补髓生血颗粒对慢性髓劳病患者整合素连接激酶（ILK）影响的研究

发布时间：2018-04-03 03:32

本文选题：慢性髓劳病　切入点：慢性再障　出处：《黑龙江中医药大学》2017年硕士论文

【摘要】：目的:通过观察慢性再生障碍性贫血(Chronic aplastic anemia,CAA,以下简称"慢性再障”,中医称"慢性髓劳病")患者服用补髓生血颗粒前后骨髓单个核细胞中整合素连接激酶(Integrin-linked kinase,ILK)蛋白表达的变化,探讨补髓生血颗粒对再障患者的ILK及造血微环境的影响,进而了解ILK与再障发生发展的关系,为探索补髓生血颗粒治疗慢性再障的机理提供依据。方法:1.收集30例应用补髓生血颗粒治疗的慢性再障(CAA)患者的骨髓;2.收集10例健康人的骨髓;3.采用Western blot法检测患者治疗前后以及与健康对照组骨髓单个核细胞ILK蛋白的表达水平。结果:1.补髓生血颗粒治疗慢性髓劳病的临床疗效达到80.00%,疗效较确切。2.慢性髓劳病患者治疗前ILK蛋白表达水平明显高于健康组,差异有统计学意义(P0.05),治疗组经补髓生血颗粒治疗后,ILK蛋白表达水平较治疗前显著下降,差异有统计学意义(P0.05)。3.治疗组治疗后的ILK蛋白表达水平仍然是高于健康对照组的,差异有统计学意义(P0.05)。结论:1.补髓生血颗粒对慢性髓劳病有一定治疗作用是可以肯定的。2.慢性髓劳患者ILK蛋白高于正常人表达水平可能参与慢性髓劳的发病过程。3.补髓生血颗粒可能通过降低慢性髓劳患者ILK蛋白的表达水平以调节整合素β1介导的造血微环境损伤,进而促进造血干/祖细胞的增殖分化以达到治疗慢性髓劳病的目的。
[Abstract]:Objective: to observe the expression of integrin linked kinase integrin-linked kinase (ILK) protein in bone marrow mononuclear cells of chronic aplastic anemia patients with chronic aplastic anemia ("chronic aplastic anemia") before and after taking "chronic myelopathy".To explore the effect of Bupuishengxue granule on ILK and hematopoietic microenvironment in patients with aplastic anemia, and to understand the relationship between ILK and the occurrence and development of aplastic anemia, and to provide the basis for exploring the mechanism of Busuishengxue granule in treating chronic aplastic anemia.Method 1: 1.The bone marrow of 30 patients with chronic aplastic anemia (CAA) treated with Bupu Shengxue granule was collected.The bone marrow of 10 healthy persons was collected.The expression of ILK protein in bone marrow mononuclear cells before and after treatment and control group was detected by Western blot assay.The result is 1: 1.The clinical curative effect of Busui Shengxue granule in treating chronic myellabour disease is 80.00g, and the curative effect is more accurate. 2. 2.The expression level of ILK protein was significantly higher in patients with chronic myellabour disease before treatment than that in healthy control group (P 0.05), and the expression level of ilk protein in treatment group was significantly lower than that before treatment (P 0.05 路3).The expression of ILK protein in the treatment group was still higher than that in the healthy control group (P 0.05).Conclusion 1.Busui Shengxue granule has certain therapeutic effect on chronic myellabour disease. 2. 2.The expression level of ILK protein in patients with chronic myelosis may be involved in the pathogenesis of chronic myelosis. 3. 3.Busui Shengxue granule may regulate integrin 尾 1-mediated hematopoietic microenvironment injury by decreasing the expression level of ILK protein in chronic pull-stress patients, and then promote the proliferation and differentiation of hematopoietic stem / progenitor cells to achieve the purpose of treating chronic myelo disease.
【学位授予单位】：黑龙江中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R259

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