基于Oatp2a1表达探讨小鼠脾虚内环境与肝癌的关系

发布时间：2018-06-09 04:40

本文选题：有机阴离子转运肽a + 脾虚　；参考：《中华中医药杂志》2017年11期

【摘要】：目的:分析脾虚肝癌小鼠有机阴离子转运肽2a1(Oatp2a1)m RNA和蛋白的表达情况,探讨脾虚因素对肝癌的影响及其机制。方法:60只小鼠分成正常对照组、原位移植肝癌组和脾虚原位移植肝癌组,建立脾虚原位移植肝癌小鼠模型,测量各组肝癌肿瘤的体积,采用RT-PCR和Western Blot的方法检测小鼠各组织中Oatp2a1的表达。结果:脾虚原位移植肝癌组小鼠肝癌肿瘤体积明显大于原位移植肝癌组(P0.01),肝癌造模后小鼠各组织Oatp2a1的表达发生了变化,癌组织中Oatp2a1表达均明显高于相应组肝组织(P0.01),与原位移植肝癌组小鼠比较,脾虚原位移植肝癌组小鼠胃、肝和脾组织Oatp2a1 m RNA表达明显降低(P0.01),癌组织表达则明显升高(P0.01)。结论:脾虚因素能促进小鼠肝癌的发生和发展,其机制可能是脾虚内环境下各组织Oatp2a1的异常表达,进一步提示脾虚湿浊转运障碍与肝癌密切相关。
[Abstract]:Objective: to investigate the effect of spleen deficiency on liver cancer and its mechanism by analyzing the expression of mRNA and protein of organic anion transporter peptide 2a1Oatp2a1in mice with liver cancer due to spleen deficiency. Methods 60 mice were divided into normal control group, orthotopic liver transplantation group and spleen deficiency orthotopic transplantation group. The expression of Oatp2a1 in mouse tissues was detected by RT-PCR and Western blot. Results: the tumor volume of liver cancer in spleen deficiency orthotopic liver transplantation group was significantly larger than that in orthotopic transplantation liver cancer group (P 0.01). The expression of Oatp2a1 in various tissues of mice was changed after liver cancer model was established. The expression of Oatp2a1 in liver tissue was significantly higher than that in liver tissue of corresponding group. Compared with orthotopic liver transplantation group, the expression of Oatp2a1 mRNA in stomach, liver and spleen tissue of liver cancer group with spleen deficiency was significantly lower than that in liver tissue of liver cancer group, while the expression of Oatp2a1 mRNA in cancer tissue was significantly higher than that in orthotopic liver transplantation group. Conclusion: spleen deficiency can promote the occurrence and development of liver cancer in mice, and its mechanism may be the abnormal expression of Oatp2a1 in various tissues under the internal environment of spleen deficiency, which further indicates that the obstacle of transferring dampness due to spleen deficiency is closely related to liver cancer.
【作者单位】：中山大学附属第三医院中医科国家中医肝病重点专科广东省肝脏疾病研究重点实验室;中山大学附属第一医院中医科;
【基金】：国家自然科学基金面上项目(No.81072806,No.81373500,No.81673903) 广东省自然科学基金项目(No.S2012010009277)~~
【分类号】：R273

【相似文献】