针药结合对盆腔炎性疾病后遗症模型大鼠IL-4及IL-10表达的影响

发布时间：2018-03-13 18:04

本文选题：盆腔炎性疾病后遗症　切入点：SPID　出处：《黑龙江中医药大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:1、成功建立盆腔炎性后遗症大鼠模型,观察大鼠不同时期的病理变化及血液指标的改变,确定盆舒饮及针刺治疗的时间。2、通过对比各组对SPID大鼠治疗观察病灶的病理、血液指标的变化,为临床治疗提供依据。3、从"正虚邪恋"的角度论述SPID的基本病机,"瘀血阻滞"为其病理结局。并结合西医(IL-4、IL-10指标的变化)探讨针药结合的治疗效果。方法:1、采用机械损伤+细菌感染造模法建立SPID大鼠模型,于造模后第15天查看病灶情况及取子宫组织做病理形态学的观察。2、分别观察6组大鼠(空白组、模型组、丹黄祛瘀胶囊组、盆舒饮组、针刺组、盆舒饮+针刺组)的病理情况及血液中IL-4、IL-10表达情况。结果:1、成功建立SPID大鼠模型,病理显示造模15天,子宫病理显示:内膜上皮细胞部分脱落或增生,间质细胞被纤维组织取代,腺体变形,腺体管腔壁空泡样改变。2、模型组大鼠血液中IL-4及IL-10表达较正常组均有所升高,差异有极显著统计学意义(P0.01)。各治疗组大鼠血IL-4及IL-10浓度表达较模型组均有所降低,但未达到正常水平。针药结合组血IL-4及IL-10浓度接近空白组,差异无统计学意义(P0.05),均优于其它治疗组。IL-4表达方面,针药结合组优于各治疗组,有极显著统计学意义(P0.01);与丹黄组比较,盆舒饮组和针刺组治疗效果较好,盆舒饮组极显著统计学意义(P0.01),针刺组具有显著统计学意义(P0.05)。IL-10表达方面,针药结合组优于各治疗组,与针药结合组比较,盆舒饮组及丹黄组有极显著的统计学意义(P0.01),针刺组具有显著的统计学差异(P0.05);针刺组优于丹黄组具有极显著的统计学意义(P0.01),丹黄组优于盆舒饮组差异具有显著的统计学意义(P0.05)。结论:通过机械损伤加混合细菌造模法可成功建立SPID大鼠模型。在SPID的治疗上,针药结合疗效优于其它单一方法治疗组,可减少炎细胞浸润,并减轻组织粘连,促进组织修复;能够下调模型大鼠SPID血液中的IL-4及IL-10浓度,缓解盆腔炎症,改善盆腔粘连。
[Abstract]:Objective: to establish the rat model of pelvic inflammatory sequelae successfully, observe the pathological changes and the changes of blood indexes in different periods of rats, determine the time of treatment of Pan-Shuyin and acupuncture, and observe the pathological changes of the lesions by comparing the treatment of each group with that of SPID rats. Changes in blood indicators, In order to provide the basis for clinical treatment, this paper discusses the basic pathogenesis of SPID from the angle of "positive deficiency and evil love", and "blood stasis block" as its pathological outcome. In combination with the changes of IL-4 / IL-10 index in western medicine, the therapeutic effect of acupuncture and medicine combination is discussed. The SPID rat model was established by the method of mechanical injury bacterial infection. On the 15th day after the establishment of the model, the lesions were examined and the uterine tissues were taken for pathomorphological observation. The rats in 6 groups (blank group, model group, Danhuang Quyu capsule group, Pan-Shuyin group and acupuncture group) were observed respectively. Results: SPID rat model was established successfully. The pathological results showed that the endometrial epithelial cells were partly shed or proliferated. The interstitial cells were replaced by fibrous tissue, the gland deformed, the wall vacuolation of glandular lumen changed. The expression of IL-4 and IL-10 in the blood of the model group was higher than that of the normal group. The expression of serum IL-4 and IL-10 in each treatment group was lower than that in the model group, but did not reach the normal level. The serum IL-4 and IL-10 concentrations in the acupuncture and medicine combination group were close to the blank group. The difference was not statistically significant (P 0.05), which was better than that of other treatment groups in the expression of IL-4, the combination of acupuncture and medicine was superior to the other treatment groups (P 0.01), and compared with the Danhuang group, the treatment effect of the two groups was better than that of the control group, and that of the acupuncture group was better than that of the other treatment groups. The expression of P0.05 + IL-10 in the acupuncture group was significantly higher than that in the treatment group, and was significantly higher in the acupuncture group than in the acupuncture and medicine combination group, and was significantly higher in the acupuncture group than in the acupuncture and medicine combination group, and the expression of IL-10 in the acupuncture group was significantly higher than that in the acupuncture and medicine combination group. There were very significant statistical significance in the group of Pan-Shuyin and Danhuang, there was significant difference between the acupuncture group and the acupuncture group (P 0.05), the acupuncture group had a very significant statistical significance than the group of Danhuang, and the group of Danhuang was superior to the group of Peshuyin, and the difference of the group of acupuncture was more significant than that of the group of Pan-Shuyin. Conclusion: SPID rat model can be successfully established by mechanical injury and mixed bacterial modeling. The therapeutic effect of acupuncture combined with medicine is better than other single treatment group, can reduce inflammatory cell infiltration, reduce tissue adhesion, promote tissue repair, can down-regulate the concentration of IL-4 and IL-10 in SPID blood of model rats, alleviate pelvic inflammation and improve pelvic adhesion.
【学位授予单位】：黑龙江中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R271.9;R-332

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