基于SSR的刺槐无性系遗传多样性分析和指纹图谱构建
发布时间：2019-06-02 18:03
【摘要】：【目的】对山东省刺槐无性系进行遗传多样性分析和指纹图谱构建,为刺槐新品种选育、遗传改良和品种鉴定提供可靠的依据,为中国其他省市刺槐遗传资源保存、评价与利用提供参考。【方法】采用荧光SSR引物进行PCR扩增,产物经毛细管电泳仪进行检测;利用所得结果分析49个无性系遗传多样性并构建其指纹图谱;采用非加权组平均法(UPGMA)进行基于亲缘关系的无性系聚类分析。【结果】8对SSR引物共检测到51个等位基因,每个位点的等位基因为2~15个,平均每对引物扩增出6.375个多态性等位基因。不同位点的PIC值变化范围很大,在0.092~0.879之间,平均PIC值为0.509 8。使用组间平均数联结法进行UPGMA聚类分析,结果表明49份无性系没有严格按照不同区域聚类到一起,无性系分组与现有栽培区没有明显的规律。来自临沂的‘鲁刺8’和‘鲁刺13’、青岛的‘鲁刺40’和‘鲁刺42’以及日照的‘鲁刺10’和‘鲁刺86’亲缘关系最近。本研究所使用的9对引物中,其中2对Rply109和rops16可以区分开45份无性系,鉴定率达到91.84%。检测到22个刺槐无性系在1个或者2个位点得到3个等位基因,表明这些无性系可能是发生自然变异的多倍体。【结论】山东省刺槐具有较高的遗传多样性。无性系分组与现有栽培区没有明显的规律。引物Rply109和rops16对49份无性系的分子鉴定率为91.84%,可作为刺槐指纹图谱构建和分子鉴定的高效分子标记。利用SSR标记构建的指纹图谱可为刺槐遗传资源管理、品种鉴定和知识产权保护奠定坚实的基础,同时为刺槐的引种和遗传育种亲本选择提供科学的理论依据。
[Abstract]:[objective] to analyze the genetic diversity and construct fingerprint of Robinia pseudoacacia clones in Shandong Province, so as to provide reliable basis for breeding, genetic improvement and variety identification of new Robinia pseudoacacia varieties, and to preserve the genetic resources of Robinia pseudoacacia in other provinces and cities of China. The evaluation and utilization provided reference. [methods] fluorescent SSR primers were used for PCR amplification, and the products were detected by capillary electrophoresis. The genetic diversity of 49 clones was analyzed and their fingerprints were constructed. Unweighted group average method (UPGMA) was used to cluster analysis of clones based on genetic relationship. [results] A total of 51 alleles were detected by 8 pairs of SSR primers, and the number of alleles per locus was 2 鈮，
本文编号：2491316
[Abstract]:[objective] to analyze the genetic diversity and construct fingerprint of Robinia pseudoacacia clones in Shandong Province, so as to provide reliable basis for breeding, genetic improvement and variety identification of new Robinia pseudoacacia varieties, and to preserve the genetic resources of Robinia pseudoacacia in other provinces and cities of China. The evaluation and utilization provided reference. [methods] fluorescent SSR primers were used for PCR amplification, and the products were detected by capillary electrophoresis. The genetic diversity of 49 clones was analyzed and their fingerprints were constructed. Unweighted group average method (UPGMA) was used to cluster analysis of clones based on genetic relationship. [results] A total of 51 alleles were detected by 8 pairs of SSR primers, and the number of alleles per locus was 2 鈮，
本文编号：2491316
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