朝医麻黄定喘汤对哮喘小鼠NF-κB信号通路的影响
发布时间：2018-08-04 09:28
【摘要】：目的:探讨朝医麻黄定喘汤对支气管哮喘小鼠气道重塑及核因子-κB(NF-κB)信号通路的影响。方法:50只清洁级雌性BABL/c小鼠,随机分为5组,每组10只,分别为正常对照组,模型组,麻黄定喘汤低、高剂量组,地塞米松组。以卵清蛋白致敏、激发制备小鼠哮喘模型,在末次激发24h后处死。取小鼠肺泡灌洗液(BALF)及肺组织检测炎性细胞的数量变化,白介素-4(IL-4)、白介素-5(IL-5)、白介素-13(IL-13)的水平及NF-κBp65蛋白的表达。结果:与正常对照组比较,模型组BALF中炎性细胞计数、IL-4、IL-5、IL-13水平均增高;肺组织NF-κBp65蛋白表达水平均显著升高(P0.01)。与模型组比较,麻黄定喘汤组小鼠BALF中炎性细胞计数、IL-4、IL-5、IL-13水平及NF-κBp65蛋白表达水平均显著降低(P0.05,P0.01),不同剂量麻黄定喘汤组间上述各指标间差异无统计学意义。结论:麻黄定喘汤可抑制哮喘小鼠气道重塑的发生,其机制可能是通过抑制NF-κB信号通路而实现的。
[Abstract]:Aim: to investigate the effect of Chaoyi mahuang Dingchuan decoction on airway remodeling and nuclear factor-魏 B (NF- 魏 B) signaling pathway in asthmatic mice. Methods Fifty female BABL/c mice of clean grade were randomly divided into 5 groups: normal control group, model group, ephedrine Dingchuan decoction low dose group, high dose group and dexamethasone group. Mouse asthma model was induced by sensitizing ovalbumin and killed 24 hours after the last stimulation. The changes of inflammatory cells, the levels of interleukin-4 (IL-4), interleukin-5 (IL-5), interleukin-13 (IL-13) and the expression of NF- 魏 Bp65 were measured in the alveolar lavage fluid (BALF) and lung tissue of mice. Results: compared with the normal control group, the inflammatory cell count and the level of IL-5 and IL-13 in the BALF of the model group were all increased, while the expression of NF- 魏 Bp65 protein in the lung tissue was significantly increased (P0.01). Compared with the model group, the level of IL-4, IL-5, IL-13 and the expression of NF- 魏 Bp65 in the BALF of the mice in the mahuang Dingchuan decoction group were significantly decreased (P0.05, P0.01), but there was no significant difference among the above indexes in the different dosage groups. Conclusion: mahuang Dingchuan decoction can inhibit airway remodeling in asthmatic mice and its mechanism may be by inhibiting NF- 魏 B signaling pathway.
【作者单位】： 延边大学;江南大学;
【基金】：国家自然科学基金项目(No.81560810) 吉林省教育厅“十三五”科学技术研究项目(No.吉教科合字[2016]第278号) 延边大学医药健康产品研发项目(No.延大科合字[2016]6号) 吉林省科技发展计划项目(No.20140204046YY)~~
【分类号】：R29
本文编号：2163403
[Abstract]:Aim: to investigate the effect of Chaoyi mahuang Dingchuan decoction on airway remodeling and nuclear factor-魏 B (NF- 魏 B) signaling pathway in asthmatic mice. Methods Fifty female BABL/c mice of clean grade were randomly divided into 5 groups: normal control group, model group, ephedrine Dingchuan decoction low dose group, high dose group and dexamethasone group. Mouse asthma model was induced by sensitizing ovalbumin and killed 24 hours after the last stimulation. The changes of inflammatory cells, the levels of interleukin-4 (IL-4), interleukin-5 (IL-5), interleukin-13 (IL-13) and the expression of NF- 魏 Bp65 were measured in the alveolar lavage fluid (BALF) and lung tissue of mice. Results: compared with the normal control group, the inflammatory cell count and the level of IL-5 and IL-13 in the BALF of the model group were all increased, while the expression of NF- 魏 Bp65 protein in the lung tissue was significantly increased (P0.01). Compared with the model group, the level of IL-4, IL-5, IL-13 and the expression of NF- 魏 Bp65 in the BALF of the mice in the mahuang Dingchuan decoction group were significantly decreased (P0.05, P0.01), but there was no significant difference among the above indexes in the different dosage groups. Conclusion: mahuang Dingchuan decoction can inhibit airway remodeling in asthmatic mice and its mechanism may be by inhibiting NF- 魏 B signaling pathway.
【作者单位】： 延边大学;江南大学;
【基金】：国家自然科学基金项目(No.81560810) 吉林省教育厅“十三五”科学技术研究项目(No.吉教科合字[2016]第278号) 延边大学医药健康产品研发项目(No.延大科合字[2016]6号) 吉林省科技发展计划项目(No.20140204046YY)~~
【分类号】：R29
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