针刺结合益气祛风汤对慢性ACD小鼠病变组织中Fas及FasL表达的影响

发布时间：2018-03-28 12:33

  本文选题：益气祛风汤　切入点：慢性ACD　出处：《辽宁中医药大学》2016年硕士论文

【摘要】：目的:建立慢性ACD小鼠模型,并用针刺结合益气祛风汤的方法对慢性ACD模型小鼠进行治疗,进而观察其对小鼠组织形态学的影响,并探讨其对慢性ACD模型小鼠耳病变皮肤组织中Fas及Fas L表达的影响,为临床能够更好地应用针刺结合的方法治疗变应性接触性皮炎提供可靠而客观的依据。材料和方法:1.实验材料(1)实验动物:60只健康的雄性昆明种小鼠。(2)实验用药:通过灌胃给药方式,中药组给予益气祛风汤颗粒剂悬浊液;西药组给予氯雷他定片悬浊液;针药组给予益气祛风汤颗粒剂悬浊液;空白组及模型组均给予等量蒸馏水。2.研究方法(1)造模方法:采用局部皮肤涂抹2,4-二硝基氯苯(2,4-DNCB)的方法制备小鼠的慢性ACD动物模型。(2)通过观察针刺结合益气祛风汤对慢性ACD模型小鼠病变耳重量差的影响,进而评价其对慢性ACD小鼠的抑制作用。(3)采用免疫组化的方法,进行对慢性ACD模型小鼠病变皮肤组织中Fas/Fas L的含量进行检测,研究其表达的变化,进一步探究针刺结合益气祛风汤对慢性ACD小鼠的抑制作用。结果:1.针刺结合益气祛风汤可明显下调慢性ACD模型小鼠病变耳的重量差值:慢性ACD模型组的小鼠耳重量差值明显高于空白组(P0.01),针药组及氯雷他定组的耳重量差值明显低于模型组(P0.05),针刺及中药组的耳重量差值与模型组相比均无显著性差异(P0.05);针药组与氯雷他定组之间无显著性差异(P0.05)。2.针刺结合益气祛风汤可明显上调慢性ACD模型小鼠耳病变皮肤组织中Fas/Fas L的表达:慢性ACD模型组的小鼠耳病变皮肤组织中的Fas、Fas L的表达均增高;针药组、针刺组及中药组小鼠耳病变皮肤组织中Fas、Fas L的表达均明显上调;氯雷他定组对小鼠耳病变皮肤组织中Fas、Fas L的表达无明显影响,且针刺结合益气祛风汤组与氯雷他定组之间无明显差异。结论:1.针刺结合益气祛风汤对慢性ACD模型小鼠有抑制作用,且能降低其病变耳的重量差值;2.针刺结合益气祛风汤能明显上调慢性ACD模型小鼠的耳病变皮肤组织中Fas和Fas L的表达。
[Abstract]:Objective: to establish the chronic ACD mouse model and treat the chronic ACD model mice with acupuncture combined with Yiqi Qufeng decoction, and then observe its effect on the histomorphology of the mice. To investigate the effect of Fas and Fas L on the expression of Fas and Fas L in the skin of chronic ACD model mice. To provide a reliable and objective basis for clinical application of acupuncture combined with acupuncture in the treatment of allergic contact dermatitis. Materials and methods: 1) Experimental animals: 60 healthy male Kunming mice. By gavage, The traditional Chinese medicine group was treated with Yiqi Qufeng decoction granule suspension; the western medicine group with loratadine tablet suspension liquid; the acupuncture and medicine group with Yiqi Qufeng decoction granule suspension; The blank group and the model group were given the same amount of distilled water. 2. The research method was 1) the method of making model: the chronic ACD animal model of mice was made by using local skin smearing with 2NCB-dinitrochlorobenzene 24-DNCB.) by observing acupuncture combined with Yiqi Qufeng decoction, the animal model of chronic ACD in mice was established by observing acupuncture combined with Yiqi Qufeng decoction. The effect on the weight difference of diseased ears in chronic ACD model mice, To evaluate its inhibitory effect on chronic ACD mice, immunohistochemical method was used to detect the content of Fas/Fas L in the diseased skin tissue of chronic ACD model mice, and to study the changes of its expression. To further explore the inhibitory effect of acupuncture combined with Yiqi Qufeng decoction on chronic ACD mice. Results 1. Acupuncture combined with Yiqi Qufeng decoction could significantly decrease the weight difference of diseased ears in chronic ACD model mice: ear weight of chronic ACD model group. The volume difference was significantly higher than that of the blank group (P 0.01), the difference of ear weight between the acupuncture group and the loratadine group was significantly lower than that of the model group (P 0.05), and there was no significant difference in the ear weight difference between the acupuncture group and the traditional Chinese medicine group compared with the model group (P 0.05), and the difference between the acupuncture group and the loratadine group was not significant (P 0.05). There was no significant difference between them. Acupuncture combined with Yiqi Qufeng decoction could obviously upregulate the expression of Fas/Fas L in the ear lesion skin of chronic ACD model mice. The expression of Fas-Fas L in the ear lesion skin tissue of chronic ACD model group was all increased. The expression of Fas-Fas L in ear lesion skin of mice in acupuncture group, acupuncture group and traditional Chinese medicine group were all upregulated obviously, while the expression of Fas-Fas L in mouse ear lesion skin tissue was not affected by loratadine group. There was no significant difference between acupuncture combined with Yiqi Qufeng decoction group and loratadine group. Conclusion: 1. Acupuncture combined with Yiqi Qufeng decoction can inhibit chronic ACD model mice. Acupuncture combined with Yiqi Qufeng decoction could obviously upregulate the expression of Fas and Fas L in the skin of chronic ACD model mice.
【学位授予单位】：辽宁中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R245
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本文编号：1676345