艾灸神阙穴对高脂血症小鼠调脂控炎的效应研究

发布时间：2018-12-11 14:23

【摘要】：目的：观察艾灸神阙穴对高脂血症小鼠血脂的调节和对炎症的控制效应,通过检测血脂指标和血清炎症因子的水平,进一步探讨血脂代谢异常和炎症反应的关联性。以期从调脂控炎的角度探讨防治动脉粥样硬化等慢性炎症性疾病。方法：40只2月龄雄性C57BL/6J小鼠被随机分为4组,每10只一组,分别为正常组、模型组、艾灸组、药物组。正常组给予普通饮食,其余各组均采用自由饮食高脂饲料6周造模成高脂血症小鼠模型。艾灸组每天予以艾条灸神阙穴10 min,药物组每天予以洛伐他汀(10mg/kg)混悬液灌胃,两组均治疗两周,正常组、模型组未予以其他特殊治疗。治疗结束后取各组小鼠血清进行指标检测,用酶标仪比色法检测血清胆固醇(TC)、甘油三酯(TG)含量,用双抗体夹心法检测血清白细胞介素-6(IL-6)、白细胞介素-10(IL-10)、TOLL样受体2(TLR2)、肿瘤坏死因子-α(TNF-α)和血清内皮素1(ET-1)的水平。结果：①血脂指标：与正常组比较,模型组小鼠血清TC、TG水平明显升高,且差异具有显著性(P0.01),这说明造模成功。与模型组比较,艾灸组和药物组小鼠血清TC、TG水平均明显降低,且差异具有显著性(P0.01)。艾灸组和药物组比较TC、TG水平变化无统计学差异(P值分别为P=0.332、P=0.849)。②血清炎性指标：与模型组比较,艾灸组和药物组两组血清IL-6、TNF-α、TLR2、ET-1水平均显著降低(P0.05),血清IL-10水平明显升高(P0.05),差异均具有统计学意义。在降低血清IL-6水平和升高IL-10水平方面,艾灸组稍优于药物组,但此差异并无统计学意义(P值分别为P=0.91、P=1.000)。在降低血清TNF-α、TLR2水平方而,药物组略优于艾灸组,但此差异并无统计学意义(P值分别P=0.749、P=0.217)。在降低血清ET-1水平方而,药物组与艾灸组无统计学差异(P=0.993)。结论：艾灸神阙穴对高脂血症有良好的调脂控炎的效应,能够降低血脂水平,而且能降低血清TNF-α、IL-6、TLR2、ET-1炎症因子水平,升高抑炎因子IL-10水平。艾灸可通过其温通效应调脂、控炎,对动脉粥样硬化等慢性炎症性疾病具有良好的防治作用。
[Abstract]:Objective: to observe the effect of moxibustion at Shenque point on blood lipids and inflammation in hyperlipidemic mice, and to explore the relationship between abnormal metabolism of blood lipids and inflammatory reaction by detecting the indexes of blood lipids and the level of serum inflammatory factors. The aim of this study is to discuss the prevention and treatment of chronic inflammatory diseases such as atherosclerosis from the point of regulating lipid and controlling inflammation. Methods: forty 2 month old male C57BL/6J mice were randomly divided into 4 groups: normal group, model group, moxibustion group and drug group. The normal diet was given to the normal group and the other groups were made into hyperlipidemia mice model with free diet and high fat diet for 6 weeks. The moxibustion group was given daily moxibustion at Shenque point for 10 min,. The moxibustion group was given lovastatin (10mg/kg) suspension daily. The two groups were treated for two weeks. The normal group and the model group were not given other special treatment. After treatment, serum levels of cholesterol (TC), triglyceride (TC),) and serum interleukin-6 (IL-6) were detected by enzyme colorimetry and double antibody sandwich method, respectively. Levels of interleukin-10 (TLR2), tumor necrosis factor- 伪 (TNF- 伪) and serum endothelin 1 (ET-1). Results: 1 Blood lipid index: compared with the normal group, the serum TC,TG level in the model group was significantly higher than that in the normal group (P0.01), which indicated that the model was successful. Compared with the model group, the serum TC,TG level in moxibustion group and drug group was significantly decreased (P0.01). There was no significant difference in TC,TG level between moxibustion group and drug group (P = 0.332P0. 849). 2 Serum inflammatory index: compared with model group, serum IL-6,TNF- 伪 and TLR2, in moxibustion group and drug group were higher than those in moxibustion group and drug group respectively. ET-1 levels were significantly decreased (P0.05), serum IL-10 levels were significantly increased (P0.05), the differences were statistically significant. In decreasing serum IL-6 level and increasing IL-10 level, the moxibustion group was slightly better than the drug group, but the difference was not statistically significant (P = 0.91%, P = 1.000). In decreasing serum TNF- 伪 and TLR2 levels, the drug group was slightly better than the moxibustion group, but the difference was not statistically significant (P = 0.749, P = 0.217, respectively). There was no significant difference between the drug group and moxibustion group in reducing serum ET-1 level (P0. 993). Conclusion: moxibustion at Shenque point has a good effect on hyperlipidemia, which can decrease the level of blood lipid, reduce the levels of serum TNF- 伪 and IL-6,TLR2,ET-1 inflammatory factors, and increase the level of IL-10. Moxibustion can regulate lipid and inflammation through its warming effect and has good preventive and therapeutic effect on chronic inflammatory diseases such as atherosclerosis.
【学位授予单位】：南京中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R245

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