针刺对慢性应激焦虑模型大鼠催产素及其受体的作用研究

发布时间：2019-03-24 14:31

【摘要】：目的：本课题在前期研究针刺抗焦虑效应并对ANP-HPA轴有调控作用的基础上,进一步拓展研究针刺对慢性应激焦虑大鼠下丘脑催产素(Oxytocin, OT)及其受体(Oxytocin Rceptor, OXTR)的作用以及下丘脑-垂体-肾上腺轴(hypothalamo-p ituitary-adrenal axis, HPA轴)末端皮质酮(corticosterone, CORT)水平的影响,探讨针剌治疗焦虑障碍的部分神经内分泌作用机理,拟为针刺抗焦虑的机理研究拓展新方向。方法：选用健康、清洁级、雄性SD大鼠30只,随机分为空白组、模型组、针刺组,每组10只。采用不可预知的慢性应激(chronic unpredictable stress, CUS)法建立焦虑动物模型。针刺组选取“内关”、“神门”穴进行手针治疗,5分钟捻转一次,捻转持续30s,隔日一次,左右交替进行,每次治疗持续30min,持续15天,针刺于造模当天开始。采用高架十字迷宫测试大鼠焦虑样行为,运用酶联免疫法测定血浆中的OT和CORT的含量,采用免疫组化测定下丘脑OT和OXTR的表达。结果：1.行为学：与空白组比较,模型组高架十字迷宫0T%值明显降低(P0.05)；与模型组比较,针刺组0T%值明显升高(P0.05)。模型组0E%较空白组降低(P0.05),针刺组0E%也较模型组升高(P0.05),但组间比较无统计学差异。表明实验模型动物表现为焦虑状态,造模成功,而针刺可以改善动物的焦虑情绪。2.下丘脑OT、OXTR:与空白组比较,模型组下丘脑OT的浓度较空白组显著降低(P0.01)；针刺组较模型组下丘脑OT浓度显著升高(P0.01)),组间差异均有统计学意义。模型组OXTR浓度相对于空白组有所降低(P0.05),针刺组OXTR浓度较模型组也有降低,但组间比较无统计学差异(P0.05)。表明针刺可以上调慢性应激焦虑模型大鼠的OT浓度。3.血浆OT：与空白组比较,模型组血浆OT浓度降低；与模型组比较,针刺组血浆OT浓度升高,但组间比较未显统计学意义(P0.05)。4.血浆CORT:模型组相比空白组,血浆CORT浓度显著升高(P0.01)；针刺组相比模型组,血浆CORT浓度明显降低(P0.05)。说明慢性应激导致大鼠CORT升高,针刺可以下调应激导致的过高的CORT。结论：针刺可以改善慢性应激大鼠的焦虑情绪,这可能与针刺上调下丘脑OT表达,降低血浆CORT水平、调节HPA轴功能有关。
[Abstract]:Objective: to explore the effect of acupuncture on hypothalamic oxytocin (Oxytocin, OT) and its receptor (Oxytocin Rceptor, in rats with chronic stress anxiety on the basis of the preliminary study on the anti-anxiety effect of acupuncture and the regulating effect of acupuncture on ANP-HPA axis in rats with chronic stress anxiety. The effects of OXTR and the (corticosterone, CORT) level of corticosterone at the end of the hypothalamic-pituitary-adrenal axis (hypothalamo-p ituitary-adrenal axis, HPA axis) were studied to explore the mechanism of some neuroendocrine effects of acupuncture in the treatment of anxiety disorder. It is intended to open up a new direction for the study of the mechanism of acupuncture anti-anxiety. Methods: 30 healthy, clean and male SD rats were randomly divided into blank group, model group and acupuncture group with 10 rats in each group. The anxiety animal model was established by unpredictable chronic stress (chronic unpredictable stress, CUS) method. Acupuncture group selected "Neiguan", "Shenmen" point for hand-needle treatment, twisting once in 5 minutes, twisting lasted 30 s, alternate day, alternately, each treatment lasted 30 minutes, lasted 15 days, acupuncture began on the same day. The anxiety-like behavior of rats was measured by elevated cruciform maze, the contents of OT and CORT in plasma were measured by enzyme linked immunosorbent assay (Elisa), and the expression of OT and OXTR in hypothalamus was measured by immunohistochemistry. Results: 1. Behavior: compared with the blank group, the 0T% value of the model group was significantly lower than that of the control group (P0.05), and the 0T% value of the acupuncture group was significantly higher than that of the model group (P0.05). The 0E% of the model group was lower than that of the blank group (P0.05), and the 0E% of the acupuncture group was also higher than that of the model group (P0.05), but there was no statistical difference between the two groups. The results show that the experimental model animal is in a state of anxiety, and the model is successful, and acupuncture can improve the anxiety of the animal. 2. Compared with the blank group, the hypothalamus OT,OXTR: concentration in the model group was significantly lower than that in the blank group (P0.01), and the OT concentration in the hypothalamus in the acupuncture group was significantly higher than that in the model group (P0.01), group was significantly higher than that in the model group). The concentration of OXTR in the model group was lower than that in the blank group (P0.05), and the concentration of OXTR in the acupuncture group was also lower than that in the model group, but there was no statistical difference between the two groups (P0.05). It is suggested that acupuncture can up-regulate the concentration of OT in rats with chronic stress anxiety. 3. Compared with the blank group, the plasma OT: concentration in the model group was lower than that in the model group, and the plasma OT concentration in the acupuncture group was higher than that in the model group, but there was no significant difference between the two groups (P0.05). The plasma CORT: concentration in the model group was significantly higher than that in the blank group (P0.01), and the plasma CORT concentration in the acupuncture group was significantly lower than that in the model group (P0.05). It is suggested that chronic stress can lead to the increase of CORT in rats, and acupuncture can down-regulate the excessive CORT. induced by stress. Conclusion: acupuncture can improve the anxiety of chronic stress rats, which may be related to the up-regulation of OT expression in hypothalamus, the decrease of plasma CORT level and the regulation of HPA axis function.
【学位授予单位】：成都中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R245;R-332

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