。两组治疗过程中均未出现明显不良反应、过敏反应等危险事件。体外实验:钩藤总生物碱和芥子碱硫氰酸盐能够明显上调氧化低密度脂蛋白诱导的血管内皮细胞自噬水平,减轻氧化应激的损害作用。结论:中药复方藤菔降压片治疗高血压病肝阳上亢证患者具有明显的疗效,能够有效地控制血压并改善患者临床症状,并且安全性高、未见不良反应。其作用机制可能与钩藤总生物碱和芥子碱硫氰酸盐通过上调血管内皮细胞的自噬水平以保护血管内皮细胞免受ox-LDL损伤有关。
[Abstract]:Objective: to evaluate the efficacy and safety of sulforaphane in the treatment of hyperactivity of liver-yang in patients with essential hypertension, and to explore the effect of effective components of sulforaphane on the level of autophagy of vascular endothelial cells in vitro. Methods: clinical study: 60 patients with hyperactivity of liver yang were randomly divided into experimental group and control group. The experimental group was treated with sulforaphane tablets and the control group with Niuhuang Jiangya pills for 4 weeks. The blood pressure and symptom improvement were observed before and after treatment and the safety was evaluated. In vitro experiment: tissue patch method was used. Primary cultured vascular endothelial cells (VECs) were induced to autophagy in logarithmic growth phase by oxidative stress reaction of oxidized low density lipoprotein (LDL) and drug intervention was carried out. To observe the effect on autophagy level and its protective effect on cells. Results: clinical study: the blood pressure of patients was significantly lower than that before treatment, and the integral of TCM syndromes was obviously improved. There was no significant adverse reaction in both groups. In vitro experiments: total alkaloids and erucidine thiocyanate could significantly upregulate the level of autophagy induced by oxidized low density lipoprotein (LDL). Conclusion: traditional Chinese medicine compound sulforaphane is effective in treating patients with hyperactivity of liver yang in hypertension. It can effectively control blood pressure and improve the clinical symptoms of patients with hyperactivity of liver yang. And safe. The mechanism may be related to the up-regulation of autophagy of vascular endothelial cells by total alkaloids and erucidine thiocyanates in order to protect vascular endothelial cells from ox-LDL damage.
【学位授予单位】：山东中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R259

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