北京地区7种常见嗜尸性蝇类的COI基因序列分析及DNA条形码的建立

发布时间：2019-05-11 05:42

【摘要】：嗜尸性蝇类在命案死亡时间和现场推断方面有着十分重要的应用,而DNA条形编码技术能摆脱对虫卵和幼虫的饲养以及后续物种鉴定方面专业知识的依赖,有助于实现现场采集蝇类样本的快速鉴定。本研究采集了北京地区7个嗜尸性蝇类优势种共77个个体的样本,测定了所有个体线粒体DNA上细胞色素C氧化酶亚基I(COI)基因1 120bp的序列。基于序列的系统发生分析显示,同一物种不同个体的序列均以高达99%的支持值聚集在一起。序列间的分歧统计表明这些蝇类在物种内的个体分歧不超过1%,而不同物种间的净分歧均超过7.74%,最高可达14.85%。滑动窗口分析表明,在整个序列区段种间差异位点存在较平均的分布。通过测定COI基因的序列,建立了北京地区7个嗜尸性蝇类优势种的DNA条形码,据此实现了对这些物种准确、快速、简单的区分和鉴定,同时也为后续应用于物种鉴定的种属特异性位点之筛选提供了基础数据。
[Abstract]:Cadaveric flies play a very important role in the time of death and field inference, and DNA bar coding technology can get rid of the dependence on the professional knowledge of egg and larva breeding and subsequent species identification. It is helpful to realize the rapid identification of fly samples collected in the field. In this study, 77 individuals from 7 dominant species of cadaveric flies in Beijing were collected, and the sequence of I (COI) gene 1 120bp of cytochrome C oxidase subunit in all individuals was determined. Sequence-based phylogenetic analysis showed that the sequences of different individuals of the same species gathered together with support values of up to 99%. The statistics of sequence divergence showed that the individual differences of these flies in species were less than 1%, while the net differences among different species were more than 7.74%, and the highest was 14.85%. Sliding window analysis showed that there was an average distribution of interspecific difference sites in the whole sequence. By sequencing the COI gene, the DNA bar codes of seven dominant species of cadaveric flies in Beijing were established, and the accurate, rapid and simple identification of these species was realized. At the same time, it also provides the basic data for the screening of species specific loci used in species identification.
【作者单位】：中国科学院研究生院;北京市公安局刑侦总队法医检验鉴定中心;中国科学院北京基因组研究所;中国科学院动物研究所;
【基金】：公安部科技创新项目(20061B0400100113)
【分类号】：D919

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