电针“印堂”“天枢”穴对肠易激综合征大鼠行为学及结肠辣椒素受体的影响

发布时间：2018-04-02 11:21

本文选题：肠易激综合征　切入点：电针　出处：《针刺研究》2017年02期

【摘要】：目的:观察电针"印堂""天枢"穴对肠易激综合征(IBS)大鼠行为学及结肠辣椒素受体(TRPV 1)表达的影响,探讨电针不同主治功能的穴位对身心疾病不同症状疗效的差异及其相关机制。方法:Wistar新生大鼠32只随机分为空白对照组、模型对照组、印堂组、天枢组。除空白对照组外,均采用新生期母子分离加醋酸灌肠并联合结直肠扩张刺激法制备IBS大鼠模型。大鼠9周龄时印堂和天枢组分别给予电针干预,每次20min,隔日1次,共5次。采用腹部回撤反射对大鼠进行内脏敏感性评估,观察第1次收缩波潜伏期及90s内收缩波个数;采用旷场实验评估大鼠情绪心理行为,观察其水平及垂直运动次数;采用免疫组织化学法检测结肠组织TRPV 1的表达。结果:与空白对照组比较,模型对照组第1次收缩波潜伏期明显缩短(P0.01),收缩波个数明显增多(P0.01),水平运动次数以及垂直运动次数均明显减少(P0.01),结肠内TRPV 1免疫反应物阳性表达水平增强(P0.05)。与模型对照组比较,天枢组及印堂组第1次收缩波潜伏期延长及90s内收缩波个数明显减少(P0.01,P0.05),天枢组垂直运动次数增多(P0.05),印堂组的垂直运动次数及水平运动次数均明显增多(P0.01),天枢组及印堂组结肠TRPV 1免疫反应物阳性表达显著下降(P0.01,P0.05)。与印堂组比较,天枢组TRPV 1免疫反应物阳性表达显著下降(P0.01)。结论:电针"印堂""天枢"穴均可明显缓解IBS大鼠的腹痛并改善精神情绪的异常,电针"印堂"穴能更好地缓解模型大鼠抑郁样情绪行为,而电针"天枢"穴治疗腹痛效果较佳,二者对结肠内TRPV 1免疫反应物阳性表达的影响存在明显差异。
[Abstract]:Objective: to observe the effect of electroacupuncture on behavior and expression of colonic capsaicin receptor TRPV1 in rats with irritable bowel syndrome (IBS).To explore the effect of electroacupuncture on different symptoms of physical and mental diseases and its related mechanism.Methods Thirty-two newborn Wistar rats were randomly divided into three groups: blank control group, model control group, Yantang group and Tianshu group.In addition to the blank control group, IBS rat model was established by the method of neonatal mother and child separation and acetic acid enema combined with colorectal dilatation stimulation.The rats were treated with electroacupuncture for 20 min, once every other day, for 5 times, at the age of 9 weeks in Yantang group and Tianshu group.The visceral sensitivity of rats was evaluated by abdominal withdrawal reflex, the latency of the first systolic wave and the number of systolic waves in 90s were observed, the emotional psychological behavior of rats was evaluated by open field experiment, and the horizontal and vertical movement times were observed.Immunohistochemical method was used to detect the expression of TRPV 1 in colon tissue.Results: compared with the blank control group,In the model control group, the latency of the first contraction wave shortened significantly, the number of the contraction wave increased significantly, the number of horizontal and vertical movements decreased significantly, and the positive expression of TRPV 1 immunoreactive substance increased in the colon.Compared with the model control group,In Tianshu group and Yantang group, the latency of the first systolic wave prolonged and the number of contraction waves decreased significantly in 90 s. The number of vertical motion increased in Tianshu group, and the number of vertical motion and horizontal motion increased significantly in Tianshu group, and the number of vertical motion and horizontal motion in Yantang group increased significantly (P 0.01), and in Tianshu group and in Tianshu group, the number of vertical motion and horizontal motion increased significantly; andThe positive expression of TRPV 1 immunoreactive substance in the colon of Yintang group decreased significantly.Compared with Yintang group, the positive expression of TRPV 1 immunoreactive substance in Tianshu group decreased significantly (P 0.01).Conclusion: electroacupuncture at "Yantang" and "Tianshu" can obviously relieve abdominal pain and improve mental mood abnormality in IBS rats. Electroacupuncture at "Yantang" can better relieve depressive emotional behavior in model rats.The effect of electroacupuncture on abdominal pain was better. There was significant difference between them on the expression of TRPV 1 immunoreactive substance in colon.
【作者单位】：北京中医药大学针灸推拿学院;
【基金】：国家自然科学基金课题(No.81473776)
【分类号】：R245.97

【相似文献】