电针调控新西兰兔骨关节炎性疼痛及对脊髓IL-17、IL-17R表达的影响

发布时间：2018-03-03 16:29

  本文选题：电针　切入点：新西兰兔　出处：《重庆医学》2017年17期 　论文类型：期刊论文

【摘要】：目的研究电针是否通过抑制脊髓内白细胞介素(IL)-17及IL-17R的表达来调控新西兰兔骨关节炎性疼痛。方法新西兰兔随机分为假手术组、炎症模型组、假手术+IL-17组、炎症+抗IL-17组、炎症+电针组、炎症+假电针组,每组8只,4%木瓜蛋白酶构建兔骨关节炎模型,IL-17及IL-17抗血清采用椎管内注射,电针组选择电针刺激足三里(30min,2 Hz连续波,1~2mA),假电针组采用非穴位刺激,测定兔痛阈,采用实时荧光定量PCR(RT-qPCR)检测兔脊髓组织IL-17、IL-17R的mRNA表达,Western bolt检测IL-17、IL-17R蛋白表达。结果在构建骨关节炎模型后新西兰兔痛阈显著下降(P0.05),椎管注射IL-17后可以显著降低假手术新西兰兔痛阈(P0.05),而椎管注射IL-17抗血清后可明显增加骨关节炎模型兔痛阈值。选择足三里电针刺激可显著提升兔痛阈(P0.05)。骨关节炎模型组IL-17、IL-17R的mRNA及蛋白表达显著增加(P0.05),而电针刺激后其表达显著下降(P0.05)。结论新西兰兔骨关节炎性疼痛发生可能涉及脊髓IL-17异常表达,而电针可能通过抑制脊髓IL-17、IL-17R的表达来调控骨关节炎性疼痛。
[Abstract]:Objective to study whether electroacupuncture regulates osteoarthritis pain in New Zealand rabbits by inhibiting the expression of interleukin-17 and IL-17R in spinal cord. Methods New Zealand rabbits were randomly divided into three groups: sham operation group, inflammatory model group, IL-17 group and anti-inflammatory IL-17 group. Inflammatory electroacupuncture group, inflammatory pseudoelectroacupuncture group, 8 rabbits in each group with 4% papain to construct rabbit osteoarthritis model, IL-17 and IL-17 antiserum were injected into the spinal canal. Electroacupuncture group (EA group) was used to stimulate Zusanli (Zusanli) for 30 min (2 Hz) and sham electroacupuncture group (sham group) was used to measure the pain threshold of rabbits by non-acupoint stimulation. The expression of IL-17hIL-17R in rabbit spinal cord tissue was detected by real-time fluorescence quantitative PCR- RT-qPCR.The expression of IL-17hIL-17R protein in rabbit spinal cord tissue was detected by Western bolt. Results the pain threshold of New Zealand rabbit decreased significantly after the establishment of osteoarthritis model (P 0.05), and the spinal canal injection of IL-17 could significantly reduce the new sham-operation. The pain threshold of New Zealand rabbit was P0.05A, and the pain threshold of rabbit model of osteoarthritis was significantly increased after injecting IL-17 antiserum into the spinal canal. Electroacupuncture stimulation of Zusanli could significantly increase the pain threshold of rabbit. The expression of mRNA and protein of IL-17 and IL-17R in osteoarthritis model group increased significantly (P 0.05). Conclusion the occurrence of osteoarticular inflammatory pain in New Zealand rabbits may involve abnormal expression of IL-17 in spinal cord. Electroacupuncture may regulate osteoarthritis pain by inhibiting the expression of IL-17 and IL-17 R in spinal cord.
【作者单位】： 西南医科大学附属医院康复医学科;四川省泸州市人民医院康复医学科;
【基金】：四川省中医药管理局青年中医药课题基金资助(2014K055)
【分类号】：R245
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本文编号：1561804