山药多糖的提取分离及活性初步研究.pdf 免费在线阅读前50页

  本文关键词：山药多糖的提取分离及活性初步研究，由笔耕文化传播整理发布。

网友1318384917近日为您收集整理了关于山药多糖的提取分离及活性初步研究的文档，希望对您的工作和学习有所帮助。以下是文档介绍：江苏大学硕士学位论文山药多糖的提取分离及活性初步研究姓名:高英春申请学位级别:硕士专业:生药学指导教师:陈钧20090604江苏大学硕士学位论文摘要山药是一种多年生缠绕草质藤本植物,为薯蓣科薯蓣属植物薯蓣(DioscoreaoppositeThunb.)的块茎,具有健脾、补肺、固肾等功效。多糖是山药中的主要活性成分,具有增强免疫功能,降血糖、降血脂、抗氧化、抗衰老等多种生理活性。本课题以江苏东台产山药为研究对象,对其中的多糖类成分进行了研究,为开发利用山药资源,提供理论依据。本论文通过单因素和正交试验优化了山药片粗多糖的提取条件,确定了山药片粗多糖的实验室最佳提取条件为:提取温度100*C,提取时间为3h,料液比为1:12,pH为8,提取液真空浓缩至原体积的1/5,所用95%乙醇的体积为多糖浓缩液体积的3倍。采用该工艺提取的山药粗多糖,粗多糖得率为11.58%,苯酚一硫酸法测定山药多糖含量为67.03%。以蛋白脱除率和多糖保留率为指标,对山药粗多糖中蛋白质脱除方法(盐酸法、三***乙酸法、胰蛋白酶法)进行了比较,确定最佳方法为胰蛋白酶法,最佳条件为:浓度lmg/ml山药粗多糖溶液10ml,酶用量0.5ml,酶解时间45 min,酶解温度37℃。在此条件下,蛋白脱除率为88.7%,多糖保留率为93.1%。山药多糖经除蛋白、除色素、流水透析后经DEAE--52纤维素层析柱,得到RDPd.I、RDPd.II两种组分,再分别过Sephadex G.100凝胶柱进一步纯化,得到RDPs.I、RDPs.II两组分,经高效液相检测,呈单一对称峰,表明达到一定的均一纯度。采用UV、IR、HPLC等现代分析手段,分析了山药多糖的两种组分RDPs.I、RDPs.II。紫外光谱在260nm一600nm处无明显的吸收,表明经分离纯化的山药多糖的两种组分RDPs-I、RDPs—II均不含核酸、蛋白质,红外光谱显示有糖的特征吸收峰。RDPs.I、RDPs.II红外图谱中未见810cm。1和870cm‘1处的吸收峰,可能均不含甘露糖。HPLC测定分子量结果为:RDPs.1分子量为7.62×103 Da,RDPs.11分子量为6.74x10a Da。论文对山药粗多糖的药理活性进行了初步研究,山药粗多糖对正常小鼠的体重、血糖值、肝糖原含量没有影响。山药粗多糖具有降低糖尿病小鼠血糖、血脂的作用,能够提高糖尿病小鼠葡萄糖耐受能力,促进肝糖原合成,增加肝糖原含量。体外抗肿瘤试验表明:山药多糖纯化后得到的RDPs.I、RDPs.II在浓度较低时,对人肝癌细胞HepG2呈现促进作用;RDPs—II对HepG2细胞有一定的抑制作用。江苏大学硕士学位论文关键词: 山药多糖提取纯化降血糖抗肿瘤Ⅱ江苏大学硕士学位论文ABSTRACTRhmoma dioscoreae is one kind of herbaceous perennial vine,the stem belongs toDioscorea opposite Thunb.It is good for spleen,lung and kindey.harides is the mainactive ingredient in Rhizoma dioscoreae,which has the function of enhancing immunefunction,redudng blood sugar and blood fat,anti—oxidation;anti-aging and SO on.This articlechooses the Rhizoma dioscoreae produced in DongTai Jiang Su Pmvmce,and study harides in order to provide the theorical evidence to develop the source of Rhizomadioscoreae.The article studies the extraction technology of Rhmoma dioscoreae halides.Single factor test and orthogonal experiment design method L9(34)were applied.The optimalextraction technology of Rhizoma dioscoreae pieces is with water 12 times for 3H,100"C,pH=8,harides solution concentrate to 1/5,the volume of 95%ethanol is 3 times morethan harides concentrated liquid.The extraction rate of harides of Rhizomadioscoreae is 11.58%,and halide content is 67.03%.Three methods ale used to remove protein of Rhizoma dioscoreae harides.Theoptimal factor for removing protein is:RDCP solution 1 0ml(1mg/m1),TPS 0.5ml,posing time 45min and posing temperature 37"(2.The removing rate for protein is88.7%and retaining rate for harides is 93.1%.The Rh娩oma dioscoreae harides is done by dismissing protein and pigment,flow dialysis and DEAE一52 cellulose column chromatography,the Rhmoma harides Was separated 2 parts,RDPd·I and RDPd-II.And then they purified bySephadex G-100 column chromatography,RDPs-I and RDPs—II are received.They ale singlesymmetrical peaks detected by HPLC.It concluded that the ponents of Rhmomadioscoreae harides are pure.The article studies the RDPs—I and RDPs—II by uv,IR,HPLC and GC-MS.Theyshowed characteristic absorption peaks on UV and IR.It cannot see

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  本文关键词：山药多糖的提取分离及活性初步研究，由笔耕文化传播整理发布。