拨法对CCI大鼠脊髓中IL-6及SOCS3的影响

发布时间：2018-10-30 14:04

【摘要】：目的从炎症反应的角度探索拨法对神经病理性疼痛大鼠的影响。方法采用推拿手法模拟仪定性、定量模拟拨法,对CCI大鼠进行干预,通过光热耐痛阈观察大鼠行为学的改善情况,通过免疫组化观察大鼠脊髓中IL-6及SOCS3表达的变化。结果造模7d后,模型组大鼠光热耐痛阈结果与正常组相比有显著差异(P0.01),模型组大鼠脊髓中IL-6及SOCS3的表达较正常组显著升高(P0.01);拨法组用拨法治疗20次后,大鼠光热耐痛阈结果与同期模型组相比有显著差异(P0.05),且逐渐趋向于正常水平,大鼠脊髓中IL-6的表达较同期模型组有显著降低(P0.01),脊髓中SOCS3的表达较同期模型组有显著性升高(P0.01)。结论拨法可一定程度上改善神经病理性疼痛大鼠的痛觉和温度觉,同时可抑制大鼠体内促炎症因子的表达。
[Abstract]:Objective to explore the effect of dipping on neuropathic pain rats from the angle of inflammatory reaction. Methods qualitative and quantitative simulation of massage manipulation were used to interfere with CCI rats. The improvement of behavior was observed by photothermal pain tolerance threshold and the changes of IL-6 and SOCS3 expression in spinal cord were observed by immunohistochemistry. Results after 7 days, the results of photothermal pain tolerance in the model group were significantly different from those in the normal group (P0.01). The expression of IL-6 and SOCS3 in the spinal cord of the model group was significantly higher than that of the normal group (P0.01). After 20 times of treatment, the results of photothermal pain tolerance threshold of rats were significantly different from those of the model group (P0.05), and gradually tended to normal level. The expression of IL-6 in the spinal cord of rats was significantly lower than that in the model group (P0.01), and the expression of SOCS3 in the spinal cord was significantly higher than that in the model group (P0.01). Conclusion Diaphora can improve the pain and temperature sensation of rats with neuropathic pain to some extent, and inhibit the expression of pro-inflammatory factors in rats.
【作者单位】：北京中医药大学针灸推拿学院;
【基金】：国家自然科学基金青年基金(81403497);国家自然科学基金(81373759)
【分类号】：R244.1

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