壮药九龙盘的化学成分及其抗炎活性研究

发布时间：2018-05-05 00:38

本文选题：九龙盘 + 3β-angeloyloxy-7-epifutronolide　；参考：《广西中医药大学》2016年硕士论文

【摘要】：目的:研究壮药九龙盘的主要化学成分及其抗炎活性,为其药效物质基础研究和开发利用提供科学依据。方法:以乙醇渗漉法提取九龙盘药材得到乙醇总提取物,总提物用系统溶剂法分离,得到石油醚部位、乙酸乙酯部位、正丁醇部位、水部位和水不溶物。采用硅胶色谱、聚酰胺色谱、葡聚糖凝胶色谱、大孔吸附树脂色谱、制备高效液相色谱、重结晶等分离方法对石油醚部位、乙酸乙酯部位和正丁醇部位的化学成分进行分离和纯化,对单体化合物利用理化检验、MS、1H-NMR、13C-NMR、DEPT等波谱方法进行结构鉴定。采用LD50测定法测试九龙盘各部位提取物的急性毒性,并通过小鼠耳肿胀、肉芽肿、腹腔毛细血管通透性三个模型对其各部位提取物进行抗炎实验研究。结果:从九龙盘中分离得到28个化合物,鉴定了其中22个化合物的结构,分别为2-十五烷酮(1),木栓酮(2),三十烷醇(3),二十八烷酸(4),β-谷甾醇(5),豆甾醇(6),邻苯二酚(7),山嵛酸(8),对甲氧基肉桂酸(11),3β-angeloyloxy-7-epifutronolide(13),6-羟基己酸(14),柠檬黄素(15),芥酸(17),3,3'-O-二甲基鞣花酸(18),β-胡萝卜苷(19),没食子酸(20),3-甲氧基槲皮素(22),4’-甲基-棉花素(23),圣草酚(24),没食子酸乙酯(25),没食子酸甲酯(26),阿福豆苷(28)。急性毒性研究测得九龙盘提取物各部位灌胃给药对小鼠的LD50分别为58.25g/kg(总提物)、1181.01g/kg(石油醚部位)、645.65g/kg(乙酸乙酯部位)、591.56g/kg(正丁醇部位)。九龙盘各部位提取物均能明显抑制小鼠腹腔毛细血管扩张;总提物、石油醚部位能明显抑制二甲苯致小鼠耳廓肿胀的炎症、小鼠棉球肉芽肿的生成;乙酸乙酯部位和正丁醇部位对二甲苯致小鼠耳廓肿胀的炎症没有明显作用,但抑制小鼠棉球肉芽肿的生成的效果显著。结论:化合物1~4、7、8、11、13~15、17、18、20、22~26、28共19个化合物为首次从该植物中分离得到,其中1~4、7、8、11、13~15、17、18、22~26、28共18个化合物为首次从该属植物中分离得到。九龙盘各部位提取物均有一定的毒性,总提物和石油醚部位提取物对小鼠的急性、慢性炎症均有较好的治疗效果,乙酸乙酯部位和正丁醇部位提取物则对抑制小鼠的慢性炎症效果更佳。
[Abstract]:Objective: to study the main chemical constituents and anti-inflammatory activity of Jiulong plate, and to provide scientific basis for the basic research and development of its pharmacodynamic substance. Methods: the total ethanol extract was extracted by ethanol percolation method, and the total extract was separated by systematic solvent method. The petroleum ether, ethyl acetate, n-butanol, water and water insoluble substances were obtained. Silica gel chromatography, polyamide chromatography, dextran gel chromatography, macroporous adsorption resin chromatography, preparation of high performance liquid chromatography and recrystallization were used to separate petroleum ether parts. The chemical constituents of ethyl acetate and n-butanol were isolated and purified. The structure of the monomers was identified by means of MS 1H-NMR-13C-NMRDPT and other spectroscopic methods. The acute toxicity of extracts from different parts of Jiulong disk was measured by LD50 method. The anti-inflammatory effects of the extracts were studied by three models of ear swelling granuloma and celiac capillary permeability in mice. Results: 28 compounds were isolated from Jiulong pan and 22 of them were identified. They are 2-pentadecanone (1), corkone (2), triaconol (3), 28 alkanoic acid (4), 尾 -sitosterol (5), lasosterol (6), catechol (7), docosanoic acid (8), p-methoxy cinnamic acid (113 尾 -angeloyloxy-7-epifutronolide) (133 尾 -angeloyloxy-7-epifutronolide) (133) -hydroxy-caproic acid (14), limonium (15), erucidic acid (173-) 3- O- dimethylanthocyanate (18), 尾 -hulucidic acid (113 尾 -angeloyloyloxy-3-hydroxycaproic acid). Turniferin 19, Gallic acid 20, 3-methoxy quercetin, methoxyquercetin, methyloxyquercetin, methoxyquercetin, chrysophenol, ethyl gallate, methoxyquercetin, methyl gallate, methoxyquercetin, methoxyquercetin. Acute toxicity study showed that the LD50 of mice treated by gavage was 58.25g / kg (total extract 1181.01g / kg) (petroleum ether extract 6455.65g / kg) (ethyl acetate 59.56g / kg). The extract of each part of Jiulong disk could obviously inhibit the abdominal capillary dilatation of mice, the total extract and petroleum ether could obviously inhibit the inflammation of auricle swelling induced by xylene and the formation of cotton ball granuloma in mice. Ethyl acetate and n-butanol had no obvious effect on ear swelling induced by xylene in mice, but could inhibit the formation of cotton ball granuloma in mice. Conclusion: a total of 19 compounds were isolated from the plant for the first time, and 18 compounds were isolated from this genus for the first time. The extracts from all parts of the Kowloon plate were toxic to a certain extent. The total extract and petroleum ether extract had a good therapeutic effect on acute and chronic inflammation in mice. The extract of ethyl acetate and butanol had better effect on inhibiting chronic inflammation in mice.
【学位授予单位】：广西中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R29

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