肋柱花不同溶剂提取物抗急性肝损伤药理作用研究

发布时间：2018-11-04 11:56

【摘要】：目的:通过蒙药肋柱花不同溶剂提取物及活性部位配伍药理作用研究,筛选肋柱花抗炎、镇痛、抗急性肝损伤有效活性部位。方法:将小鼠按体重分成若干组,通过二甲苯耳肿胀抗炎实验,计算肿胀度和肿胀抑制率(%);通过醋酸扭体实验,记录小鼠开始扭体时间和内扭体次数;通过抗D-氨基半乳糖胺急性肝损伤药理实验,检测小鼠血清ALT、AST,观察肝组织病理变化。结果:1.肋柱花石油醚、乙酸乙酯、乙醇提取物具有一定的降低小鼠耳肿胀度,p0.05;肋柱花石油醚、二氯甲烷、乙酸乙酯、乙醇提取部位具有一定的抑制小鼠耳肿胀作用。2.肋柱花石油醚、乙醇提取物具有一定的延长小鼠开始扭体时间和减少扭体次数,p0.05。3.肋柱花石油醚、乙酸乙酯、乙醇提取物具有一定的降低血清AST作用,p0.05;肋柱花乙酸乙酯、乙醇提取物具有一定的降低血清ALT作用,p0.05。4.肋柱花石油醚:乙醇提取物配伍组(1:9);二氯甲烷:乙酸乙酯:乙醇提取物配伍组(1:2:9)具有一定的降低小鼠血清AST、ALT作用,p0.05或p0.01。5.肋柱花石油醚:乙醇(1:9)配伍组大、中、小剂量组具有一定的降低小鼠耳肿胀度和抑制小鼠耳肿胀作用;肋柱花石油醚:乙醇(1:9)配伍组大、中剂量组具有一定的延长小鼠开始扭体时间和减少扭体次数,p0.05;肋柱花石油醚:乙醇(1:9)配伍组大、中剂量组具有一定的降低血清AST、ALT作用,p0.05。结论:1.肋柱花石油醚、乙酸乙酯、乙醇提取物具有一定的抗炎活性。2.肋柱花石油醚、乙醇提取物具有一定的镇痛作用。3.肋柱花石油醚、乙酸乙酯、乙醇提取物具有一定的保肝活性。4.肋柱花石油醚:乙醇提取物配伍组(1:9);二氯甲烷:乙酸乙酯:乙醇提取物配伍组(1:2:9)具有显著的保肝活性。5.肋柱花石油醚:乙醇(1:9)配伍组大、中、小剂量组具有一定的抗炎、镇痛、保肝活性。6.通过以上实验筛选出蒙药肋柱花抗炎、镇痛、保肝有效活性部位,为进一步提取肋柱花保肝活性成分提供科学依据。
[Abstract]:Objective: to study the pharmacological effects of different solvent extracts and active fractions of Lumbellae mongolicum on anti-inflammatory, analgesic and anti-acute liver injury. Methods: mice were divided into several groups according to their body weight. The swelling degree and swelling inhibition rate (%) were calculated by xylene ear swelling and anti-inflammatory test, and the time of writhing and the times of internal writhing were recorded by acetic acid writhing test. The pathological changes of liver tissue were detected by ALT,AST, in serum of mice by pharmacological experiment of anti-D-galactosamine acute liver injury. The result is 1: 1. The extract of petroleum ether, ethyl acetate and ethanol could reduce the ear swelling in mice (p0.05), and the extracts of petroleum ether, dichloromethane, ethyl acetate and ethanol could inhibit the ear swelling in mice. 2. The petroleum ether and ethanol extract of Flos costolata could prolong the time of writhing and reduce the times of writhing in mice (p0.05.3). The extracts of petroleum ether, ethyl acetate and ethanol had a certain effect on reducing serum AST (p0.05), while ethyl acetate and ethanol extracts from costal flower had a certain effect on reducing serum ALT (p0.05.4). The mixture group of petroleum ether and ethanol extract (1:9) and dichloromethane: ethyl acetate: ethanol extract (1:2:9) had the effect of decreasing serum AST,ALT in mice (p0.05 or p0.01.5). The large, medium and low dose groups of petroleum ether: ethanol (1:9) could reduce the degree of ear swelling in mice and inhibit the swelling of ears in mice to a certain extent. The combination of ethanol (1:9) and petroleum ether (1:9) was large, and the middle dose group could prolong the time of writhing and reduce the times of writhing in mice (p0.05). The effect of petroleum ether: ethanol (1:9) on serum AST,ALT was higher in the middle dose group than that in the middle dose group (p0.05). Conclusion: 1. The extracts of petroleum ether, ethyl acetate and ethanol have certain anti-inflammatory activity. 2. The extract of petroleum ether and ethanol has a certain analgesic effect. 3. The extracts of petroleum ether, ethyl acetate and ethanol have certain hepatoprotective activity. 4. The compatibility group of petroleum ether and ethanol extract (1:9) and dichloromethane: ethyl acetate: ethanol extract (1:2:9) had remarkable hepatoprotective activity. The large, medium and low dose groups of petroleum ether: ethanol (1:9) had some anti-inflammatory, analgesic and hepatoprotective activities. Through the above experiments, the effective active parts of the Mongolian medicine costal flower resistance, analgesia and liver preservation were selected, which provided scientific basis for the further extraction of liver-preserving active components from the costal style.
【学位授予单位】：内蒙古民族大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R29

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