基于荧光标记SSR的CID法快速鉴定福建茶树品种

发布时间：2018-06-16 01:41

本文选题：茶树 + SSR　；参考：《茶叶科学》2016年02期

【摘要】：茶树品种真实性鉴定是茶树种质资源研究的重要组成部分,是茶树品种知识产权保护的前提。在茶树基因组上按照均匀分布的原则选择45个SSR标记合成荧光标记引物,利用这些标记引物对54个福建无性系茶树品种进行PCR扩增,标记基因型分型后进一步筛选出9个SSR标记核心引物。这批核心引物的多态性信息含量(Polymorphism information content,PIC)均大于0.5,等位位点数在3~5之间,基因型数在5~10之间,属于高度多态性引物且方便进行基因型数据统计,适宜用来进行无性系茶树品种的真实性鉴定。利用其中的6个标记核心引物建立了54个福建无性系茶树品种的品种鉴别图(Cultivar identification diagram,CID),该CID能直观地提供对其中任意品种进行鉴定所需要的标记引物以及相应的标记基因型,且能够方便地进行扩容以用来对更多的无性系茶树品种进行真实性鉴定。利用该方法对无性系茶树品种进行真实性鉴定,操作方便、快速准确、稳定性高、实用性强。本实验所获得的茶树品种鉴定图(CID)对茶树品种知识产权保护,以及促进茶树品种选育工作健康可持续发展具有重要的意义。
[Abstract]:The authenticity identification of tea varieties is an important part of tea germplasm resources research and the premise of intellectual property protection of tea varieties. In accordance with the principle of uniform distribution, 45 SSR markers were selected to synthesize fluorescent marker primers on tea genome, and 54 Fujian clones were amplified by PCR using these markers. After genotyping, 9 core primers of SSR markers were screened out. The polymorphic information polymorphism information content of these core primers were all greater than 0.5, the allele number was between 3 and 5, and the number of genotypes was between 5 and 10, which belonged to highly polymorphic primers and was convenient for genotypic data statistics. It is suitable to be used to identify the truthfulness of clonal tea varieties. Using the 6 marker core primers, the cultivar identification map of 54 Fujian clone tea varieties, Cultivar identification diagramramma, was established. The CID could provide the marker primers and the corresponding marker genotypes needed for the identification of any of them. And it can be easily expanded to identify the authenticity of more clones tea varieties. The method was used to identify the truthfulness of clonal tea varieties, which was easy to operate, fast and accurate, high stability and strong practicability. The identification map of tea varieties obtained in this experiment has important significance for the protection of intellectual property rights of tea varieties and for promoting the healthy and sustainable development of tea variety breeding.
【作者单位】：福建省农业科学院茶叶研究所/国家茶树改良中心福建分中心;
【基金】：福建省自然科学基金(2015J01098) 福建省省属公益类科研院所基本科研专项(2014R1012-6) 福建省农业科学院茶叶科技创新团队项目(CXTD-1-1302) 安徽农业大学茶树生物学与资源利用国家重点实验室开放基金(LTBB20140102)
【分类号】：S571.1

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