不同经穴组合针刺对失眠大鼠下丘脑生物钟基因Clock和Bmal 1表达的影响

发布时间：2018-01-21 13:10

本文关键词： 循经选穴针刺失眠昼夜节律生物钟基因 Bmal 基因下丘脑　出处：《针刺研究》2017年05期 　论文类型：期刊论文

【摘要】：目的:观察不同经穴组合针刺对失眠大鼠下丘脑生物钟基因Clock和Bmal 1表达的影响,探索循经选穴对腧穴配伍效应的影响。方法:SD雄性大鼠随机分为空白组(n=12)、模型组(n=8)、百会+神门组(n=12)、百会+三阴交组(n=11)、百会+非经非穴组(n=10)。连续2d腹腔注射对氯苯丙氨酸(PCPA)混悬液(500mg/kg)建立失眠模型。百会+神门组、百会+三阴交组、百会+非经非穴组每日针刺1次,每次30min,连续7d。治疗后比较各组大鼠睡眠潜伏期及睡眠时间;实时荧光定量PCR法检测下丘脑腹外侧视前区(VLPO)与视交叉上核(SCN)的Clock及Bmal 1mRNA的表达水平。结果:与空白组比较,模型组睡眠潜伏期延长、睡眠时间减少(P0.05),VLPO区与SCN内的Clock及Bmal 1mRNA表达水平均降低(P0.01)。与模型组比较,百会+神门组睡眠时间延长(P0.05);3个治疗组睡眠潜伏期均缩短(P0.05),VLPO区与SCN内Bmal 1mRNA表达水平均升高(P0.05);百会+神门组和百会+三阴交组VLPO区与SCN内Clock mRNA表达水平均升高(P0.01)。与百会+神门组比较,百会+三阴交组和百会+非经非穴组睡眠时间减少(P0.05)。与百会+三阴交组比较,百会+神门组VLPO区与SCN内Clock及Bmal 1mRNA表达水平均升高(P0.01,P0.05);百会+非经非穴组VLPO区与SCN内Clock及Bmal1mRNA表达水平均降低(P0.01,P0.05)。结论:针刺"百会"配伍"神门"穴可延长失眠大鼠的睡眠时间并缩短潜伏期,提高VLPO区与SCN内生物钟基因Clock及Bmal 1表达水平,循经选穴可以提高腧穴配伍效应。
[Abstract]:Objective: to observe the effect of acupuncture combined with different meridian points on the expression of Clock and Bmal 1 in hypothalamus of insomnia rats. Methods male SD rats were randomly divided into blank group (n = 12), model group (n = 8) and Baihui Shenmen group (n = 12). The Baihui Sanyinjiao group (Sanyinjiao group). The model of insomnia was established by intraperitoneal injection of PCPA suspension (500 mg / kg) for 2 days in the Baihui Shenmen group, Baihui Shenmen group and Baihui Sanyinjiao group. The rats in the Baihui non-meridian acupoint group were treated with acupuncture once a day for 30 minutes for 7 days. The sleep latency and sleep time of the rats in each group were compared after treatment. Real-time fluorescence quantitative PCR assay for the detection of hypothalamic ventrolateral preoptic area (VLAO) and supraoptic chiasma (SCN). Results: compared with the blank group, the expression level of Clock and Bmal 1 mRNA in the control group was higher than that in the control group. In the model group, the sleep latency was prolonged and the sleep time was decreased (P0.05). The expression levels of Clock and Bmal 1 mRNA in VLPO and SCN were lower than those in model group. Compared with model group, the sleep time of Baihui Shenmen group was longer than that of model group. In the three treatment groups, the sleep latency was shortened and the expression level of Bmal 1 mRNA in SCN and P0.05 were increased. The expression levels of Clock mRNA in the VLPO region and SCN in the Baihui Shenmen group and Baihui Sanyinjiao group were higher than those in the Baihui Shenmen group and the Baihui Shenmen group. The sleep time of the Baihui Sanyinjiao group and the Baihui non-meridian non-acupoint group was decreased by P0.050.Compared with the Baihui Sanyinjiao group. The expression levels of Clock and Bmal 1 mRNA in the VLPO region and SCN in the Baihui Shenmen group were significantly higher than those in the control group. The expression levels of Clock and Bmal1mRNA in the VLPO region and SCN in the non-acupoints of Baihui non-meridian group were significantly lower than those in the control group (P0.01). Conclusion: acupuncture with "Baihui" and "Shenmen" can prolong the sleep time and shorten the latency of insomnia rats. Increasing the expression of Clock and Bmal 1 in VLPO and SCN, and selecting acupoints along the meridians could improve the compatibility of acupoints.
【作者单位】：湖南中医药大学第一附属医院;湖南中医药大学中西医结合学院;湖南中医药大学针灸推拿学院;
【基金】：国家重点基础研究发展计划“973”中医专项(No.2014CB 543102)
【分类号】：R245
【正文快照】： 美国睡眠医学会(AASM)2014年发布了第3版睡眠疾病国际分类指南(international classifica-tion of sleep disorders,third edition,ICSD-3),提出失眠(insomnia)分为入睡困难、夜间间歇性失眠、早醒或这些问题的任何组合[1]。现代研究发现,调控生物节律的生物钟基因与睡眠关系

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