HPLC法同时测定蒙药复方述达格-4中四个黄酮类成分的含量
发布时间：2019-04-27 13:18
【摘要】：建立同时测定蒙药复方述达格-4中山奈酚、高良姜素、山奈素、高良姜素-3-甲醚含量的方法.采用HPLC法,色谱柱为AgilentC18色谱柱(4.6mm×250mm,5μm),流动相乙腈-0.2%乙酸水溶液(梯度洗脱),流速0.6mL·min-1,检测波长254nm,柱温30℃,进样量为10μL.得出山奈酚、高良姜素、山奈素和高良姜素-3-甲醚线性范围分别为0.00067-0.00670μg(r=0.9999)、0.5240-5.2400μg(r=0.9999)、0.1484-1.4840μg(r=0.9999)、0.0996-0.9960μg(r=0.9999);精密度、稳定性、重复性试验的RSD2%;平均加样回收率分别为96.79%(RSD=1.70%,n=6)、97.38%(RSD=1.59%,n=6)、100.96%(RSD=1.49%,n=6)、97.01%(RSD=1.50%,n=6).结果表明该方法操作简便、稳定、重复性好,可用于蒙药复方述达格-4中4个黄酮成分含量的测定.本研究可为蒙药复方述达格-4资源开发利用提供理论依据.
[Abstract]:A method was developed for the simultaneous determination of Zhongshan phenol, kaempferol, kaempferol and galangin-3-methyl ether in Mongolian medicine compound Zudag-4. A HPLC method was used with a AgilentC18 column (4.6mm 脳 250 mm, 5 渭 m), a mobile phase acetonitrile-0.2% acetic acid solution (gradient elution), a flow rate of 0.6mL 路min-1, a detection wavelength of 254 nm, a column temperature of 30 鈩，
本文编号：2467017
[Abstract]:A method was developed for the simultaneous determination of Zhongshan phenol, kaempferol, kaempferol and galangin-3-methyl ether in Mongolian medicine compound Zudag-4. A HPLC method was used with a AgilentC18 column (4.6mm 脳 250 mm, 5 渭 m), a mobile phase acetonitrile-0.2% acetic acid solution (gradient elution), a flow rate of 0.6mL 路min-1, a detection wavelength of 254 nm, a column temperature of 30 鈩，
本文编号：2467017
本文链接：https://www.wllwen.com/zhongyixuelunwen/2467017.html
