壮药金钮扣质量标准研究

发布时间：2018-05-26 21:11

本文选题：金钮扣 + 质量标准　；参考：《广西中医药大学》2016年硕士论文

【摘要】：目的:对壮药金钮扣质量标准进行研究,并建立其高效液相色谱指纹图谱的方法。方法:采用四大鉴别法,即原植物鉴别法、药材性状鉴别法、显微鉴别法以及理化鉴别法,对该药进行生药学鉴别的研究;按照《中华人民共和国药典》2015年版四部通则中的方法对该药的水分、灰分等常规限量检查以及浸出物含量进行研究;依据化学成分的定性反应,对该药的化学成分进行研究;运用高效液相色谱法对壮药金钮扣药材中绿原酸成分进行含量测定以及建立该药的指纹图谱。结果:(1)金钮扣植物的茎呈绿色,并带有紫红色,有纵向条纹,叶为对生,叶片呈宽卵圆形或椭圆形,顶端短尖或稍钝,全缘或为波状钝锯齿,花黄色,为头状花序;药材性状特征为茎呈黄绿色,有纵条纹,断面类白色,中空,叶纸质,皱缩,花灰黄色,顶生或腋生;根横切面中皮层可见25~35个通气组织环列,内皮层明显;茎横切面中,可见非腺毛,有10~15个外韧型维管束环列,髓部宽广,常中空;叶横切面中,表皮可见非腺毛及气孔,主脉维管束常3束;气孔轴式为不定式;花粉粒、非腺毛、木栓组织等为该药的全草粉末特征;薄层色谱鉴别中,金钮扣供试品的薄层色谱与对照药材和对照品色谱相应的位置上,显相同的蓝色的荧光斑点。(2)初步拟定,金钮扣药材水分含量、总灰分含量及酸不溶性灰分含量不得高于15.0%、23.0%以及7.0%,水溶性浸出物的含量不得少于18.0%。(3)金钮扣中可能含有有机酸、黄酮类、植物甾醇、香豆素、三萜、内酯、酚类、挥发油等化学成分类型;金钮扣中绿原酸含量在进样量为0.0100~0.4010μg范围内与峰面积呈良好线性关系,回归方程为y=2643.72866x-11.165153(r=0.9997),平均加样回收率为102.82%(RSD=1.90%),各批次金钮扣绿原酸含量在0.0043%~0.0857%之间。(4)初步建立起壮药金钮扣药材的高效液相色谱指纹图谱,筛选出了6个共有峰。结论:通过生药学鉴别研究所得出的金钮扣的特征,稳定、可靠,可以作为金钮扣定性鉴别的依据。金钮扣药材在加工时,应注意清洁,以免带入过多的泥沙,并充分干燥,防止发霉变质,以利于贮藏。采用高效液相色谱法对金钮扣中绿原酸含量进行测定,该方法准确、简便、快速,重复性好。本研究为建立壮药金钮扣药材的质量标准奠定了实验基础,为其今后的合理开发及利用提供了科学的参考依据。
[Abstract]:Objective: to study the quality standard of Zhuang Yao Jin Niu and to establish a high performance liquid chromatographic fingerprint. Methods: the pharmacokinetic identification of the drug was studied by four methods, I. e., the original plant identification method, the medicinal material character identification method, the microscopic identification method and the physicochemical identification method. According to the methods in the four general principles of the 2015 edition of the Pharmacopoeia of the people's Republic of China, the water, ash and extract contents of the drug were studied, and the chemical constituents of the drug were studied according to the qualitative reaction of the chemical constituents. High performance liquid chromatography (HPLC) was used to determine the content of Lv Yuan acid in Jinniu medicinal material and to establish its fingerprint. Results the stems of the golden button plant were green and purplish red, with longitudinally striate stripes, the leaves were opposite, the leaves were broadly oval or elliptic, the apex was short or slightly obtuse, the whole or wave-shaped obtuse serrate, the flower yellow, was the head inflorescence. The characters of medicinal materials are yellowish green stems, vertical stripes, section white, hollow, papery leaves, shrinkage, grayish yellow flowers, terminal or axillary. It can be seen that there are 10 ~ 15 outer tough vascular bundles, the pulp is broad and often hollow. In the transverse section of the leaf, the epidermis is not glandular hair and stomata, the main vein vascular bundle is usually 3 bundles; the stomatal axis is infinitive; the pollen grain, non-glandular hair, In the identification of TLC, the TLC of the gold button test sample showed the same blue fluorescence spots in the corresponding position of the control medicine and the reference substance. The water content, total ash content and acid insoluble ash content of gold button medicinal materials shall not be higher than 15.0% and 7.0%, and the content of water soluble extracts shall not be less than 18.0%. The gold button may contain organic acids, flavonoids, phytosterols, coumarins, triterpenes, lactones, etc. Phenols, volatile oil and other chemical constituents, the content of Lv Yuan acid in gold button showed a good linear relationship with the peak area in the range of 0.0100 渭 g, 0.4010 渭 g. The regression equation was yangz2643.72866x-11.165153rn 0.99970.The average recovery rate was 102.82RSD1.900.The Lv Yuan acid content of each batch of gold buttons was between 0.0043 and 0.0857%. The HPLC fingerprint of Jinjiu was preliminarily established, and six common peaks were screened out. Conclusion: the characteristics of gold buttons obtained by pharmacognosy identification are stable and reliable, which can be used as the basis for qualitative identification of gold buttons. Gold button medicine in processing, should pay attention to cleaning, so as not to bring too much sediment, and fully dry, to prevent moldy deterioration, in order to facilitate storage. High performance liquid chromatography (HPLC) was used to determine the content of Lv Yuan acid in gold button. The method is accurate, simple, rapid and reproducible. This study has established the experimental foundation for establishing the quality standard of the Zhuang medicine Jinjiu medicinal material and provided the scientific reference basis for its reasonable development and utilization in the future.
【学位授予单位】：广西中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R29

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